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[1]许永志,陈 彬,谢志雄,等.野战环境对Roche Cobas E411电化学发光免疫分析仪性能的影响[J].医学研究与战创伤救治(原医学研究生学报),2015,17(06):594-597.[doi:10.3969/j.issn.1672-271X.2015.06.010]
　XU Yong-zhi,CHEN Bin,XIE Zhi-xiong,et al.Influence of Roche Cobas E411performance under field environment[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(06):594-597.[doi:10.3969/j.issn.1672-271X.2015.06.010]
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野战环境对Roche Cobas E411电化学发光免疫分析仪性能的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年06期
页码:
594-597
栏目:
出版日期:
2015-12-15

文章信息/Info

Title:
Influence of Roche Cobas E411performance under field environment
作者:
许永志; 陈 彬; 谢志雄; 郭健莲; 徐忠玉; 夏 挺
363000 福建漳州，解放军175医院检验科
Author(s):
XU Yong-zhi;  CHEN Bin;  XIE Zhi-xiong;  GUO Jian-lian;  XU Zhong-yu;  XIA Ting.
Department of Clinical Laboratory, 175 Hospital of PLA, Zhangzhou, Fujian 363000, China
关键词:
野战环境; 医学检验装备; 性能
Keywords:
field environment;  clinical laboratory equipment;  performance
分类号:
R446.6
DOI:
10.3969/j.issn.1672-271X.2015.06.010
文献标志码:
A
摘要:
目的 探讨野战环境对Roche CCobas E411电化学发光免疫分析仪性能的影响。方法 对Roche Cobas E411系统的降钙素原（PCT）、N末端脑钠肽前体（NT-proBNP）、肌红蛋白（Mb）、高敏肌钙蛋白Ｔ（hsTNT） 4个项目在野战环境下进行精密度、正确度、回收率、线性评价，并与Roche Cobas E601电化学发光分析仪测定进行比较。结果 该仪器测定PCT的低、高水平批内变异系数（CV）分别为1.70%、0.70%，批间CV为4.09%、3.35%；NT-proBNP的批内CV为2.46%、1.61%，批间CV为5.10%、2.59%；Mb的批内CV为2.42%、1.17%，批间CV为4.71%、2.51%；hsTNT的批内CV为2.83%、1.79%，批间CV为4.77%、3.14%。Roche Cobas E411与Roche Cobas E601电化学发光分析仪测定PCT、NT-proBNP、Mb、hsTNT的结果均显著相关（r＞0.975, P＞0.05），各项目回收率均在97%~103%。Roche Cobas E411测定PCT、NT-proBNP、Mb、hsTNT的线性范围分别为(0.08～98.75) ng/mL、(7.95～33815.65) pg/mL、(26.94～2816.40) ng/mL、(5.23～9311.05) ng/L。结论 Roche Cobas E411检测PCT、NT-proBNP、Mb、hsTNT具有优异的准确性、良好的精密度和较宽的线性范围，可满足野战环境需求。
Abstract:
Objective To explore the influence of Roche Cobas E411 electrochemistry luminescence immunity analyzer (Roche Cobas E411) performance under field environment. Methods Procalcitonin (PCT), N-terminal pro-B-type natriuretic peptide (NT-proBNP), myoglobin (Mb) and high sensitive cardiac troponin T (hsTNT) detected by Roche Cobas E411 system were evaluated in precision, accuracy, recovery and linearity under field environment. The data was compared with Roche Cobas E601. Results The within-run coefficient of variations (CVs) of PCT with low and high level control using Roche Cobas E 411 analyzer were 1.70%, 0.70% respectively, whereas the between-day CVs of PCT were 4.09%, 3.35%; the within-run CVs of NT-proBNP were 2.46%, 1.61%, which showed the between-day CVs of NT-proBNP were 5.10%, 2.59%; the within-run CVs of Mb were 2.42%, 1.17%, meanwhile the between-day CVs of Mb were 4.71%, 2.51%; and the within-run CVs of hsTNT were 2.83%, 1.79%, whereas the between-day CVs of hsTNT were 4.77%, 3.14%. The level of PCT, NT-proBNP, Mb and hsTNT with Roche Cobas E411 analyzer were well related to the results on Roche Cobas E601 (r＞0.975, P＞0.05), and the recovery of these four items were all between 97% and 103%. The linear range of PCT, NT-proBNP, Mb and hsTNT were (0.08-98.75) ng/mL, (7.95-33815.65) pg/mL, (26.94-2816.40) ng/mL and (5.23-9311.05) ng/L respectively. Conclusion Roche Cobas E411 analyzer presents good accuracy, outstanding precision and wide analytical measurement range, which can meet the requirement of field environment.

参考文献/References:

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更新日期/Last Update: 2015-11-20