|本期目录/Table of Contents|

[1]孙茂华,孟祥升,夏兴霞,等.大肠杆菌O157:H7实验感染动物排菌动态检测[J].医学研究与战创伤救治(原医学研究生学报),2012,14(01):5-8.
　SUN Mao-hua,MENG Xiang-sheng,XIA Xing-xia,et al.Detection of the faeces of animals inoculated with E.coli O157:H7[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(01):5-8.
点击复制

大肠杆菌O157:H7实验感染动物排菌动态检测()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年01期
页码:
5-8
栏目:
出版日期:
2012-01-20

文章信息/Info

Title:
Detection of the faeces of animals inoculated with E.coli O157:H7
文章编号:
1672-271X(2012)01-0005-04
作者:
孙茂华1; 孟祥升2; 夏兴霞2; 赵攀登2; 张雪寒2; 何孔旺2; 王永山2
1.210002 江苏南京,南京军区联勤部门诊部；2.210014 江苏南京，江苏省农业科学院兽医研究所
Author(s):
SUN Mao-hua1; MENG Xiang-sheng2; XIA Xing-xia2; ZHAO Pan-deng2; ZHANG Xue-han2; HE Kong-wang2; WANG Yong-shan2.
1.Outpatient Clinic,Joint Logistics Department of Nanjing Military Area Command,Nanjing,Jiangsu 210002,China; 2.Institute of Veterinary Medicine,Jiangsu Academy of Agricutural Sciences/ National Center for Engineering Research of Veterinary Bio-products,Nanjing,Jiangsu 210014,China
关键词:
胶体金免疫层析检测试纸条; 肠出血性大肠杆菌O157:H7; 牛; 鼠; 粪便; 检测
Keywords:
gold immunochromatographic strip;  E.coli O157:H7;  cattle;  mouse;  faeces;  detection
分类号:
R378.2
DOI:
-
文献标志码:
A
摘要:
目的 检验大肠杆菌O157:H7胶体金免疫层析检测试纸条在感染动物排菌中的应用效果。方法 将牛和小鼠采用灌胃攻毒方式实验感染大肠杆菌O157:H7，用大肠杆菌O157:H7胶体金免疫层析检测试纸条与细菌鉴别培养基分别培养检测感染动物粪便中的O157:H7的排菌量和持续时间。结果 牛在感染大肠杆菌O157:H7后的第2天开始从粪便排菌，第4~6天达到峰值，排菌时间可持续28 d；小鼠在感染O157:H7后的第4小时开始从粪便排菌，第6小时达到峰值，排菌时间可持续15 d。结论 大肠杆菌O157:H7胶体金免疫层析检测试纸条与细菌鉴别培养基培养计数的检测结果一致，试纸条更简便、快捷、直观，在1~5 min内可得出检测结果。
Abstract:
Objective To investigate the application of the gold immunochromatographic strip for the detection of animals inoculated with E.coli O157:H7.Methods The cattle and mice were inoculated intragastrically with E.coli O157:H7,and their faeces were detected by using the gold immunochromatographic strip and counting colony.Results E.coli O157:H7 in faeces could be detected on day 2 after inoculation in inoculated cattle by using immunochromatographic strip of E.coli O157:H7 and the method of plate culture count with differential medium of E.coli O157:H7 and the peak levels of O157:H7 in faeces were attained during the forth to sixth day after inoculation and then it dropped down gradually up to day 28 following inoculation.The O157:H7 in faeces could be detected on hour 4 after inoculation in inoculated mice by using IC strip of E.coli O157:H7 and the method of plate culture count with differential medium of E.coli O157:H7 and the peak levels of O157:H7 in faeces were attained during the sixth hour after inoculation and then it dropped down gradually up to day 15 following inoculation.Conclusion The gold immunochromatographic strip for the detection of E.coli O157:H7 is convenient and rapid for the clinical diagnosis and field investigation of E.coli O157:H7 infection or/and contamination.

参考文献/References:

[1]Tarr PI.Escherichia coli O157 :H7:clinical,diagnostic and epidemiological aspects of human infection [J].Clin Infect Dis,1995,20(1):1-10.
[2]WHO.Enterohaemorrhagic Escherichia coil infection Japan[J].Wkly Epid Rec，1996，30:229.
[3]李洪卫，景怀琦，逢 波，等.徐州市2000年肠出血性大肠埃希菌0157:H7感染性腹泻的调查[J].中华流行病学杂志，2004，23(2):119-122.
[4]倪大新，汪 华，顾 玲，等.江苏省1999年大肠埃希菌0157:H7宿主动物带菌情况调查[J].中华流行病学杂志，2002，3(2):102-104.
[5]Hancock D,Besser T,Lejeune J.The control of VTEC in the animal reservoir [J].Int J Food Microbiol,2001,66(1-2):71-78.
[6]Ogden ID,Hepburn NF,MacRae M,et al.Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp [J].Lett Appl Microbiol,2002,34(2):100-104.
[7]Meyer-Broseta S，Bastian SN，Arne PD，et al.Review of epidemiological surveyson the prevalence of contamination of healthy cattle with Escherichia coil serogroup O157:H7[J].lnternational Journal of Hygiene and Environmental Health，2001，203:347-361.
[8]刘 洁，夏兴霞，王永山，等.大肠杆菌0157:H7抗体胶体金免疫层析检测试纸条的研制[J].中国预防兽医学报，2010，32(5):375-378.
[9]夏兴霞，刘 洁，王永山，等.动物源性人兽共患细菌病防控生物新制剂的研究I.分泌抗大肠杆菌0157:H7单克隆抗体杂交瘤细胞株的建立[J].江苏农业学报，2009，25(2):291-295.
[10]夏兴霞，王永山，孟祥升，等.大肠杆菌O157:H7单克隆抗体胶体金免疫层析检测试纸条的研制 [J].中国动物传染病学报，2010,18(4):47-53.
[11]Wadolkowski EA,Burris JA,O’Brien AD.Mouse model for colonization and disease caused by enterohemorrhagic Escherichia coli O157:H7 [J].Infect Immun,1990,58(8):2438 -2445.
[12]Fujii J,Kita T,Yoshida S,et al.Direct evidence of neuron impairment by oral infection with verotoxin-producing Escherichia coli O157:H7 in mitomycin-treated mice [J].Infect Immun,1994,62(8):3447 -3453.

相似文献/References:

[1]吕 恒,张锦海,顾海涛,等.肠出血性大肠杆菌O157:H7的快速可视化检测[J].医学研究与战创伤救治(原医学研究生学报),2013,15(04):321.[doi:10.3969/j.issn.1672-271X.2013.04.001]
　LV Heng,ZHANG Jin-hai,GU Hai-tao,et al.A quicker and visual method for detecting Enterohemorrhagic Escherichia coli O157:H7[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(01):321.[doi:10.3969/j.issn.1672-271X.2013.04.001]

备注/Memo

备注/Memo:
“十一五”国家科技支撑计划(2007BAD40B01)
更新日期/Last Update: 2012-01-20