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[1]黄 梅,王 颖,王卫萍,等.两种吸附抗生素血培养瓶对血流感染检测能力的比较[J].医学研究与战创伤救治(原医学研究生学报),2014,16(02):117-120.[doi:10.3969/j.issn.1672-271X.2014.02.002]
　HUANG Mei,WANG Ying,WANG Wei-ping,et al.Compare the detection ability of 2 kinds blood cultured bottle which can absorb the antibiotics[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(02):117-120.[doi:10.3969/j.issn.1672-271X.2014.02.002]
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两种吸附抗生素血培养瓶对血流感染检测能力的比较()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年02期
页码:
117-120
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Compare the detection ability of 2 kinds blood cultured bottle which can absorb the antibiotics
作者:
黄 梅1; 王 颖1; 王卫萍1; 史利宁1; 李东兵2; 邵海枫1; 李芳秋1
1.210002 江苏南京，南京大学医学院附属金陵医院解放军临床检验医学研究所;2.210000 江苏南京，解放军94608部队
Author(s):
HUANG Mei1; WANG Ying1; WANG Wei-ping1; SHI Li-ning1; LI Dong-bing2; SHAO Hai-feng1; LI Fang-qiu1.
1.Institute of Clinical Laboratory,Jinling Hospital Affiliated of Nanjing University,Nanjing,Jiangsu 210002,China;2.94608 Army of PLA，Nanjing，Jiangsu 210000,China
关键词:
血培养; BACTEC PLUS树脂需氧培养瓶; BacT/Alert FA活性炭需氧培养瓶; 血流感染
Keywords:
blood culture; BACTEC PLUS resin oxygen bottle; BacT/Alert FA active carbon oxygen bottle (Bio-FA bottles); bloodstream infection
分类号:
R446.51
DOI:
10.3969/j.issn.1672-271X.2014.02.002
文献标志码:
A
摘要:
目的 比较具有吸附抗生素能力的2种血培养瓶，即BACTEC PLUS树脂需氧瓶(简称BD-P瓶)与BacT/Alert FA活性炭需氧瓶(简称Bio-FA 瓶)对血流感染的检测能力。 方法 每位患者从两个不同部位采集血标本分别注入BD-P瓶和Bio-FA瓶，放入相应培养仪进行培养，记录报阳时间。结合临床用药资料对这2种具有不同吸附剂的血培养瓶的阳性检出率及报阳时间进行比较。 结果 在6670瓶血培养中阳性报警共1017瓶，确认污染196瓶(2.94%)，有临床意义的阳性821瓶(12.31 %)。共检出病原菌493株，其中双瓶阳性334例(67.75%)；BD-P瓶单瓶阳性102例(20.69%)，Bio-FA瓶单瓶阳性57例(11.56%)。BD-P瓶对病原菌的检出率优于Bio-FA瓶(P<0.01)，对于肠杆菌科和葡萄球菌的检出率BD-P瓶明显高于Bio-FA瓶(P<0.01)。BD-P瓶细菌报阳时间早于Bio-FA瓶(P<0.01)，特别是对肠杆菌科和非发酵菌的阳性报警时间明显早于Bio-FA瓶。由正在使用药物治疗的患者中检出344例阳性，其BD-P瓶的阳性检出率和报阳时间均高于和快于Bio-FA瓶(P<0.05)；按不同的治疗药物进行分组，对头孢哌酮/舒巴坦BD-P瓶的吸附能力更强(P<0.05)。结论 应用BD-P瓶检测血流感染较Bio-FA瓶检出率更高，检测速度更快。而联合使用Bio-FA瓶和BD-P瓶，比单用一种培养瓶更具优势。
Abstract:
Objective To compare the detectability to the bloodstream infection by 2 kinds bottles of BACTEC PLUS resin oxygen bottle (BD-P bottles) and BacT/Alert FA active carbon oxygen bottle (Bio-FA bottles).Methods All blood samples of patients from two different parts were injected into BD-P bottles and Bio-FA bottles and cultured in the blood culture system,record positive time.The positive rate and positive time of 2 different adsorbent blood culture bottles were compared combining with clinical data.Results There are 1017 positive bottles between the 6670 bottles,196 bottles were confirmed pollution,accounted for 2.94%;the clinical significance of the positive 821 bottles,accounted for 12.31%.493 pathogenic strains were detected,of which two bottles was positive in 334 patients (67.75%),single BD-P bottle was positive in 102 cases (20.69%);single Bio-FA bottle was positive in 57 cases (11.56%).The detection rate of Bio-FA bottle was higher than that of BD-bottle P (P<0.01),especially for the detection of Enterobacteriaceae and Staphylococcus.BD-P bottle bacteria Bao Yang predates the Bio-FA bottle (P<0.01),especially the positive alarm time for Enterobacteriaceae and non fermentive bacteria significantly earlier in Bio-FA bottles.In 344 positive cases using drug treatment,the positive rate and time of BD-P were higher and faster than that of the Bio-FA bottle (P<0.05);and the adsorption ability of BD-P bottles to cefoperazone/sulbactam was stronger than that of the Bio-FA bottle (P<0.05) according to different therapeutic drugs.Conclusion The detectability of BD-P bottles to the bloodstream infection was higher and faster than that of Bio-FA bottles.Combination of Bio-FA bottle and BD-P bottle was more advantage than using a single bottle.

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备注/Memo

备注/Memo:
国家青年自然科学基金项目(81302536)
更新日期/Last Update: 2014-03-20