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[1]刘 冉,尹立红,浦跃朴,等.食管组织中代谢酶相关基因的异常表达与 食管癌的发病关系研究[J].医学研究与战创伤救治(原医学研究生学报),2008,10(06):401.
　LIU Ran,YIN Li-hong,PU Yue-pu,et al.Association between metabolism gene expression in esophageal tissues and the risk for esophageal carcinomas[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(06):401.
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食管组织中代谢酶相关基因的异常表达与 食管癌的发病关系研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年06期
页码:
401
栏目:
出版日期:
2008-11-20

文章信息/Info

Title:
Association between metabolism gene expression in esophageal tissues and the risk for esophageal carcinomas
文章编号:
1672-271X(2008)06-0401-05
作者:
刘 冉1; 尹立红1; 浦跃朴1; 王 仪2; 潘恩春2
1.东南大学公共卫生学院,江苏南京 210009; 2.淮安市疾病预防控制中心, 江苏淮安 223001
Author(s):
LIU Ran1;  YIN Li-hong1;  PU Yue-pu1;  Wang Yi2;  Pan En-chun2
1.School of Public Health, Southeast University, Nanjing 210009,Jiangsu,China; 2. Huaian Center for Disease Control and Prevention, Huai’an 223001,Jiangsu,China
关键词:
食管癌; 代谢酶; 基因表达; 实时荧光定量PCR
Keywords:
Esophageal carcinomas;  Metabolism gene; Gene expression;  Quantitative real time RT-PCR
分类号:
R735.1
DOI:
-
文献标志码:
A
摘要:
目的 研究与环境致癌物活化、解毒有关的Ⅰ相和Ⅱ相代谢酶的mRNA水平与食管癌发病的关系。方法 收集93例淮安地区食管癌新发病例的食管癌组织及远端癌旁组织，应用SYBR Green Ⅰ实时荧光定量逆转录-聚合酶链反应(RT-PCR) 检测CYP2E1、GSTP1、MTHFR和NQO1等基因的mRNA水平；应用t检验和logistic回归分析评价基因的表达水平与食管癌的关系。结果 CYP2E1和MTHFR的mRNA水平在癌组织和配对癌旁组织之间有显著差异；条件logistic逐步回归分析结果显示CYP2E1、MTHFR表达水平异常与食管癌发病风险升高有关联[OR值(95%CI)分别为1.890 (1.439~2.481)和1.362 (1.107~1.677)]。结论 CYP2E1和MTHFR的表达水平改变与食管癌发病风险密切相关，在食管癌的高危人群筛查和早期诊断中具有重要意义。
Abstract:
Objective To investigate the relation between the mRNA expression levels of genes that involved in Ⅰ and Ⅱ phase of metabolism and activation of environmental carcinogens and the risk for ESCC. Methods Tumor and no-cancer tissues from 93 patients of newly diagnosed, untreated ESCC were collected in Huai'an County. The relative expression levels of four metabolism genes (CYP2E1, GSTP1, MTHFR and NQO1) were determined in cancer and no-cancer tissues by quantitative real-time RT-PCR using SYBR Green I. The expression of β-actin was used as an internal control. T-test and logistical regression analysis were used to test the differences of gene expression between cancer tissues and tissues adjacent to the tumors at a significance level of 5%.Results Compared with tissues adjacent to the tumors, the relative expression levels of CYP2E1 and MTHFR were significantly altered in esophageal cancer tissues. The conditional logistic regression analysis exhibited that the increased risk for esophageal cancer were significantly associated with aberrant expression of CYP2E1 and MTHFR [OR (95%CI)= 1.890 (1.439~2.481), OR (95%CI)=1.362 (1.107~1.677), respectively].Conclusion The increased expression of CYP2E1 and the reduced expression of MTHFR in esophagus were significantly associated with the risk for ESCC. Our data suggest an important etiology clue to CYP2E1 and MTHFR expression for the early progression of ESCC.

参考文献/References:

[1]陈建国, 陆建华. 国内外癌症防制现状[J]. 肿瘤, 2007, 27(9): 755-759.
[2]林东昕, 谭 文, 陆士新, 等. 中国食管癌分子流行病学研究[J]. 中华流行病学杂志,2003,24(10): 939-943.
[3]Vallbohmer D, Lenz HJ. Predictive and prognostic molecular markers in outcome of esophageal cancer[J]. Dis Esophagus, 2006, 19(6):425-432.
[4]Spivack SD, Hurteau GJ,Fasco MJ,et al.Phase I and II carc- inogen metabolism gene expression in human lung tissue and tumors[J]. Clin Cancer Res， 2003, 9(16 Pt 1):6002-6011.
[5]Horiike N, Abe M, Kumagi T, et al. The quantification of cytochrome P-450 (CYP 3A4) mRNA in the blood of patients with viral liver diseases[J]. Clin Biochem， 2005,38(6):531-534.
[6]Strassburg A, Strassburg CP, Manns MP, et al. Differential gene expression of NAD(P)H:quinone oxidoreductase and NRH:quinone oxidoreductase in human hepatocellular and biliary tissue[J]. Mol Pharmacol， 2002, 61(2):320-325.
[7]Livak KJ, Schmittgen TD. Analysis of relative gene expres-sion data using real-Time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001,25(4):402-408.
[8]Liu R,Yin LH,Pu YP. Association of combined CYP2E1 gene polymorphism with the risk for esophageal squamous cell carcinoma in Huai'an population, China[J]. Chin Med J (Engl), 2007, 120(20):1797-1802.
[9]Godoy W, Albano RM, Moraes EG, et al. CYP2A6/2A7 and CYP2E1 expression in human oesophageal mucosa: regional and inter-individual variation in expression and relevance to nitrosamine metabolism[J]. Carcinogenesis， 2002, 23(4):611-616.
[10]王 江, 张三申,文小岗,等. 食管癌发生发展过程中GST-π基因表达的研究[J]. 中华肿瘤杂志, 1999, 21(1):29-31.
[11]Brabender J, Lord RV, Wickramasinghe K, et al. Glutathi- one S-Transferase-Pi expression is downregulated in patients with Barrett's esophagus and esophageal adenocarcinoma[J]. J Gastrointest Surg, 2002,6(3):359-367.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(30571540，30671732)；江苏省预防医学基金项目(Y2006007)
更新日期/Last Update: 2013-11-22