[1]李晶晶,李国利.VEGF、p53在精原细胞瘤中的表达及相关性分析[J].医学研究与战创伤救治(原医学研究生学报),2013,15(03):262-264.[doi:10.3969/j.issn.1672-271X.2013.03.018]
LI Jing-jing,LI Guo-li.Relationship between expression of VEGF and p53 genes and clinicopathologicalparameters in seminoma of testis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(03):262-264.[doi:10.3969/j.issn.1672-271X.2013.03.018]
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VEGF、p53在精原细胞瘤中的表达及相关性分析(
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LI Jing-jing,LI Guo-li.Relationship between expression of VEGF and p53 genes and clinicopathologicalparameters in seminoma of testis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(03):262-264.[doi:10.3969/j.issn.1672-271X.2013.03.018]
)《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]
- 卷:
- 第15卷
- 期数:
- 2013年03期
- 页码:
- 262-264
- 栏目:
- 出版日期:
- 2013-03-30
文章信息/Info
- Title:
- Relationship between expression of VEGF and p53 genes and clinicopathologicalparameters in seminoma of testis
- 分类号:
- R730.2
- 文献标志码:
- A
- 摘要:
- 目的 通过对人血管内皮生长因子(vascular endothelial cell growth factor,VEGF)、p53基因的研究,从分子水平上研究精原细胞瘤恶变过程中癌基因、抑癌基因表达的改变,为早期明确诊断和治疗精原细胞瘤提供分子生物学上的依据。方法 应用免疫组化辣根过氧化物酶标记的链霉卵白素(streptavidin-peroxidase,S-P)法,检测VEGF、p53基因在38例精原细胞瘤中的表达。应用PCR-单链构象多态性(PCR-SSCP)法,检测p53在精原细胞瘤中的突变及缺失情况。结果 VEGF在精原细胞瘤临床分期Ⅰ期的阳性表达率为33.33%,Ⅱ期和Ⅲ期的阳性表达率分别为90.91%和100%,p53在精原细胞瘤临床分期Ⅰ期的阳性表达率为93.33%,Ⅱ期与Ⅲ期的表达率分别为54.55%和50%,VEGF和p53基因在精原细胞瘤中的表达无相关性。p53在精原细胞瘤中存在突变,突变率为45.45%。结论 VEGF在精原细胞瘤的发生中发挥着重要作用; p53在精原细胞瘤的发生和发展过程中,存在着基因表达的异常。p53在调控精原细胞瘤发生过程中,主要在临床发生的早期起重要作用; 精原细胞瘤中,p53与VEGF不存在相关性。
- Abstract:
- Objective To investigate the tumor and tumor suppressive genes in the carcinogenesis of seminoma of testis byexploring the expression of VEGF and p53,and to provide the molecular biologic basis for the early identified diagnostic and treatment of seminoma.Methods 38 paraffinembeded specimens of seminoma were collected.IHC was used to detect the expression of protein of VEGF and p53.PCR-SSCP was used to detect the mutation and deletion of p53.Results The positive expression rates of VEGF in seminoma clinical stageⅠ,Ⅱ,Ⅲ were 33.33%,90.91%,100%,respectively.The positive expression rate of p53 in seminoma clinical stageⅠ,Ⅱ,Ⅲ were 93.33%,54.55%,50%.There was no correlation between expression of VEGF and p53 in seminoma.The mutation rate in seminoma was 45.45%.Conclusion The VEGF and p53 play important roles in the development and progression of seminoma.There is an altered expression of p53 in the progression of seminoma and the p53 gene plays important roles in the early stage.No relationship between p53 and VEGF was found.
参考文献/References:
[1] 刘仁伟,吴志清,冯丰坔,等.睾丸精原细胞瘤的MRI表现[J].中国医学影像技术,2012,28(5):982-985. [2] 牛晓东,王海平,黄 勇.睾丸精原细胞瘤伴血β-hCG增高一例报告并文献复习[J].解放军医药杂志,2012,24(1):69-71. [3] Beahrs OH,Henson DE,Hutter RVP,et al.American joint committee on cancer manual for staging of cancer[M].Philadelphia,JB Lippincot Co,1992: 83. [4] Ferrara N,Henzel WJ.Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells [J].Biochem Biophys Res Commun,1989,161(2):851-858. [5] 任庆琎.结肠癌组织中Survivin、基质金属蛋白酶-9和血管内皮生长因子的表达及临床意义[J].中国实用医刊,2012,39(16): 8-9. [6] 赵海滨,张伟杰,周志华.MMP-7、MMP-9、VEGF-C、VEGF-D在胃癌中的表达及其临床意义[J]东南国防医药,2010,12(3):197-199. [7] 马 波,赵吉生,丁大勇.p53 、VEGF 的表达与胃癌生物学行为相关性的研究[J].中国实验诊断学,2009,9(6):920-922. [8] Restucci B,Maiolino P,Paciello O,et al.Evaluation of angiogenesis in canine seminomas by quantitative immunohistochemistry[J].Comp Pathol,2010,128(4):252-259. [9] 周晓英,舒琦瑾.肺癌相关指标与中医证型的相关性[J].中华中医药学刊,2012,30(4):885-887. [10] 苏 蔚,房 成,赵卫星.食管癌中p53、VEGF-C 的表达与MVD 相关性研究[J].肿瘤基础与临床,2009,19(4):279-281. [11] Lewis DJ,Sesterhenn IA,Moul JW,et al.Immunohistochemical expression of p53 tumor suppressor gene protein in adult germ cell testis tumors:clinical correlation in stage I disease [J].J Uro,2009,15(2):418-423. [12] Eid H,VandeLooij M,Bak M,et al.Is p53 expression,detected by immunohistochemistry,an important parameter of response to treatment in testis cancer?[J]. Anticancer Res,2007,17(4A):2663-2669.
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