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[1]吴 升,沈亚伟,郝 建,等.猪胰蛋白酶致大鼠COPD氧化应激与动脉血气间的相关性研究[J].医学研究与战创伤救治(原医学研究生学报),2012,14(03):219.
　WU Sheng,SHEN Ya-wei,HAO Jian,et al.The research of relationship between oxidatie stress and the changes of arterial blood gas in the rat with COPD induced by pig trypsin[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(03):219.
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猪胰蛋白酶致大鼠COPD氧化应激与动脉血气间的相关性研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年03期
页码:
219
栏目:
出版日期:
2012-05-22

文章信息/Info

Title:
The research of relationship between oxidatie stress and the changes of arterial blood gas in the rat with COPD induced by pig trypsin
文章编号:
1672-271X(2012)03-0219-04
作者:
吴 升; 沈亚伟; 郝 建; 常月进; 刘 娟
310007 浙江杭州，安徽医科大学解放军杭州临床学院(南京军区杭州疗养院)
Author(s):
WU Sheng; SHEN Ya-wei; HAO Jian; CHANG Yue-jing; LIU Juan.
Hangzhou Sanatorium of Nanjing Military Command，Military Clinical College of Hangzhou,Anhui University of Medicine,Hangzhou，Zhejiang 310007,China
关键词:
猪蛋白酶; 大鼠; 慢性阻塞性肺疾病; 氧化应激; 动脉血气分析
Keywords:
porcine pancreatic elastase;  rat;  COPD;  oxidative stress;  arterial blood gas analysis
分类号:
R563.9
DOI:
-
文献标志码:
A
摘要:
目的 探讨猪胰蛋白酶(PPE)致大鼠慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)氧化应激与动脉血气变化的关系。方法 Wistar大鼠30只随机分为对照组(A组)、模型组(B组)和棉籽油组(C组)，每组10只。B、C组大鼠气管内一次性注入PPE构建肺气肿模型，C组大鼠腹腔内注射棉籽油(1 ml/kg)。饲养30 d后处死解剖，测量大鼠肺体积，肺泡形态学，肺组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和测定动脉血气分析，并用统计学方法探讨氧化应激与动脉血气间的相互关系。结果 B、C组大鼠肺体积、肺泡形态学、氧化应激水平均明显大于A组(P<0.05)；B、C组肺组织SOD活力和MDA含量较A组显著升高(P均<0.05)。A组动脉血氧分压(PaO2)、动脉血氧饱和度(SaO2)、动脉血二氧化碳分压(PaCO2)基本正常；B、C组PaO2、SaO2明显降低，而PaCO2明显升高。肺组织SOD 与PaO2呈正相关，肺组织MDA与PaO2呈负相关；肺组织SOD、MDA均与PaCO2 不相关。结论 用猪胰蛋白酶制作大鼠COPD模型成功，大鼠COPD氧化应激与动脉血气之间有着密切的相关性，值得在动物COPD实验造模中推广使用。
Abstract:
Objective To explore the research of relationship between oxidatie stress and the changes of arterial blood gas in the rat with COPD induced by pig trypsin (PPE).Methods Thirty Wistar rats were randomly divided into control group (group A),model group (group B)and cottonseed oil group (group C),each group of each 10 rats.Established model in rats which rats trachea injected for PPE,raising 30 days put to death,and the measurement of rats lung volume,the alveolar morphology,content of SOD and MDA of lung tissue and determination of arterial blood gas analysis,and statistical methods to explore of relation oxidatie stress with arterial blood gas.Results The lung volume，the alveolar morphology,oxidative stress levels in group B and C were significantly greater than A group (P<0.05); Content of SOD and MDA in B and C group of lung tissue were increased significantly than group A (P<0.05).PaO2,SaO2 ,PaCO2 in Group A was basic normal; PaO2 and SaO2 in B,C group were significantly reduced,and PaCO2 was increased significantly.SOD of lung tissue was positively associated with PaO2,MDA of lung tissue were negatively correlated PaO2; MDA and SOD of lung tissue were not related with PaCO2.Conclusion With pig trypsin make rats model of COPD,the rats with COPD has close correlation between oxidation stress and arterial blood gas,the animal experiment mode of COPD is worthy of use of promotion.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-05-20