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[1]柳 刚,张 玲,郭晓军,等.黄芩黄素减轻脱氧胆酸对食管上皮细胞损伤机制的探讨[J].医学研究与战创伤救治(原医学研究生学报),2015,17(02):113-115，145.[doi:10.3969/j.issn.1672-271X.2015.02.001]
　LIU Gang,ZHANG Ling,GUO Xiao-jun,et al.Baicalein attenuates deoxycholate-induced injury in human esophageal epithelial cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(02):113-115，145.[doi:10.3969/j.issn.1672-271X.2015.02.001]
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黄芩黄素减轻脱氧胆酸对食管上皮细胞损伤机制的探讨()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年02期
页码:
113-115，145
栏目:
出版日期:
2015-04-22

文章信息/Info

Title:
Baicalein attenuates deoxycholate-induced injury in human esophageal epithelial cells
作者:
柳 刚1; 张 玲1; 郭晓军2; 李桂香1; 邹多武1
1.200433 上海，第二军医大学附属长海医院消化内科；2.266071 山东青岛，济南军区青岛第二疗养院干部二科
Author(s):
LIU Gang1;  ZHANG Ling1;  GUO Xiao-jun2;  LI Gui-xiang1;  ZOU Duo-wu1.
1. Department of Gastroenterology， Changhai Hospital， Second Military Medical University， Shanghai 200433， China； 2. Second Department of Gerontology， Qingdao Second Sanatorium， Jinan Military Command， Qingdao， Shandong 266071， China
关键词:
黄芩黄素; 脱氧胆酸; 食管黏膜上皮细胞; 凋亡; 炎症; 诱导性一氧化氮合酶
Keywords:
Baicalein;  deoxycholate;  human esophageal epithelial cells;  apoptosis;  inflammation;  nitric oxide
分类号:
R571；R932
DOI:
10.3969/j.issn.1672-271X.2015.02.001
文献标志码:
A
摘要:
目的 考察黄芩黄素（Baicalein）对脱氧胆酸诱导的人食管黏膜上皮细胞损伤的治疗作用。方法 原代培养人食管黏膜上皮细胞，使用脱氧胆酸（deoxycholate，500 μmol/L）进行刺激，同时给以不同浓度的黄芩黄素（1 μmol/L，10 μmol/L）进行干预治疗。12 h后，采用Western blotting和ELISA等方法观察细胞凋亡及炎症相关指标的变化。结果 黄芩黄素治疗抑制脱氧胆酸诱导的细胞凋亡，剂量依赖地降低了caspase-3剪切体的生成和Bax蛋白的表达，同时增加Bcl-2蛋白的表达；黄芩黄素亦治疗剂量依赖地抑制了脱氧胆酸诱导的炎症因子白介素8(IL-8)和巨噬细胞趋化性和激活性因子(macrophage chemoattractant protein 1，MCP-1)的生成，抑制了脱氧胆酸诱导的食管上皮细胞中诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）的蛋白表达和细胞中的一氧化氮（nitric oxide，NO）的生成。结论 黄芩黄素通过抑制炎症，抑制iNOS/NO通路，减少凋亡，从而减轻了脱氧胆酸诱导的人食管黏膜上皮细胞的损伤。
Abstract:
Objective The present study was designed to investigate whether Baicalein protected against deoxycholate-induced injury in human esophageal epithelial cells. Methods Primary cultured human esophageal epithelial cells were stimulated with deoxycholate (500 μmol/L)， and treated with Baicalein (1 μmol/L， 10 μmol/L) simultaneously. 12 hours later， the cells were collected for analysis of inflammation and apoptosis by Western blotting and ELISA. Results Baicalein treatment reduced formation of cleaved caspase-3 and protein expression of Bax and enhanced protein expression of Bcl-2， dose-dependently， indicating that Baicalein suppressed apoptosis induced by deoxycholate. Baicalein treatment suppressed deoxycholate-induced formation of pro-inflammatory cytokines including IL-8 and macrophage chemoattractant protein 1 (MCP-1). Baicalein treatment suppressed deoxycholate-induced upregulation of protein expression of inducible nitric oxide synthase (iNOS) and reduced intracellular nitric oxide (NO) levels. Conclusion Baicalein attenuated deoxycholate-induced injury in human esophageal epithelial cells， via suppressing apoptosis， inflammation， and iNOS/NO.

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备注/Memo

备注/Memo:
国家自然基金青年科学基金（81200273）
更新日期/Last Update: 2015-03-20