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[1]丁博文,嵇 武,白小武,等.大黄素对肠上皮细胞损伤的保护作用及机制研究[J].医学研究与战创伤救治(原医学研究生学报),2012,14(03):199-202.
　DING Bo-wen,JI Wu,BAI Xiao-wu,et al.Protective effects of emodin on intestinal epithelial cells injury[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(03):199-202.
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大黄素对肠上皮细胞损伤的保护作用及机制研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年03期
页码:
199-202
栏目:
出版日期:
2012-05-22

文章信息/Info

Title:
Protective effects of emodin on intestinal epithelial cells injury
文章编号:
1672-271X(2012)03-0199-04
作者:
丁博文; 嵇 武; 白小武; 车金辉; 张 强; 李秋荣
210002 江苏南京，南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所
Author(s):
DING Bo-wen; JI Wu; BAI Xiao-wu; Che Jin-hui; ZHANG Qiang; LI Qiu-rong.
Research Institute of General Surgery,Clinical School of Medical College of Nanjing University/Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China
关键词:
大黄素; 紧密连接; Caco-2细胞; 缺氧/复氧
Keywords:
emodin;  tight junction;  Caco-2 cells;  hypoxia/reoxygenation
分类号:
R329.445
DOI:
-
文献标志码:
A
摘要:
目的 观察缺氧/复氧(hypoxia/reoxygenation,H/R)损伤条件下大黄素(Emodin)对肠上皮细胞表达紧密连接蛋白Occludin和ZO-1的影响，以研究大黄素对肠黏膜屏障的保护作用机制。方法 体外培养肠上皮细胞株Caco-2建立H/R模型，将细胞随机分为正常细胞(N)组、缺氧复氧(H/R)组、缺氧复氧+Hank平衡盐溶液(H/R+HBSS)组、缺氧复氧+大黄素(H/R+EN)组。检测跨上皮细胞电阻(transepithelial electrical resistance，TEER)值；使用透射电子显微镜观察紧密连接的变化；用Western blot法测定Occludin和ZO-1蛋白的表达水平；半定量RT-PCR法测定Occludin和ZO-1的mRNA表达水平。结果 与H/R组、H/R+HBSS组相比，H/R+EN组的紧密连接破坏明显减少； H/R+EN组的TEER值、Occludin和 ZO-1的蛋白及mRNA表达水平明显高于H/R组、H/R+HBSS组(P＜0.05)。结论 大黄素可通过减少Occludin、ZO-1的破坏并增加其表达来保护肠黏膜屏障。
Abstract:
Objective Through observing the effects of emodin on the tight junction (TJ)protein Occludin and ZO-1 of intestinal epithelial cells damaged by hypoxia/reoxygenation (H/R),to study the mechanism of the protective effects of emodin on the intestinal mucosa barrier.Methods Bowel epithelial cell lines Caco-2 cells were cultured in vitro to establish H/R model.The cells were averagely randomized into four groups :N (normal cells)group,H/R (hypoxia/reoxygenation)group,H/R+HBSS (hypoxia/reoxygenation and Hanks balanced solution)group,H/R+EN (hypoxia/ reoxygenation and Emodin)group.The transepithelial electrical resistance (TEER)value was measured; transmission electron microscope was used to observe the changes of TJ; Western blot analysis was employed to detect the expression level of Occludin and ZO-1 protein; Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR)was used to detect the mRNA expression level of Occludin and ZO-1.Results Comparing with the H/R group and H/R+HBSS group,the damage extent of TJ was significantly reduced in the H/R+EN group.The TEER value,the protein and mRNA expression level of Occludin and ZO-1 in the H/R+EN group were obviously higher than that of the H/R group and H/R+HBSS group (P＜0.05).Conclusion Emodin can decrease the damage of Occludin and ZO-1,and increasing their expression to protect the intestinal mucosa barrier.

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备注/Memo

备注/Memo:
南京军区医药卫生科研基金课题(09Z027)
更新日期/Last Update: 2012-05-20