|本期目录/Table of Contents|

[1]杨春宝,丁江华,郑章清.染料木黄酮脂质体的制备及其对肿瘤细胞体外增殖的抑制作用[J].医学研究与战创伤救治(原医学研究生学报),2010,12(01):33-35.
　YANG Chun-bao,DING Jiang-hua,ZHENG Zhang-qing..Preparation of Genistein liposomes and its effect on the proliferation of tumor cells in vitro[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(01):33-35.
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染料木黄酮脂质体的制备及其对肿瘤细胞体外增殖的抑制作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年01期
页码:
33-35
栏目:
出版日期:
2010-01-20

文章信息/Info

Title:
Preparation of Genistein liposomes and its effect on the proliferation of tumor cells in vitro
文章编号:
1672-271X(2010)01-0033-03
作者:
杨春宝1; 丁江华2; 郑章清1
332000江西九江，南京军区庐山疗养院,1.药剂科，2.肿瘤科
Author(s):
YANG Chun-bao1; DING Jiang-hua2; ZHENG Zhang-qing1.
1.Department of Pharmacy, 2.Department of Neoplasm,Lushan Sanatorium,Nanjing Military Command,Jiujiang,Jiangxi 332000,China
关键词:
肿瘤细胞; 染料木黄酮脂质体; IC50
Keywords:
tumor cells;  Genisteim liposomes;  IC50
分类号:
R647；R622+.1
DOI:
-
文献标志码:
A
摘要:
目的 研究染料木黄酮脂质体的制备方法及其对肿瘤细胞体外增殖的影响。方法 采用超声薄膜法制备染料木黄酮脂质体；活细胞计数法和MTT法观察染料木黄酮脂质体对肿瘤细胞的增殖抑制作用。结果 染料木黄酮脂质体的平均粒径为136.7 nm，剂量依赖性抑制A549和HepG2细胞的增殖，IC50分别为10.46、12.34 μmol/L。结论 染料木黄酮脂质体对A549和HepG2肿瘤细胞有明显的增殖抑制作用，且作用强于游离染料木黄酮。
Abstract:
Objective To study the method for preparation of liposomes embedding Genistein and the effect of Genistein liposomes on the proliferation of tumor cells in vitro. Methods The Genisteim liposomes were prepared by using a ultrasonic film method. The effect of Genisteim liposomes on proliferation of tumor cells was determined by the growth curves of tumor cells using live cell calculation and MTT method. Results The average diameter of Genistein liposomes was 136.7 nm. The liposomes inhibited the growth of A549 and HepG2 cells in vitro in a dose-dependent manner and the IC50 of Genistein liposomes on A549 and HepG2 cells were 10.46×10-6 mol/L and 12.34×10-6 mol/L respectively. Conclusion The Genisteim liposomes inhibited the proliferation of A549 and HepG2 cells. It is more effective than free Genisteim.

参考文献/References:

[1] 蒋天智，刘少友.新型长循环紫杉醇脂质体的制备及其细胞毒性[J].中国组织工程研究与临床康复,2007，11(26):5113-5116.
[2] Chatzinikolaou G, Nikitovic D, Stathopoulos EN, et al. Protein tyrosine kinase and estrogen receptor-dependent pathways regulate the synthesis and distribution of glycosaminoglycans/proteoglycans produced by two human colon cancer cell lines[J]. Anticancer Res, 2007,27(6):4101-4106.
[3] Schmidt F, Knobbe CB, Frank B, et al. The topoisomerase Ⅱinhibitor, genistein, induces G2/M arrest and apoptosis in human malignant glioma cell lines[J]. Oncol Rep, 2008,19(4):1061-1066.
[4] Wiegand H, wagner AE, Boesch-Saadatmandi C, et al. Effect of Dietary Genistein on Phase II and Antioxidant Enzymes in Rat Liver[J]. Cancer Genomics Proteomics, 2009,6(1): 85-92.
[5] Ford JA,Clark SG,Walters EM,et al.Estrogenic effects of genistein on reproductive tissues of ovariectomized gilts[J].J Anim Sci, 2006, 84(4): 834-842.
[6] Cave NJ, Backus RC，Marks SL,et al.Modulation of innate and acquired immunity by an estrogenic dose of genistein in gonadectomized cats[J].Vet Immunol Immunopathol, 2007, 117(1-2):42-54.
[7] Jin Y, Wu H, Cohen EM, et al.Genistein and daidzein induce neurotoxicity at high concentrations in primary rat neuronal cultures[J].J Biomed Sci,2007,14(2): 275-284.
[8] Klein CB,King AA.Genistein genotoxicity: critical considerations of in vitro exposure dose[J].Toxicol Appl Pharmacol,2007,224(1):1-11.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-01-20