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[1]高文博,徐海栋,刘 刚,等.青壮年军人腰腿痛患者HLA-B27及其基因亚型检测分析[J].医学研究与战创伤救治(原医学研究生学报),2015,17(03):233-235.[doi:10.3969/j.issn.1672-271X.2015.03.003]
　GAO Wen-bo,XU Hai-dong,LIU Gang,et al.Detection of HLA-B27 and genetic subtypes in young soldiers with low back pain[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(03):233-235.[doi:10.3969/j.issn.1672-271X.2015.03.003]
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青壮年军人腰腿痛患者HLA-B27及其基因亚型检测分析()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年03期
页码:
233-235
栏目:
出版日期:
2015-05-20

文章信息/Info

Title:
Detection of HLA-B27 and genetic subtypes in young soldiers with low back pain
作者:
高文博1; 徐海栋2; 刘 刚2; 李卫巍1; 夏欣一1; 虞 伟1; 李晓军1
210002 南京军区南京总医院（南方医科大学南京临床医学院），1.临床中心实验科，2.骨科
Author(s):
GAO Wen-bo1;  XU Hai-dong2;  LIU Gang2;  LI Wei-wei1;  XIA Xin-yi1;  YU Wei1;  LI Xiao-jun1.
1.Institute of Medical Laboratory Sciences of PLA，2.Department of Orthopedics， Nanjing School of Clinical Medicine， Southern Medical University/Nanjing General Hospital of Nanjing Military Region， PLA， Nanjing， Jiangsu 210002， China
关键词:
人类白细胞抗原B27; 流式细胞术; 强直性脊柱炎; 军人; 基因分型
Keywords:
HLA-B27;  flow cytometry;  ankylosing spondylitis;  soldiers;  genetic subtypes
分类号:
R593.23
DOI:
10.3969/j.issn.1672-271X.2015.03.003
文献标志码:
A
摘要:
目的 通过检测2126例青壮年军人腰腿痛患者人类白细胞抗原B27（HLA-B27）阳性率和表达强度，了解本地区军人强直性脊柱炎（ankylosing spondylitis，AS）与HLA-B27抗原相关性，并初步探讨其对AS的临床诊断意义；通过检测确诊AS军人患者HLA-B27基因亚型，了解本地区军人HLA-B27基因亚型分布情况。方法 用流式细胞仪分析2013年8月1日-2014年1月10日2126例因腰腿痛入南京军区南京总医院就诊的军人患者HLA-B27抗原表达情况；用荧光定量PCR法对其中确诊AS患者HLA-B27基因亚型进行检测分析。结果 2126例患者中HLA-B27阳性者158例，阳性率为7.4%，男女患者HLA-B27 抗原阳性率分别为7.1%和10.0%（P＜0.01）；33例HLA-B27阳性确诊患者中，B*2704和为B*2705为主要型别，分别占51.5%和42.4%。结论 HLA-B27对于AS仅有辅助诊断作用，不宜作为AS的常规筛选指标。
Abstract:
Objective To investigate the relationship and diagnostic significance between ankylosing spondylitis and HLA-B27 antigen in soldiers in one military district by detecting the positive rate and expression intensity of HLA-B27 antigen in 2126 young soldiers with low back pain， and evaluate its clinical significance. To investigate the distribution of genetic subtypes of AS by detecting genetic subtypes of HLA-B27 in confirmed soldiers. Methods The expression of HLA-B27 antigen of peripheral blood T lymphocytes in 2126 hospital admission patients was analyzed by flow cytometry. Genetic subtypes of HLA-B27 was determined by fluorescence quantitative polymerase chain reaction assay. Results 158 patients were positive in 2126 patients. The positive rate of antigen HLA-B27 was 7.4%， and the male and female patients’ positive rates of HLA-B27 antigen were 7.1% and 10.0%， respectively (P＜0.01). Among the 33 confirmed AS patients with HLA-B27 antigen positivity， B*2704 and B*2705 were the main type with accounting for 51.5% and 42.4% respectively. Conclusion HLA-B27 is helpful for auxiliary diagnosis of AS， but is not suitable as a screening indictor of AS.

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备注/Memo

备注/Memo:
国家自然科学基金项目（81470071）
更新日期/Last Update: 2015-05-20