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[1]张胜行,兰小鹏,易浔飞.靶向DcR3的siRNA抑制人结肠癌 裸鼠皮下移植瘤的实验研究[J].医学研究与战创伤救治(原医学研究生学报),2009,11(01):1-5.
　ZHANG Sheng-hang,LAN Xiao-peng,Yi Xun-Fei.Colon cancer subcutaneously inoculated in nude mice inhibited by siRNA to DcR3[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(01):1-5.
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靶向DcR3的siRNA抑制人结肠癌 裸鼠皮下移植瘤的实验研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第11卷
期数:
2009年01期
页码:
1-5
栏目:
出版日期:
2009-01-20

文章信息/Info

Title:
Colon cancer subcutaneously inoculated in nude mice inhibited by siRNA to DcR3
文章编号:
1672-271X(2009)01-0001-05
作者:
张胜行; 兰小鹏; 易浔飞
南京军区福州总医院检验科、全军检验医学研究所，福建福州 350025
Author(s):
ZHANG Sheng-hang; LAN Xiao-peng; Yi Xun-Fei
People's Liberation Army Center of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025,Fujian，China
关键词:
结肠癌; 基因治疗; SW480细胞; DcR3; siRNA; 裸鼠
Keywords:
Colorectal carcinoma;  Gene therapy;  SW480 cell;  DcR3;  SiRNA;  Nnude mice
分类号:
R730.231
DOI:
-
文献标志码:
A
摘要:
目的 以脂质体为载体，探讨靶向诱骗受体3(decoy receptor 3, DcR3)的siRNA 对人结肠癌SW480细胞裸鼠皮下移植瘤生长的抑制作用。方法 裸鼠背部皮下种植结肠癌SW480细胞，建立结肠癌SW480细胞裸鼠皮下移植瘤模型。针对靶标DcR3基因，利用Dharmacon公司软件设计并合成siRNA 序列，通过瘤体局部多点注射脂质体与DcR3 siRNA混合物，将DcR3 siRNA转染到裸鼠背部皮下移植瘤内。同时设立阴性对照组和空白对照组。观察肿瘤治疗前后体积变化，评价DcR3 siRNA对肿瘤的抑制作用；比较肿瘤治疗前后的细胞形态学改变；免疫组织化学及RT-PCR检测DcR3基因表达；原位细胞凋亡检测肿瘤的凋亡情况。结果 治疗组肿瘤体积明显小于对照组，肿瘤组织内坏死面积增大,细胞凋亡率显著增高, DcR3蛋白表达水平降低。治疗过程中裸鼠生长良好,无明显毒性反应。结论 脂质体介导的DcR3 siRNA对裸鼠皮下结肠癌SW480细胞移植瘤有明显抑制、杀伤作用，细胞凋亡是DcR3 siRNA所致肿瘤细胞死亡的重要形式。
Abstract:
Objective To investigate the effect of DcR3-siRNA mediated by liposome vector in inhibiting the growth of human cultured cancer cell SW480 subcutaneously inoculated in nude mice. Methods Athymic mice were subcutaneously transplanted with human colon carcinoma SW480 cells to establish a transplanted tumor model. To directly aim at target gene—DcR3, siRNA was synthesized by Dharmacon company and transfected into tumors subcutaneously transplanted in athymic mice. The negative and blank controls were established as well. The inhibitive effect of DcR3 siRNA to tumor was estimated. The changes on morphocytology and tumor volume were compared before and after therapy. The genetic expression of DcR3 was detected by immunohistochemistry and reverse transcriptase polymerase chain reaction. The tumor apoptosis was detected by TUMEL method. Results DcR3-siRNA can inhibit the growth of human colon cancer cell SW480. The expression of DcR3-mRNA in human cultured cancer cell SW480 was decreased significantly in the gene transfected group compared to that of control group. Conclusion DcR3 siRNA mediated by liposome distinctly inhibits the growth or kills SW480 cells subcutaneously transplanted in athymic mice.

参考文献/References:

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备注/Memo

备注/Memo:
福建省科技厅国际合作重点项目(2004I012)
更新日期/Last Update: 2013-11-20