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[1]叶桂娥,刘木彪,黄宜娥,等.VVC与RVVC患者治疗前后阴道菌群的对比研究[J].医学研究与战创伤救治(原医学研究生学报),2014,16(06):600-604.[doi:10.3969/j.issn.1672-271X.2014.06.012]
　YE Gui-e,LIU Mu-biao,HUANG Yi-e,et al.Comparative study of vaginal microflora of VVC and RVVC patients before and after treatment[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(06):600-604.[doi:10.3969/j.issn.1672-271X.2014.06.012]
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VVC与RVVC患者治疗前后阴道菌群的对比研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年06期
页码:
600-604
栏目:
出版日期:
2014-11-20

文章信息/Info

Title:
Comparative study of vaginal microflora of VVC and RVVC patients before and after treatment
作者:
叶桂娥1; 刘木彪1; 黄宜娥2; 周宏伟2
1.510282 广东广州，南方医科大学珠江医院妇产科；2.510515 广东广州，南方医科大学公共卫生与热带医学学院环境卫生学系
Author(s):
YE Gui-e1; LIU Mu-biao1; HUANG Yi-e2; ZHOU Hong-wei2.
1.Department of Obstetrics and Gynecology,Zhujiang Hospital,Southern Medical University,Guangzhou， Guangdong 510282,China；2.Department of Environmmental Health,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou,Guangdong
关键词:
外阴阴道假丝酵母菌病; 复发性外阴阴道假丝酵母菌病; 阴道菌群; 16s rRNA
Keywords:
VVC;  RVVC;  vaginal microflora;  16s rRNA
分类号:
R711.31
DOI:
10.3969/j.issn.1672-271X.2014.06.012
文献标志码:
A
摘要:
目的 从菌群总体角度分析和比较单纯性外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)患者与复发性外阴阴道假丝酵母菌病(recurrent vulvovaginal candidiasis,RVVC)患者临床治疗前后阴道菌群的差异，了解临床治疗与阴道微生态多样性的相关性。方法 采集VVC患者(VVC组，3例)及RVVC患者(RVVC组，3例)女性阴道后穹窿分 泌物，提取细菌总基因组DNA，扩增16s rRNA V4区基因，以得到样品的物种信息用于对比研究。结果 VVC组和RVVC组两组阴道菌群未见显著差异，而以个体为特点区分聚类。用药 后，VVC组菌群的α-多样性未见明显改变，但菌群结构改变显著，体现在乳酸菌比例增加，加德纳菌和变异菌属比例降低。用药后RVVC组菌群的α-多样性和菌群构成均未见显著改 变(P>0.05)。结论 VVC组和RVVC组阴道菌群无显著差异。用药后，VVC组菌群结构改变较RVVC组显著。
Abstract:
Objective To analyze and compare differences of vaginal microflora of patients with VVC and RVVC and explore the correlation of clinical treatment and vaginal microflora diversity after clinical treatments.Methods Twelve vaginal swabs of the vaginal fornix were collected from 3 VVC patients and 3 RVVC patients.Genomic DNA of the samples was extracted for amplifying the 16s rRNA V4 hypervariable region were employed to analyze the information of the species from the samples.Results The vaginal microflora were clustered by individual rather than VVC and RVVC.The VVC group exited no difference in alpha-diversity but significant structural change that charactered by increasing in the proportion of Lactobacillus and decreasing in Gardnerella and Atopobium after treatment.However,no significant differences in alpha-diversity and microbial structure exhibited in RVVC group(P>0.05).Conclusion The vaginal microflora existed no different structure between VVC group and RVVC group.Compared with RVVC group,the VVC group demonstrated significantly microbial structure.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2014-11-20