|本期目录/Table of Contents|

[1]杨 燕,孙长贵,陈 坚,等.用改良Hodge试验检测产碳青霉烯酶肠杆菌科细菌[J].医学研究与战创伤救治(原医学研究生学报),2011,13(03):223-225.
　YANG Yan,SUN Chang-gui,CHEN Jian,et al.Detection of carbapenemase in isolates of Enterobacteriaceae by the modified Hodge test[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2011,13(03):223-225.
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用改良Hodge试验检测产碳青霉烯酶肠杆菌科细菌()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第13卷
期数:
2011年03期
页码:
223-225
栏目:
出版日期:
2011-05-20

文章信息/Info

Title:
Detection of carbapenemase in isolates of Enterobacteriaceae by the modified Hodge test
文章编号:
1672-271X(2011)03-0223-03
作者:
杨 燕; 孙长贵; 陈 坚; 熊 敏; 成 军
310013 浙江杭州，解放军117医院检验科
Author(s):
YANG Yan; SUN Chang-gui; CHEN Jian; XIONG Min; CHENG Jun
Department of Clinical Laboratory，117 Hospital of PLA，Hangzhou,Zhejiang 310013，China
关键词:
肠杆菌科; 碳青霉烯酶; 抗生素; 敏感性
Keywords:
Enterobacteriaceae;  carbapenemase;  antibiotic;  sensitivity
分类号:
R378.2
DOI:
-
文献标志码:
A
摘要:
目的 了解分离的肠杆菌科细菌产碳青霉烯酶情况，为临床正确选择抗菌药物提供依据。方法 采用K-B纸片扩散法检测并筛选厄他培南和美罗培南，以抑菌环直径分别为19~21 mm和16~21 mm的菌株作为待测菌，进行改良的Hodge试验。 结果 390株肠杆菌科细菌中筛选出55株可疑产碳青霉烯酶菌株，对可疑菌株进行改良的Hodge试验，阳性率21.82%(12/55)。结论 碳青霉烯类抗生素敏感折点附近的肠杆菌科细菌有近1/5菌株产碳青霉烯酶，临床微生物学实验室应执行改良Hodge试验，以确保药敏试验结果的准确性。
Abstract:
Objective To investigate carbapenemase production in isolates of Enterobacteriaceae isolated from our hospital and provide a correct choice of antibiotic for the clinician. Methods Antibiotic susceptibility test was performed by K-B method. Strains showing zones of inhibition 19-21 mm with Ertapenem or 16-21 mm with Meropenem were screened as suspicious carbapenemase producing ones and tested for carbapenemase by the modified Hodge test. Results 55 of 390 strains of Enterobacteriaceae were suspected of possessing carbapenemases, which revealed 21.82% positive rate in the modified Hodge test. Conclusion Nearly one-fifth of strains of Enterobacteriaceae whose carbapenems zones of inhibition were nearby susceptible break point generated carbapenemase. The modified Hodge test should be done to ensure the accuracy of the sensitivity test.

参考文献/References:

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[3]Schwaber MJ, Klarfeld-Lidji S, Navon-Venezia S, et al.Predictors of carbapenem-resistant Klebsiella pneumoniae acquisition among hospitalized adults and effect of acquisition on mortality[J].Antimicrob Agents Chemother, 2008,52(3):1028-1033.
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[8]夏 云,曹 何,张晓恒,等.革兰阴性杆菌对碳青霉烯类抗生素耐药率的变化及用量的关系 [J].临床检验杂志, 2010,28(3):237-238.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-05-20