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[1]王建东,王绪林,董迎春,等.TaqMan探针检测结直肠癌血浆中微量 甲基化EphA7基因[J].医学研究与战创伤救治(原医学研究生学报),2009,11(02):97-99,130.
 WANG Jian-dong,WANG Xu-lin,DONG Ying-chun,et al.Microamount of hypermethylated EphA7 detected in plasma of colorectal cancer with a TaqMan probe[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(02):97-99,130.
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TaqMan探针检测结直肠癌血浆中微量 甲基化EphA7基因()

《医学研究与战创伤救治》(原医学研究生学报)[ISSN:1672-271X/CN:32-1713/R]

卷:
第11卷
期数:
2009年02期
页码:
97-99,130
栏目:
出版日期:
2009-03-20

文章信息/Info

Title:
Microamount of hypermethylated EphA7 detected in plasma of colorectal cancer with a TaqMan probe
文章编号:
1672-271X(2009)02-0097-04
作者:
王建东1王绪林2董迎春1李国立2鲍 杨2马恒辉1周晓军1
南京军区南京总医院,1.病理科;2.普外科,江苏南京 210002
Author(s):
WANG Jian-dong1 WANG Xu-lin2 DONG Ying-chun1 LI Guo-li2 BAO Yang2 MA Heng-hui1 ZHOU Xiao-jun1
1.Department of Pathology;2.Department of Surgery,Nanjing General Hospital of Nanjing Military Command, PLA,Nanjing 210002,Jiangsu,China
关键词:
光标记探针实时PCR血浆甲基化EphA7
Keywords:
Fluorescent TaqMan probe Real-time PCR Plasma Hyperme thylation EphA7
分类号:
R735.3+7
DOI:
-
文献标志码:
A
摘要:
目的 从结直肠癌症患者血浆中分离微量来源于肿瘤细胞的游离DNA,检测甲基化EphA7作为肿瘤标志。方法 提取血浆中微量游离DNA,并以亚硫酸氢钠修饰DNA。根据修饰后的DNA序列,设计EphA7甲基化特异性引物和探针,利用Real Time PCR仪扩增甲基化EphA7基因。结果 从大肠癌患者血浆中检出微量甲基化EphA7基因。结论 应用荧光标记TaqMan 探针Real Time PCR可以检测出结直肠癌患者血浆中微量游离DNA中甲基化EphA7基因,血浆甲基化EphA7可能作为一种新的肿瘤标志物。
Abstract:
Objective To detect hypermethylated EphA7 in plasma DNA released from colorectal cancer cells as a tumor marker.Methods The free DNA in plasma was extracted using a QIAamp DNA Blood Mini kit and treated with sodium bisolfite. The Real time PCR primer sets and fluorescence labeled probe for detection of hypermethylated EphA7 were designed according to the sodium bisulfte converted DNA sequence. Hypermethylated EphA7 DNA was detected using an ABI PRISM 7000 sequence detection system.Results The hypermethylated EphA7 gene was detected in plasma of colorectal cancer.Conclusion Real-time PCR with a fluorescence labeled TaqMan probe can detect hypermethylated EphA7 gene from plasma of colorectal cancer, and hypermethylated EphA7 in plasma maybe used as a new tumor marker.

参考文献/References:

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备注/Memo

备注/Memo:
国家基础研究973项目(2006, CB 705707)
更新日期/Last Update: 2013-11-20