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[1]何国军,黄 明,卢少军,等.5-HT2A受体对延髓面神经后核内侧区 呼吸节律的调控作用[J].医学研究与战创伤救治(原医学研究生学报),2009,11(01):6-９，１３.
　HE Guo-jun,HUANG Ming,LU Shao-jun,et al.Role of 5-HT2A receptors in regulation of respiratory rhythm in medial region of nucleus retrofacialis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(01):6-９，１３.
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5-HT2A受体对延髓面神经后核内侧区 呼吸节律的调控作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第11卷
期数:
2009年01期
页码:
6-９，１３
栏目:
出版日期:
2009-01-20

文章信息/Info

Title:
Role of 5-HT2A receptors in regulation of respiratory rhythm in medial region of nucleus retrofacialis
文章编号:
1672-271X(2009)01-0006-05
作者:
何国军1; 黄 明2; 卢少军1; 赖福生1; 吴中海3
1.解放军第454医院神经科，江苏南京 210002；2.空军雷达学院宜昌训练大队卫生队，湖北宜昌 443000；3.南方医科大学基础医学院生理教研室，广东广州 510515
Author(s):
HE Guo-jun1; HUANG Ming2; LU Shao-jun1;  LAI Fu-sheng1;  WU Zhong-hai3
1. Department of Neurology,the 454th Hospital of PLA, Nanjing 210002,Jiangsu,China; 2. Department of Health Team in Yichang Instruction Group, Air-Force Radar College, Yichang 443000,Hubei,China; 3. Department of Physiology, Southern Medical University, Guangzhou 510515,Guangdong, China
关键词:
呼吸中枢; 延髓; 面神经后核; 内侧区;  5-HT2A;  呼吸节律
Keywords:
Respiratory centre;  Medulla;  Nucleus retrofacialis;  medial region;  5-HT2A;  Respiratory rhythm
分类号:
R332
DOI:
-
文献标志码:
A
摘要:
目的 探讨5-HT2A受体在延髓面神经后核内侧区(the medial region of nucleus retrofac- ialis，mNRF)对呼吸节律调控的作用。方法 仿Suzue方法制作新生大鼠含有舌下神经根及mNRF的离体延髓脑片标本，以吸附电极记录舌下神经根呼吸节律性放电活动(the respiratory rhythmic discharge activity, RRDA)并作为呼吸活动的指标，采用全细胞膜片钳记录模式在mNRF同步记录呼吸神经元。分别观察1-(2, 5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI)、ketanserine对mNRF呼吸起步神经元及RRDA的影响。结果 DOI可显著使呼吸周期缩短、放电峰值增加，ketanserine使呼吸周期延长、放电峰值降低；DOI可使Cd2+非敏感性呼吸起步神经元发放幅度、时间、spike的频率显著增加，ketanserine则使其显著减少；voltage steps(电压阶跃) 和ramps(斜坡电压法)表明ketanserine可抑制Cd2+非敏感性呼吸起步神经元的短暂性和持久性的钠电流。结论 5-HT2A受体兴奋对RRDA、Cd2+非敏感性呼吸起步神经元具有兴奋性作用，是通过调节钠电流而起作用的。
Abstract:
Objective To explore the role of 5-HT2A receptors in regulation of respiratory rhythm in the medial region of nucleus retrofacialis (mNRF). Methods Medullary slices of neonatal SD rats, including hypoglossal nerve (Ⅻn) and mNRF,were made according to Suzue's method. Simultaneous recording of the Ⅻn respiratory rhythmic activity (RRDA) with suction electrode and the respiratory neuronal discharge were performed with whole cell patch in the mNRF on the brainstem slice in vitro. The effect of 1-(2, 5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI)and ketanserine on the respiratory pacemaker neurons and respiratory rhythm in the mNRF were observed. Results DOI significantly decreased respiratory cycle (RC) and increased peak value of discharge; Ketanserine significantly increased RC and decreased its peak value of discharge. Meanwhile, DOI significantly increased burst amplitude, durations and spike frequency of the respiratory cadmium-insensitive pacemaker neurons and ketanserine decreased them. Voltage steps and ramps showed that ketanserine inhibited both the transient and persistent sodium current of cadmium-insensitive respiratory pacemaker neurons.Conclusion 5-HT2A receptors has an excitatory effect on the respiratory rhythm and the cadmium-insensitive respiratory pacemaker neurons through modulating sodium currents.

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备注/Memo

备注/Memo:
国家自然科学基金(30570670)；广东省自然科学基金(5004714)
更新日期/Last Update: 2013-11-20