|本期目录/Table of Contents|

[1]相芳,魏玮,相红,等.牛蒡子苷对L6骨骼肌细胞中腺苷酸活化蛋白激酶信号通路的影响[J].医学研究与战创伤救治(原医学研究生学报),2016,18(02):157-159,187.[doi:10.3969/j.issn.1672-271X.2016.02.015]
　XIANG Fang,WEI Wei,XIANG Hong,et al.Effect ofarctiin on AMPK signal pathway in L6 myotubes[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(02):157-159,187.[doi:10.3969/j.issn.1672-271X.2016.02.015]
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牛蒡子苷对L6骨骼肌细胞中腺苷酸活化蛋白激酶信号通路的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年02期
页码:
157-159,187
栏目:
出版日期:
2016-04-09

文章信息/Info

Title:
Effect ofarctiin on AMPK signal pathway in L6 myotubes
作者:
相芳1; 魏玮1; 相红2; 王玉兰3; 季蔚1; 许诺1; 万小梅1
1. 210002 江苏南京，解放军81医院药剂科；2. 210000 江苏南京，南京军区第一干休所；3. 210005 江苏南京，南京军区第二干休所
Author(s):
XIANG Fang1;  WEI Wei1;  XIANG Hong2;  WANG Yu-lan3;  JI Wei1;  XU Nuo1;  WAN Xiao-mei1.
1. Department of Pharmacy, 81 Hospital of PLA, Nanjing, Jiangsu 210002, China; 2. The First Sanatorium for Retired Cadres, Nanjing Military Command, PLA, Nanjing, Jiangsu 210000, China; 3. The Second Sanatorium for Retired Cadres, Nanjing Military Co
关键词:
牛蒡子苷; 胰岛素抵抗; 腺苷酸活化蛋白激酶; L6骨骼肌细胞
Keywords:
arctiin;  insulin resistance;  AMPK;  L6 myotubes
分类号:
R966
DOI:
10.3969/j.issn.1672-271X.2016.02.015
文献标志码:
A
摘要:
目的 探讨牛蒡子苷在L6骨骼肌细胞中对葡萄糖消耗和腺苷酸活化蛋白激酶（AMP-activated protein kinase，AMPK）信号通路的调节作用。方法 用含 2％胎牛血 清的1640培养基诱导L6骨骼肌细胞分化，直至细胞生长出肌管，分化完成后的L6骨骼肌细胞用含有不同浓度牛蒡子苷的培养基培养24 h后，采用葡萄糖氧化酶法检测细胞葡萄糖消 耗，western blot法检测AMPK的亚单位AMPKα和磷酸化腺苷酸活化蛋白激酶（p-AMP-activated protein kinase，p-AMPKα）的蛋白表达水平；以real-time PCR法检测过氧化物酶 体增生物激活受体γ共激活因子1α（peroxisome-proliferator-activated receptor γ coactivator-1α，PGC-1α）mRNA表达水平。结果 牛蒡子苷浓度为1000 g/L时，可增加 L6骨骼肌细胞的葡萄糖消耗，差异显著（P＜0.01）；与对照组相比，牛蒡子苷呈浓度依赖性的增强L6骨骼肌细胞中p-AMPKα蛋白表达水平；并以相同的趋势增强PGC-1α mRNA表达 水平。结论 牛蒡子苷增加L6骨骼肌细胞的葡萄糖消耗，并激活AMPK/PGC-1α信号通路，具有潜在的改善胰岛素抵抗的作用。
Abstract:
Objective To investigate the effect of arctiin on glucose consumption and AMPK pathway in L6 myotubes. Methods L6 myoblasts were differentiated to myotubes and treated with or without arctiin. After 24 h, glucose oxidase method was performed at the end of treatment and the AMPKα and p-AMPKα levels were detected by western blot (mammalian AMPK is a trimer structure that composed of alpha, beta, gamma of three subunits, and the content detection of total AMPK and activated AMPK proteins usually refers to the alpha subunit.); The PGC-1α mRNA level were measured with real-time PCR. Results The result of glucose oxidase assay suggested that arctiin increase. Compared with control group, arctiin enhanced the activity of p-AMPKα with a concentration-dependent manner, and arctiin promoted the transcription of PGC-1α with a concentration-dependent manner, too. Conclusion Arctiin increases glucose consumption and activates AMPK/PGC-1α signal pathway in L6 myotubes, which suggests that arctiin could potentially improve insulin resistance.

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更新日期/Last Update: 2016-03-20