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[1]夏维木,刘定益,周文龙,等.新辅助治疗后RT-PCR对前列腺癌盆腔淋巴结微转移的诊断价值[J].医学研究与战创伤救治(原医学研究生学报),2012,14(05):399-401.
　XIA Wei-mu,LIU Ding-yi,ZHOU Wen-long,et al.The diagnostic value of real-time reverse-transcriptase polymerase chain reaction in detecting micrometastases of pelvic lymph nodes after neoadjuvant hormonal therapy[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(05):399-401.
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新辅助治疗后RT-PCR对前列腺癌盆腔淋巴结微转移的诊断价值()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年05期
页码:
399-401
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
The diagnostic value of real-time reverse-transcriptase polymerase chain reaction in detecting micrometastases of pelvic lymph nodes after neoadjuvant hormonal therapy
文章编号:
1672-271X(2012)05-0399-03
作者:
夏维木1; 刘定益2; 周文龙3; 王名伟4; 王 健2; 王 颖5; 王树军5; 叶永峰1; 王满琴1; 许 胜1; 夏 宇1
1.335000 江西鹰潭,解放军184医院泌尿外科；2.200125 上海,上海市浦东新区浦南医院泌尿外科；3.200025 上海,上海交通大学医学院附属瑞金医院泌尿外科；4.200020 上海,上海交通大学医学院附属瑞金医院卢湾分院泌尿外科；5.200025 上海,上海交通大学医学院
Author(s):
XIA Wei-mu1; LIU Ding-yi2; ZHOU Wen-long3; WANG Ming-wei4; WANG Jian2; WANG Ying5; WANG Shu-jun5; YE Yong-feng1; WANG Man-qin1; XU Sheng1; Xia Yu1
1.Department of Urology,184 Hospital of PLA,Yingtan,Jiangxi 335000,China;2.Department of Urology,Punan Hospital,Shanghai 200125,China;3.Department of Urology,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China;4.Department of Urology,Luwan Branch of Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200020,China;5.Shanghai Jiaotong University School of Medicine,Shanghai 200025,China
关键词:
逆转录酶链反应; 前列腺肿瘤;  前列腺癌根治术; 微转移; 新辅助治疗
Keywords:
quantitative real-time PCR;  prostate cancer;  radical prostatectomy;  micrometastasis;  neoadjuvant hormonal therapy
分类号:
R737.25
DOI:
-
文献标志码:
A
摘要:
目的 明确新辅助治疗后以逆转录酶-聚合酶链反应(RT-PCR)法检测前列腺癌盆腔淋巴结微转移的意义。方法 本组41例临床局限性前列腺癌病例，术前行淋巴管造影显示盆腔淋巴结，对可疑淋巴结在X线定位下穿刺抽吸淋巴液，用RT-PCR法检测淋巴液中前列腺特异性抗原(prostate-specific antigen，PSA)mRNA和前列腺特异性膜抗原(prostate-specific membrane antigen，PSMA)mRNA的表达；术后对淋巴结组织切片进行免疫组化检查,将PSA mRNA或PSMA mRNA阳性作为存在微转移。结果 对术前盆腔淋巴结穿刺抽吸淋巴液测PSA mRNA和PSMA mRNA，证实有21例淋巴结存在微转移，术后对清扫淋巴结予免疫组化检查有5例存在淋巴结转移，组织学检查阳性组与RT-PCR检测证实微转移组PSA mRNA和PSMA mRNA的表达存在明显差异。结论 新辅助治疗后，采用RT-PCR法检测淋巴液中PSA mRNA和PSMA mRNA的表达有利于探测到淋巴结微转移。
Abstract:
Objective To clarify the significance of real-time reverse-transcriptase polymerase chain reaction (RT-PCR) in detecting micrometastases of pelvic lymph nodes after neoadjuvant hormonal therapy.Methods The study included 41 patients with clinically localized prostate cancer who received neoadjuvant hormonal therapy (NHT).If positive lymph nodes are seen or suspected,a thin-walled 22 gauge needle is directed transabdomenally under fluoroscopic control into the area and an aspirate is obtained after bipedal lymphangiography.The expression of prostate-specific antigen (PSA) and prostate-specific membrane antigen (PAMA) in aspirate were assessed by a fully quantitative real-time reverse-transcriptase polymerase chain reaction.We regarded specimens in which either PSA or PSMAmRNA were positive as showing the “presence of micrometastasis”.Lymph node specimens were also stained immunohistochemically with an antibody PSA after RP.Results Lymph node metastases from 5 cases were detected bt pathological examination,and real-time RT-PCR further identified lymph node micrometastases from 21 cases with no pathological evidence of nodal involvement.The expression level of PSA mRNA and PSMA mRNA were statistically significant in patients with histological confirmed lymph node metastases and micrometastases detected by real-time RT-PCR despite the lack of histological evidence.Conclusion Applying quantitative real-time RT-PCR targeting PSA and PSMA genes can detect micrometastatic tumour foci in pelvic lymph nodes by fine needle aspiration biopsy of lymph nodes.

参考文献/References:

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备注/Memo

备注/Memo:
南京军区医学科技创新经费资助项目(07MO58)
更新日期/Last Update: 2012-09-20