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[1]唐庆林,宫秀群,冯根宝,等.肠易激综合征大鼠内脏高敏感性与脑肠互动的研究[J].医学研究与战创伤救治(原医学研究生学报),2012,14(01):1-4.
　TANG Qing-lin,GONG Xiu-qun,FENG Gen-bao,et al.The investigation of the relation between visceral hypersensitivity and brain-gut interaction in the rat model with irritable bowel syndrome[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(01):1-4.
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肠易激综合征大鼠内脏高敏感性与脑肠互动的研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年01期
页码:
1-4
栏目:
出版日期:
2012-01-20

文章信息/Info

Title:
The investigation of the relation between visceral hypersensitivity and brain-gut interaction in the rat model with irritable bowel syndrome
文章编号:
1672-271X(2012)01-0001-04
作者:
唐庆林1; 宫秀群2; 冯根宝2; 李雪飞3; 汪芳裕1
210002 江苏南京，第二军医大学南京临床医学院(南京军区南京总医院)，1.消化内科，2.神经内科，3.麻醉科
Author(s):
TANG Qing-lin1; GONG Xiu-qun2; FENG Gen-bao2; LI Xue-fei3; WANG Fang-yu1.
1.Department of Gastroenterology,2.Department of Neurology,3.Department of Anesthesiology,Nanjing School of Clinical Medicine,the Second Military Medical University/Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China
关键词:
肠易激综合征; 内脏敏感性; c-fos
Keywords:
irritable bowel syndrome;  visceral sensitivity;  c-fos
分类号:
R574
DOI:
-
文献标志码:
A
摘要:
目的 研究腹泻型肠易激综合征大鼠模型内脏敏感性的改变与c-fos在结肠及中枢神经系统异常表达的关系。方法 采用乙酸灌肠法造成腹泻型肠易激综合征动物模型(A组，n=12)和对照组(B组，n=12)，造模后通过腹部回撤反应评分评价内脏敏感性；用印度墨汁肠道染色法观察小肠蠕动，以墨汁在小肠中移行的距离占整段小肠长度的百分比来观察小肠推进蠕动的变化；应用免疫组织化学染色及计算机图像分析系统半定量分析结肠及中枢神经系统c-fos表达。结果 该模型符合腹泻型肠易激综合征特征，A组腹部回撤反应评分、结肠及中枢神经系统c-fos表达高于B组，两组差异有统计学意义(P＜0.05)。结论 乙酸灌肠法造成腹泻型肠易激综合征大鼠模型内脏高敏感性，c-fos的高表达参与大鼠内脏高敏感的异常调节。
Abstract:
Objective To study the interaction of the visceral hypersensitivity and the abnormal expression of c-fos in the colon and central nervous system in the rat model of diarrhea-predominant irritable bowel syndrome.Methods The rat model of diarrhea-predominant irritable bowel syndrome was induced by the intracolonic administration of acetic acid.The visceral sensitivity was evaluated by the score of abdominal withdrawal reflexes in the model group (group A,n=12) and the control group (group B,n=12) .A colored marker (indian ink) was employed for the measurement of small intestinal transit.The expression of c-fos in the colon and central nervous system was detected by an immunohistochemical staining and was analyzed semi-quantitatively by computerized color image analyzer.Results The character of this model matched the character of diarrhea-predominant irritable bowel syndrome.The score of abdominal withdrawal reflexes in group A was significantly higher than that of group B (P＜0.05).Also, the expression of c-fos in the colon and central nervous system in group A was greater than that of group B (P＜0.05).Conclusion The rat model of diarrhea-predominant irritable bowel syndrome which was established the intracolonic administration of acetic acid shows the character of visceral hypersensitivity.And the hyperexpression of c-fos in the colon and central nervous system is probably involved in the pathophysiology of visceral hypersensitivity.

参考文献/References:

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[9]Ait-Belgnaoui A,Eutamene H,Houdeau E,et al.Lactobacillus farciminis treatment attenuates stress-induced overexpression of fos protein in spinal and supraspinal sites after colorectal distension in rats[J].Neurogastroenterol Motil,2009,21(5):567-573.
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备注/Memo

备注/Memo:
国家自然科学基金(81070289)；南京军区南京总医院科研基金(Z208019)
更新日期/Last Update: 2012-01-20