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[1]王 波,张存海,徐梦洁,等.大容量人血小板冻干保存的初步研究[J].医学研究与战创伤救治(原医学研究生学报),2012,14(05):413-415.
　WANG Bo,ZHANG Cun-hai,XU Meng-jie,et al.Preliminary study on the preservation of large volume of human platelets by freeze-drying[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(05):413-415.
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大容量人血小板冻干保存的初步研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年05期
页码:
413-415
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
Preliminary study on the preservation of large volume of human platelets by freeze-drying
文章编号:
1672-271X(2012)05-0413-03
作者:
王 波1; 张存海1; 徐梦洁2; 许先国3; 陈光明2; 张绍志2
1.310013 浙江杭州,解放军117医院重症医学科;2.310027 浙江杭州,浙江大学制冷与低温研究所；3.310006 浙江杭州,浙江省血液中心输血研究所
Author(s):
WANG Bo1; ZHANG Cun-hai1; XU Meng-jie2; XU Xian-guo3; CHEN Guang-ming2; ZHANG Shao-zhi2
1.Intensive Care Unit ,117 Hospital of PLA,Hangzhou,Zhejiang 310013,China;2.Institute of Refrigeration and Cryogenics,Zhejiang University,Hangzhou,Zhejiang 310027,China;3.Institute of Blood Transfusion,Blood Center of Zhejiang Province,Hangzhou,Zhejiang 310006,China
关键词:
血小板; 冷冻干燥; 大容量; 聚集; 流式细胞术
Keywords:
platelet;  freeze-drying;  large volume;  aggregation;  flow cytometry
分类号:
R457
DOI:
-
文献标志码:
A
摘要:
目的 为使血小板冷冻干燥保存技术用于军队临床战创伤救治，在小容量冻干保存的基础上研究大容量人血小板的冷冻干燥技术。方法 采用特制血盒作为冻干容器，以20%(w/v)海藻糖和1%(w/v)牛血清白蛋白作为冻干保护剂。冻干样品复水后测量血小板的数值恢复率和体积分布参数，应用流式细胞仪检测血小板的凋亡率和活化率，采用二磷酸腺苷和肾上腺素检测血小板的聚集功能。结果 大容量血小板冻干复水后的数值恢复率为(66.3±2.1)%，平均血小板体积较新鲜血小板有明显增加，冻干血小板的细胞凋亡率达20%左右；冻干和复水过程还造成小部分血小板被激活，其最大聚集率为新鲜血小板的50%左右。结论 大容量血小板冻干复水后的恢复率较低，形态结构、活性和功能指标均低于新鲜血小板，但基本可以满足血小板临床止血功能标准。
Abstract:
Objective Towards a clinical application of the technology of freeze-drying preservation of human platelets in certain military situations,a primary study on the freeze-drying of large volume of human platelets was conducted.Methods A specially designed blood vessel was used as the lyophilization container.The human platelets were rehydrated directly after lyophiliztion.The cell recovery rate,mean platelet volume and platelet distribution width were analyzed by a blood-cell counter.The apoptosis rate and activation rate were recorded by a flow cytometry.The aggregation function was tested with adenosine diphosphate and epinephrine.Results The recovery rate of rehydrated platelets reached (66.3±2.1)%.Compared with fresh control the mean platelet volume of rehydrated platelets increased significantly.The apoptosis rate was about 20% according to the analysis of the flow cytometry,and a small part of platelets were activated during the freeze-drying and rehydration process.The maximal aggregation rate was approaching approximately 50% of that of fresh control.Conclusion Freeze-drying preservation of large volume of human platelets resulted in a comparatively low cell recovery rate compared with that of small volumes,and the morphology,aggregation function was also affected,but meet the clinical requirement of coagulation on the whole.

参考文献/References:

[1]Kanias T,Acker JP.Mammalian Cell Desiccation:Facing the Challenges[J].Cell Preserv Technol,2006,4(4):253-277.
[2]Holovati JL,Hannon JL,Gyongyossy-Issa MIC,et al.Blood preservation workshop:new and emerging trends in research and clinical practice[J].Transfusion Medicine Review,2009,23(1):25-41.
[3]Zhang SZ,Fan JL,Xu XG,et al.An experimental study of the use of ultrasound to facilitate the loading of trehalose into platelets[J].Cryobiology,2009,59(2):135-140.
[4]Han Y,Quan GB,Liu XZ,et al.Improved preservation of human red blood cells by lyophilization[J].Cryobiology,2005,51(2):152-164.
[5]陈麟凤,刘景汉,汪德清,等.人红细胞冻干后复水条件的初步研究[J].中国实验血液学杂志,2009,17(6):1582-1587.
[6]Li J,Hua TC,Gu XL,et al.Morphology study of freeze-drying mononuclear cells of human cord blood[J].Cryo Letters,2005,26(3):193-200.
[7]Wolkers WF,Walker NJ,Tamari YE,et al.Towards a clinical application of freeze-dried human platelets[J].Cell Preserv Technol,2003,1(3):175-188.
[8]周新丽.人血小板冷冻干燥保存的实验研究[D].浙江大学博士学位论文,2006.
[9]Zhou XL,Zhu H,Zhang SZ,et al.Freeze-drying of human platelets:influence of intracellular trehalose and extracellular protectants[J].Cryo Letters,2006,27(1):43-50.
[10]Fan JL,Xu XG,Zhang SZ,et al.Experimental study on rehydration conditions of freeze-dried platelets[J].Journal of Zhejiang University-SCIENCE A,2009,10(5):697-703.
[11]Hoshi R,Murata S,Matsuo R,et al.Freeze-dried platelets promote hepatocyte proliferation in mice[J].Cryobiology,2007,55(3):255-260.
[12]冯智慧,胡 彬,刘 棋.血小板保存的研究进展[J].中国输血杂志,2009,22(10):854-856.
[13]焦留宏，吴德平，赵 兵.某部4所医院科研情况调查分析[J].东南国防医药,2011,13(5):430-432.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-09-20