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[1]王达理,楼建英,张 伟,等.维拉帕米对心肌细胞的保护与毒性作用[J].医学研究与战创伤救治(原医学研究生学报),2010,12(01):21-23.
　WANG Da-li,LOU Jian-yin,ZHANG Wei,et al.The protection and cytotoxity of verapamil on heart cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(01):21-23.
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维拉帕米对心肌细胞的保护与毒性作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年01期
页码:
21-23
栏目:
出版日期:
2010-01-20

文章信息/Info

Title:
The protection and cytotoxity of verapamil on heart cells
文章编号:
1672-271X(2010)01-0021-03
作者:
王达理; 楼建英; 张 伟; 韩 燕; 陈金安
210002江苏南京，解放军454医院心内科
Author(s):
WANG Da-li; LOU Jian-yin; ZHANG Wei; HAN Yan; CHEN Jin-an.
Department of Cardiology，454 Hospital of PLA，Nanjing,Jiangsu 210002，China
关键词:
心肌细胞; 维拉帕米; 保护; 细胞毒性; 乳酸脱氢酶; 肌酸磷酸激酶
Keywords:
heart cell; verapamil; protection;  cytotoxity;  lactic dehydrogenase(LDH);  creatine kinase(CK)
分类号:
R
DOI:
-
文献标志码:
A
摘要:
目的 研究维拉帕米(verapamil, Ver)对心肌细胞的保护作用与毒性。方法 体外培养心肌细胞，测定培养液中乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)的活性;观察不同浓度维拉帕米对心肌细胞的毒性作用及对缺氧再复氧模拟“缺血再灌注”细胞的保护作用。结果 连续培养96 h的结果与正常对照组相比，Ver 10.0 mg/L以上各浓度组存活细胞代谢比色值A540减少、LDH活性增高具有明显的意义；比色值A540与细胞释放的LDH、CK活性呈高度相关(r＝-0.986、-0.989, P<0.01)；维拉帕米可使缺血再灌注损伤的心肌细胞A540显著升高、CK显著降低。结论 维拉帕米在缺氧再复氧时有明显的细胞保护作用，但是高浓度特别是超过10 mg/L时的直接细胞毒性作用值得关注。
Abstract:
Objective To study the protection and cytotoxity of verapamile on heart cells. Methods Heart cells were cultured. The activity of lactate dehydrogenase and creatine phosphokinase in culture medium was detected. The toxicity of verapamil to heart cells in different concerntration and protective role of verapamil to heart cells during hypoxia-reoxygennation were observed by MTT assay and testing of CK and LDH activity. Results By continuously incubation for 96 h, the value of A540 decreased and LDH activity increased in cultured heart cells with the concentration of verapamil at 10.0 mg/L or higher compared with control cells. A540 response curves were highly correlated with CK activity ( r =-0.986， P＜0.01 ) and LDH activity ( r =-0.989， P＜0.01). The value of A540 significantly increase and CK activity obviously reduce in heart cells simulative ischemia and reperfusion after treatment with verapamil. Conclusion Verapamil protects heart cells in simulative ischemia and reperfusion, but the cytotoxicity is worth paying close attention when verapamil concentration is over 10 mg/L.

参考文献/References:

[1] 朱 平，程源恩，黄 立，等.异搏定加钾停搏液对心肌保护作用的实验研究[J].中华医学杂志, 1988,68(6):316-318.
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[9] 乔志卿, 卜 军, 丁 嵩, 等. 冠状动脉内应用维拉帕米对急性心肌梗死急诊介入治疗后冠状动脉灌注、心肌灌注和临床预后的影响[J]. 中国介入心脏病学杂志, 2009,17(4):185-190.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-01-20