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[1]陈 慧,张 兰,郭以河,等.Eag1钾离子通道在卵巢癌组织中的表达及意义[J].医学研究与战创伤救治(原医学研究生学报),2016,18(01):17-20.[doi:10.3969/j.issn.1672-271X.2016.01.005]
　CHEN Hui,ZHANG Lan,GUO Yi-he,et al.Expression and significance ofether à go-go 1 channels in human ovarian cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(01):17-20.[doi:10.3969/j.issn.1672-271X.2016.01.005]
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Eag1钾离子通道在卵巢癌组织中的表达及意义()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年01期
页码:
17-20
栏目:
出版日期:
2016-01-20

文章信息/Info

Title:
Expression and significance ofether à go-go 1 channels in human ovarian cancer
作者:
陈 慧1; 张 兰1; 郭以河2; 林月丽1; 洪丽玲1
363000 福建漳州，厦门大学附属东南医院，1.妇产科，
2.病理科
Author(s):
CHEN Hui1;  ZHANG Lan1;  GUO Yi-he2;  LIN Yue-li1;  HONG Li-lin1.
1. Department of Gynaecology and Obstetrics,
2. Department of Pathology, the Affiliated Southeast Hospital of Xiamen University, Zhangzhou, Fujian 363000, China
关键词:
Eag1; 阿司咪唑; 增殖; 免疫组织化学; 卵巢癌
Keywords:
human ether à go-go1;  astemizole;  proliferation;  cell cycle;  immunohistochemistry;  ovarian cancer
分类号:
R737.31
DOI:
10.3969/j.issn.1672-271X.2016.01.005
文献标志码:
A
摘要:
目的 检测Ether à go-go 1（Eag1) 钾离子通道在人卵巢癌中的表达，对人卵巢癌细胞株SKOV3的影响及与卵巢癌患者临床特征的关系。方法 用实时定量逆转录- 聚合酶链反应 (qRT-PCR) 检测卵巢癌细胞SKOV3中Eag1的表达；用噻唑蓝比色法 (MTT) 检测Eag1钾离子通道抑制剂阿司咪唑 (Astemizole) 对SKOV3细胞增殖的影响；用流式细胞 仪检测阿司咪唑对对SKOV3细周期的影响；用蛋白印迹法 (Western blot) 和免疫组织化学的方法检测36例卵巢癌组织中Eag1蛋白的表达并结合临床病例资料进行分析。结果 阿司 咪唑可明显抑制SKOV3细胞增殖，阻滞细胞周期于G0/G1期。在卵巢癌标本中，Eag1蛋白的阳性表达率明显高于正常卵巢组织。其在不同的临床分期方面差异有统计学意义(P＜0.05) 。结论 Eag1钾离子通道异常表达于人卵巢癌组织中，并可能成为卵巢癌诊断和治疗的靶点。
Abstract:
Objective To evaluate the expression of Eag1 potassium channel in human ovarian cancer, the effects of Eag1 on human ovarian cancer cell line SKOV3 and the relationship between Eag1 expression and clinicopathological characteristics of ovarian cancer patients. Methods The expression of Eag1 in human ovarian cancer cell line SKOV3 was detected by quantificational reverse transcription polymerase chain reaction (qRT-PCR). The effects of astemizole (a inhibitor of Eag1) on SKOV3 cells proliferation and cycle were measured by MTT and ow cytometry. Then the expressions of Eag1 at protein level were detected by Western blot and immunohistochemical staining in 36 cases of ovarian cancer. The relationship between the index and clinicopathological parameters was analyzed. Results Eag1 was overexpressed in SKOV3 cells. Cell proliferation and cycle can be significantly inhibited by astemizole. Eag1 was expressed higher in the ovarian cancer specimens than in normal ovaries tissues and was associated with clinical stage (P＜0.05). Conclusion The expression of Eag1 potassium channel was aberrant in human ovarian cancer tissues and Eag1 potassium channel may represent a potential target for ovarian cancer diagnosis and treatment.

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备注/Memo

备注/Memo:
福建省漳州市自然科学计划项目（ZZ2012J25）
更新日期/Last Update: 2016-01-20