[1]张立波,夏欣一,马贵明,等.以转录介导扩增技术大规模筛查无偿献血者的应用研究[J].医学研究与战创伤救治(原医学研究生学报),2014,16(01):6-9.
ZHANG Li-bo,XIA Xin-yi,MA Gui-ming,et al.Application of transcription mediated amplification (TMA) technique in large scale voluntary blood donor screening[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(01):6-9.
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以转录介导扩增技术大规模筛查无偿献血者的应用研究(
)
ZHANG Li-bo,XIA Xin-yi,MA Gui-ming,et al.Application of transcription mediated amplification (TMA) technique in large scale voluntary blood donor screening[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(01):6-9.
)《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]
- 卷:
- 第16卷
- 期数:
- 2014年01期
- 页码:
- 6-9
- 栏目:
- 出版日期:
- 2014-02-20
文章信息/Info
- Title:
- Application of transcription mediated amplification (TMA) technique in large scale voluntary blood donor screening
- Keywords:
- nucleic acid test (NAT); transcription mediated amplification(TMA); window period; blood safety
- 分类号:
- R446.5
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 目的 研究和探讨以转录介导扩增(transcription mediated amplification,TMA)技术对无偿献血者进行大规模病毒核酸检测，以增强临床使用血液的安全性。方法 以TMA技术对无偿献血者血液进行HBV DNA、HCV RNA、HIV1 RNA 3项联合病毒核酸检测，同时对上述样本以 ELISA技术 检测HIV、HBV和HCV抗原或抗体。结果 54 744份献血者血液中以TMA技术共检出病毒核酸阳性样本208例，阳性检出率为0.38%。ELISA检出阴性而TMA检出阳性的样本共75份，阳性率为0.14%，其中病毒核酸阳性共46份，即HBV DNA阳性45份与HIV1 RNA阳性1份，HCV RNA阳性未检出视为不确定结果29份。ELISA阴性而病毒核酸阳性共46份，ELISA检测漏检率为0.08%。结论 以TMA技术对无偿献血者血液进行大规模病毒核酸检测能有效缩短无偿献血人群中HIV、HBV和HCV检测的“窗口期”，显著降低输血可能存在的风险。
- Abstract:
- Objective To research and investigate the effect of applying the viral nucleic acid test (NAT) on the safety of blood transfusion in large scale.Methods Voluntary donors’ blood samples were tested with TMA technique by detecting HBV DNA,HCV RNA,HIV1 RNA nucleic acid.The samples were test with ELISA at the same,by detecting HIV/HBV/HCV antigen or antibody.Results 208 viral nucleic acid positive samples were detected out of 54 744 donors’ blood samples and the positive rate was 0.38%.ELISA negative,NAT positive samples were further tested with discrimination assays.45 samples were confirmed as HBV DNA positive,none as HCV RNA positive and 1 as HIV1 RNA positive.Conclusion TMA can efficiently shorten the window period of HIV,HBV and HCV infection in blood screening in large scale,therefore significantly decrease the potential risks of blood transfusion.
参考文献/References:
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