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[1]徐 锋,韩从辉,刘有黄,等.miRNA-625*在膀胱尿路上皮癌中的表达[J].医学研究与战创伤救治(原医学研究生学报),2014,16(02):121-123.[doi:10.3969/j.issn.1672-271X.2014.02.003]
　XU Feng,HAN Cong-hui,LIU You-huang,et al.Expression of miRNA-625* in urothelial carcinoma of the bladder[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(02):121-123.[doi:10.3969/j.issn.1672-271X.2014.02.003]
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miRNA-625*在膀胱尿路上皮癌中的表达()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年02期
页码:
121-123
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Expression of miRNA-625* in urothelial carcinoma of the bladder
作者:
徐 锋1; 韩从辉2; 刘有黄1; 高建平1; 张征宇1; 葛京平1; 位志峰1; 程 文1
1.210002 江苏南京，南京军区南京总医院泌尿外科；2.221009 江苏徐州,江苏省徐州市中心医院泌尿外科
Author(s):
XU Feng1; HAN Cong-hui2; LIU You-huang1; GAO Jian-ping1; ZHANG Zheng-yu1; GE Jing-ping1; WEI Zhi-feng1; CHENG Wen1.
1.Department of Urology,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China；2.Department of Urology,the Central Hospital of Xuzhou,Xuzhou,Jiangsu 221009,China
关键词:
miRNA-625*; 膀胱尿路上皮癌; 微小RNA
Keywords:
miRNA-625*; urothelial carcinoma of the bladder; miRNA
分类号:
R737.14
DOI:
10.3969/j.issn.1672-271X.2014.02.003
文献标志码:
A
摘要:
目的 探讨miRNA-625*在膀胱尿路上皮癌中的差异表达。方法 常规提取新鲜膀胱尿路上皮癌与正常膀胱黏膜组织中总RNA；使用分光光度计测定RNA 在260 nm、280 nm和230 nm的吸光值，以计算浓度并评估纯度；用琼脂糖凝胶电泳检测RNA纯度及完整性；以RT-PCR方法检测标本中miRNA-625*的表达情况。结果 RNA质量检测合格后，miRNA-625*经RT-PCR验证与miRNA芯片分析结果一致。与正常膀胱黏膜上皮组织相比，膀胱尿路上皮癌中miRNA-625*表达显著上调(P＜0.05)。结论 miRNA-625*在膀胱尿路上皮癌中表达上调，可作为膀胱尿路上皮癌诊断和预后判断的分子标志物。
Abstract:
Objective To detect the miRNA-625* expression in urothelial carcinoma of the bladder (BUC).Methods BUC and normal bladder mucosa tissues were collected and the total RNA was extracted routinely.Measuring the absorption value of RNA at 260 nm,280 nm,and 230 nm by NanoDrop ND-1000 in order to calculate RNA Quantification and Quality Assurance.RNA Integrity and gDNA contamination Test by Denaturing Agarose Gel Electrophoresis.The miRNA-625* expression was detected by using real-time quantitative polymerase chain reaction.Results RNA Quantification and RNA Integrity were qualified for the follow-up experiment,the miRNA-625* finding was confirmed by real-time RT-PCR and the expression was significantly up-regulated in BUC tissues than those in normal bladder mucosa (P<0.05).Conclusion The expression level of miRNA-625* is significantly up-regulated in BUC,and it may be used for prediction of prognosis and as for targeted treatment of BUC.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金(30772278)
更新日期/Last Update: 2014-03-20