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[1]陈 勇,胡毓安,袁大莉,等.乙型肝炎病毒表面大蛋白的检测及其临床应用[J].医学研究与战创伤救治(原医学研究生学报),2012,14(06):525-527.
　CHEN Yong,HU Yu-an,YUAN Da-li,et al.Detection of HBV large surface protein and its clinical significance in patients with hepatitis B[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(06):525-527.
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乙型肝炎病毒表面大蛋白的检测及其临床应用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年06期
页码:
525-527
栏目:
出版日期:
2012-11-20

文章信息/Info

Title:
Detection of HBV large surface protein and its clinical significance in patients with hepatitis B
文章编号:
1672-271X(2012)06-0525-03
作者:
陈 勇; 胡毓安; 袁大莉; 袁静文
210002 江苏南京，南京大学医学院(南京军区南京总医院)临床中心实验科
Author(s):
CHEN Yong;  HU Yu-an;  YUAN Da-li; YUAN Jing-wen
Nanjing University School of Medicine,Istitute of Medical Laboratory Sciences of PLA,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China
关键词:
乙型肝炎病毒表面大蛋白; 乙型肝炎病毒脱氧核糖核酸; 酶联免疫吸附测定; 聚合酶链反应
Keywords:
LHBs;  HBV DNA;  ELISA;  PCR
分类号:
R512.62
DOI:
-
文献标志码:
A
摘要:
目的 检测乙型肝炎病毒表面大蛋白(hepatitis B virus large surface protein,LHBs),并与乙型肝炎病毒脱氧核糖核酸(HBV DNA)相比较，探讨LHBs的临床应用价值。方法 分别采用ELISA和PCR法检测乙型肝炎257例血清中LHBs，乙型肝炎病毒标志物(HBV-M)和HBV DNA，并进行相关性分析。结果 LHBs与HBV DNA的检出率差异无统计学意义(P>0.05)；LHBs的阳性率(83.12%)高于乙肝e抗原(HBeAg)的阳性率(54.63%)，差异有统计学意义(P<0.05)。结论 血清中LHBs水平能反映HBV感染者体内的病毒复制情况，将其作为HBV复制指标，灵敏度高于HBeAg,可作为反映HBV感染者体内的病毒复制情况的新的血清学指标，尤其是针对HBeAg阴性患者。
Abstract:
Objective To explore the clinical significance of Hepatitis B virus large surface protein (LHBs) by detecting LHBs.Methods The LHBs were detected by using enzyme linked immunosorbent assay (ELISA).Surum HBV DNA was detected by using real-time polymerase chain reaction (RT-PCR) and ELISA was used to detect the HBV markers in 257 serum samples from hepatitis B patients.Results No significant difference was found between the positive rate of LHBs and that of HBV DNA(P＞0.05).The positive rate of LHBs was 83.12%,which was higher than that of HBeAg which was 54.63%.There was significant diference (P＜0.05).Conclusion The level of serum LHBs can be used to estimate the state of HBV replication and the sensitivity was superior to HBeAg.It may be used as a new serological maker to detect HBV replication,especially in HBeAg negative patients.

参考文献/References:

[1]成 均，孙长贵，戴玉柱，等.驻浙部队HBV感染情况及低浓度HBsAg流行病学调查[J].东南国防医药，2009，17(4):298-301.
[2]时连华，郑纪山.乙型肝炎病毒血清亚型的研究进展[J].东南国防医药，2003，5(5):398-399.
[3]Bang G，kim KH,Gnarnieri M,et al.Ehect of mutating the two cysteines reguired for HBe autigenicity on hepatitis B virus DNA replication and virion secretion[J].Virology,2005，332(1):216-224.
[4]Chua PK,Wang RY,Lin MH,et al.Reduced secretion of virions and hepatitis B virus (HBV) surface antigen of a naturally occurring HBV variant correlates with the accumulation of the small S envelope protein in the endoplasmic reticulum and golgi apparatus[J].J Virol,2005,7(9):13483-13496.
[5]Hamassak K,Nakata K,Nagagama Y,et al.Chang in prevalence of HBeAg negative mutant hepatitis B virus during the course chronic hepatitis B[J].Hepatology,1994,20(5):8-14.
[6]Lambert C,Mann S,Prange R.Assessment of determinants affecting the dual topology of hepadnaviral large envelope proteins[J].J General Virol,2004,8(5):1221-1225.
[7]Foo NC，Ahn B,Ma X，et al．Cellular vacuolization and apoptosis induced by hepatitis B virus large surface protein[J].Hepatology,2002,6(3):1400-1407．
[8]Bruss V.Envelopment of the hepatitis B virus nucleocapsid[J].Virus Res,2004,106(2):199-209.
[9]Bruss M.Miska S,Chassot S.Enhancement of hepatitis B virus in 2 fection by non-infections subviral particles[J].J Virol,1998,72(2):1462-1468.
[10]毛远丽，李伯安，马洪滨，等．乙肝患者外膜蛋白血清学检测及对于判定HBV DNA复制的意义[J]．中华实验和临床病毒学杂志，2006，20(3):276-278．
[11]乐爱平，鞠北华，王 文，等．乙肝病毒大蛋白在乙型肝炎诊治中的临床意义[J]．世界华人消化杂志，2006，14(16):1578-1581．
[12]魏红山，黄玉波．宋淑静，等．e抗原阴性慢性乙型肝炎患者血清表面抗原大蛋白水平与乙型肝炎病毒DNA之问的关系[J]．中华肝脏病杂志，2006，14(7):543-546．
[13]乐爱平，胡国信．乙肝病毒大蛋白在血清HBeAg阴性与低水平HBV DNA乙肝患者中的检测意义[J]．现代免疫学杂志，2008，28(3):244-247．
[14]孙 颖，辛绍波，雷 厉，等.乙肝病毒外膜大蛋白检测对于判定HBV DNA复制的意义[J].世界华人消化杂志，2006，14(3):354-357.
[15]吴正林，刘 玢，肖桂初，等.乙型肝炎病毒表面大蛋白与HBV DNA检测的对比研究[J].中华实验诊断学，2007，11(4):479-481.

相似文献/References:

[1]陈 勇,胡毓安.乙型肝炎病毒表面大蛋白的研究进展[J].医学研究与战创伤救治(原医学研究生学报),2013,15(04):381.[doi:10.3969/j.issn.1672-271X.2013.04.020]

备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-11-20