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[1]吴 豫,唐古生,赵伟国,等.心肌肌钙蛋白I自身抗体间接酶联免疫吸附分析法的建立[J].医学研究与战创伤救治(原医学研究生学报),2011,13(03):211.
　WU Yu,TANG Gu-Sheng,ZHAO Wei-Guo,et al.Establishment of an indirect ELISA method with recombination cTnI-C fusion protein for detection of cTnI autoantibody[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2011,13(03):211.
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心肌肌钙蛋白I自身抗体间接酶联免疫吸附分析法的建立()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第13卷
期数:
2011年03期
页码:
211
栏目:
出版日期:
2011-05-20

文章信息/Info

Title:
Establishment of an indirect ELISA method with recombination cTnI-C fusion protein for detection of cTnI autoantibody
文章编号:
1672-271X(2011)03-0211-04
作者:
吴 豫1; 唐古生2; 赵伟国3; 沈 茜2
1.330002 江西南昌，解放军94医院检验病理科；2.200433 上海，第二军医大学附属长海医院实验诊断科；3.200433 上海，上海博阳生物科技有限公司
Author(s):
WU Yu1; TANG Gu-Sheng2; ZHAO Wei-Guo3; SHEN Qian2.
1.Department of Laboratory Medicine, 94 Hospital of PLA, Nanchang,Jiangxi 330002,China;2.Department of Laboratory Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;3.Beyond Diagnostics Inc.，shanghai 200433,China
关键词:
心肌梗死; 重组人心肌肌钙蛋白I-C; 肌钙蛋白C; 人循环心肌肌钙蛋白I自身抗体; 间酶联免疫吸附分析法
Keywords:
myocardial infarction; cardial troponin I-C; troponin C; cTnI autoantibody; indirect ELISA
分类号:
R542.22
DOI:
-
文献标志码:
A
摘要:
目的 建立以重组心肌肌钙蛋白I-C(cTnI-C)融合蛋白为包被抗原的间接酶联免疫吸附分析法(ELISA)，检测人血循环中的抗cTnI自身抗体，为判断心肌损伤的预后提供依据。方法 采用自行构建表达的cTnI-C融合蛋白作为包被抗原，以棋盘法优化实验条件，建立检测人血清cTnI自身抗体的间接ELISA方法。采用建立的间接ELISA法在121例急性心肌梗死(AMI)中筛查抗cTnI自身抗体，并用蛋白印迹法(WB)进行确证。结果 121例AMI血清抗cTnI抗体的阳性率为10.74%，ELISA试验的灵敏度100%，特异性95.37%，Kappa值为0.815。结论 以cTnI-C融合蛋白作为包被蛋白建立的间接ELISA法具有良好的敏感度、特异性，可用于人血清抗cTnI自身抗体的筛查。
Abstract:
Objective To establish an indirect ELISA using cTnI-C recombinant protein as coating antigen in detecting cTnI autoantibody, and to provide with a method for detection of cTnI autoantibody in blood circulation and the prognostic test for myocardial damage. Methods cTnI-C recombinant protein was used as coating protein to establish indirect ELISA through checkerboard method. Screening of cTnI serum autoantibody in 121 AMI patients was done by indirect ELISA and was confirmed by western blotting. Results cTnI autoantibody was positively detected in 10.74% of the 121 AMI patients. The sensitivity of detecting cTnI autoantibody with this recombinant protein was 100% and specificity was 95%. Kappa value was 0.82. Conclusion The cTnI-C fusion protein can be used to indirect ELISA in detecting cTnI autoantibody.

参考文献/References:

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[4]丁雪燕，罗助荣.急性冠脉综合征血清脑钠肽水平及阿托伐他汀对其影响[J].东南国防医药，2009，11(1):37-39.
[5]Okazaki T，Tanaka Y，Nishio R， et al.Autoantibody against cardiac troponin I are responsible for dilated cardiomyopathy in PD-1-deficient mice[J].Nat Med，2003，9(12):1477-1483.
[6]吴 豫，赵伟国，沈 茜.循环心肌肌钙蛋白I自身抗体的检测及临床意义[J].中华检验医学杂志，2008，31(7):801-806.
[7]Mockel M，Heller G Jr，Berg K，et al.The acute coronary syndrome diagnosis and prognosis evaluation by troponin I is influenced by the test system affinity to different troponin complexes[J].Clin Chem Acta，2000，293(1-2):139-155.
[8]Datta P，Foster K，Dasgupta A.Comparison of immunoreactivity of five human cardiac troponin I assay toward free and complexed forms of the antige:implications for assay discordance[J].Clin Chem，1999，45(12):2266-2269.

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备注/Memo

备注/Memo:
南京军区重点课题资助(10Z016)
更新日期/Last Update: 2011-05-20