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[1]李爱梅,林夏雯,贾 鹏,等.18F-氟代脱氧葡萄糖诱导MCF-7细胞株的凋亡研究 李爱梅，林夏雯，贾 鹏，郭万华[摘要][J].医学研究与战创伤救治(原医学研究生学报),2011,13(02):112-115.
　LI Ai-mei,LIN Xia-wen,JIA Peng,et al.Effect of 2-deoxy-2-[18F]fluoro-D-glucose on apoptosis of human MCF-7 cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2011,13(02):112-115.
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18F-氟代脱氧葡萄糖诱导MCF-7细胞株的凋亡研究 李爱梅，林夏雯，贾 鹏，郭万华 [摘要] ()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第13卷
期数:
2011年02期
页码:
112-115
栏目:
出版日期:
2011-03-20

文章信息/Info

Title:
Effect of 2-deoxy-2-[18F]fluoro-D-glucose on apoptosis of human MCF-7 cells
文章编号:
1672-271X(2011)02-0112-04
作者:
李爱梅; 林夏雯; 贾 鹏; 郭万华
210008 江苏南京，南京大学附属鼓楼医院核医学科
Author(s):
LI Ai-mei; LIN Xia-wen; JIA Peng; GUO Wan-hua
Nuclear Medicine Department of Nanjing Drum Tower Hospital,Nanjing,Jiangsu 210008,China
关键词:
18F-氟代脱氧葡萄糖; MCF-7细胞; 凋亡
Keywords:
18F-FDG;  MCF-7 cells;  apoptosis
分类号:
R730.55
DOI:
-
文献标志码:
A
摘要:
目的 研究18F-氟代脱氧葡萄糖(18F-FDG)诱导体外培养MCF-7乳腺癌细胞的凋亡情况。方法 不同剂量18F-FDG加入体外培养MCF-7乳腺癌细胞中共培养，以γ计数仪测定细胞摄取18F-FDG的量；用流式细胞仪测定细胞早期和晚期凋亡率；用TUNEL法测定细胞凋亡的数量。结果 ①相同数量级的MCF-7细胞对18F-FDG的摄取量与剂量增加呈线性;②流式细胞结果示:经12 h 18F-FDG孵育引起MCF-7的平均早期凋亡率为12.5%，平均晚期凋亡率为39.3%，总平均凋亡率为51.8%。经24 h 18F-FDG孵育引起MCF-7的平均早期凋亡率为10.4%，平均晚期凋亡率为42.1%，总平均凋亡率为52.5%; ③TUNEL显示:用凋亡指数(apoptotic index，AI)表示，与空白对照组AI(5.13±1.51)%相比，18F-FDG孵育12 h爬片的AI为(45.93±1.51)%，18F-FDG孵育24 h爬片的AI为(46.93±1.52)%。结论 一定剂量范围内，18F-FDG对MCF-7细胞诱导凋亡效果显著。
Abstract:
Objective To investigate effect of 2-deoxy-2-[18F]fluoro-D-glucose on apoptosis of human MCF-7 cells. Methods Different doses of 18F-FDG were co-cultured with MCF-7 in vitro. γ-ray high energy counting machine was used to count 18F-FDG uptaking in MCF-7 cells. The cell apoptosis percent was detected by flow cytometry. The number of apoptosis cells was counted by using a TUNEL kit. Results ①In vitro, uptaking 18F-FDG of the same number MCF-7 cells related with dose in line.②FCM displayed that after 18F-FDG incubating for 12h the early average apoptosis percent was 12.5%, the later average apoptosis percent was 39.3%, and total average apoptosis percent was 51.8%. After 18F-FDG incubating 24h, the early average apoptosis percent was 10.4%, the later average apoptosis percent was 42.1%,total average apoptosis percent was 52.5%.③TUNEL kit analysis showed that AI was (45.93±1.51)% after 18F-FDG incubating 12h, AI was (46.93±1.52)% after 18F-FDG incubating 24h, compared with control of AI was (5.13±1.51)%. Conclusion Apoptosis of human MCF-7 cells was induced by moderate 18F-FDG dose.

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备注/Memo

备注/Memo:
南京市医学重点科技发展项目(ZKX07009)
更新日期/Last Update: 2011-03-20