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[1]肖可为,辛晓峰,刘雪梅,等.哮喘患者检测痰中基质金属蛋白酶的临床意义[J].医学研究与战创伤救治(原医学研究生学报),2010,12(02):112-114.
　XIAO Ke-wei,XIN Xiao-feng,LIU Xue-mei,et al.Clinical significance of test of metalloproteinase in sputum from asthma patients[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(02):112-114.
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哮喘患者检测痰中基质金属蛋白酶的临床意义()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年02期
页码:
112-114
栏目:
出版日期:
2010-03-20

文章信息/Info

Title:
Clinical significance of test of metalloproteinase in sputum from asthma patients
文章编号:
1672-271X(2010)02-0112-03
作者:
肖可为1; 辛晓峰2; 刘雪梅3; 何宏伟1
1.210002 江苏南京,南京军区联勤部门诊部；2.210002 江苏南京，南京军区南京总医院呼吸科；3.210000 江苏南京，南京建邺区医院检验科
Author(s):
XIAO Ke-wei1 ;  XIN Xiao-feng2;  LIU Xue-mei3; HE Hong-wei1.
1.Outpatient Department of Combined Service Force, Nanjing Military Command, Nanjing,Jiangsu 210002, China; 2. Department of Pulmonary Diseases,Nanjing General Hospital of Nanjing Military Command，Nanjing, Jiangsu 210002, China; 3.Department of Detectio
关键词:
哮喘;  基质金属蛋白酶9;  基质金属蛋白酶抑制剂1; 气道炎症; 气流受限
Keywords:
asthma;  MMP-9; TIMP-1;  airway inflammation;  airflow limitation
分类号:
R256.12
DOI:
-
文献标志码:
A
摘要:
目的 探讨支气管哮喘(简称哮喘)患者诱导痰中基质金属蛋白酶9(MMP-9)和基质金属蛋白酶抑制剂1(TIMP-1)的浓度比值与气道炎症和气流受限的关系。方法 选择缓解期哮喘患者(哮喘组)和健康对照者(健康对照组)进行肺功能测定；用诱导痰检查方法对痰炎性细胞进行分类计数，并用酶联免疫吸附试验(ELISA)法测定诱导痰上清液中 MMP-9和TIMP-1浓度。结果 哮喘组和健康对照组诱导痰中MMP-9、TIMP-1浓度比较差异和MMP-9/TIMP-1比值比较差异有统计学意义。结论 哮喘组患者诱导痰中MMP-9/TIMP-1比值的失衡与气道炎症有关，从而造成气道重塑和气流受限，导致肺功能下降。
Abstract:
Objective To investigate the relationship between the levels of metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in induced sputum in patients with asthma and the airway inflammation and airflow limitation. Methods Sixteen patients with asthma in remission stages, and twelve normal control subjects were included in this study. Lung function was measured. Induced sputum was obtained and processed for cell differential count. The concentrations of MMP-9 and TIMP-1 in supernatant were assayed by enzyme-linked immunosorbent assay (ELISA). Results The difference of the concentration of MMP-9/TIMP-1 in induced sputum and the difference of the ratio of MMP-9/TIMP-1 in asthmatics and in normal control subjects were statistical analyzed. Conclusion The imbalance between MMP-9 and TIMP-1 in induced sputum in asthmatics is associated with airway inflammation and airflow limitation, which may play a role in the pathogenesis of extracellular matrix remodeling and airflow limitation.

参考文献/References:

[1]Demedts IK, Brusselle GG, Bracke KR, et al.Matrix metalloproteinases in asthma and COPD［J］. Curr Opin Pharmacol， 2005，5(6):257-263.
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[5］Matsmoto H,Niimi A,Takemura M,et al. Relationship of airway wall thickening to an imbalance between matrix metalloproteinase-9 and its inhibitor in asthma［J］. Thorax,2005,60(12):277-281.
[6］Lee KS, Jin SM, Lee H, et al. Imbalance between matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in toluene diisocyanate-induced asthma［J］. Clin Exp Allergy， 2004，34(5):276-284.
[7］Bosse M, Chakir J, Rouabhia M, et al. Serum matrix metalloproteinase-9:tissue inhibitor of metalloproteinase-1 ratio correlates with steroid responsiveness in moderate to severe asthma［J］. Am J Respir Crit Care Med，1999，159(6):596-602.
[8］Bergeron C, Page N, Barbeau B, et al. Interleukin-4 promotes airway remodeling in asthma: regulation of procollagen I (alpha1) gene by interleukin-4［J］. Chest， 2003，123(3 Suppl):424S.

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备注/Memo:
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更新日期/Last Update: 2010-03-20