|本期目录/Table of Contents|

[1]刘玉,陈文琦,王平,等.炭疽杆菌双重可视化LAMP检测方法的建立[J].医学研究与战创伤救治(原医学研究生学报),2016,18(04):341-345.[doi:10.3969/j.issn.1672-271X.2016.04.002]
　LIU Yu,CHEN Wen-qi,WANG Ping,et al.Development of a duplex loop-mediated isothermal amplification assay for detection of bacillus anthracis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(04):341-345.[doi:10.3969/j.issn.1672-271X.2016.04.002]
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炭疽杆菌双重可视化LAMP检测方法的建立()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年04期
页码:
341-345
栏目:
出版日期:
2016-08-20

文章信息/Info

Title:
Development of a duplex loop-mediated isothermal amplification assay for detection of bacillus anthracis
作者:
刘玉1; 陈文琦2; 王平1; 操敏1; 韩一芳1; 张琪1; 张锦海1; 王长军1
1. 210002江苏南京，南京军区疾病预防控制中心疾控所；2. 210006江苏南京，南京医科大学附属南京第一医院皮肤科
Author(s):
LIU Yu1;  CHEN Wen-qi2; WANG Ping1;  CAO ming1; HAN Yi-fang1;  ZHANG Qi1;  ZHANG Jin-hai1; WANG Chang-jun1.
1. Center for Disease Control and Prevention of Nanjing Command,Nanjing, Jiangsu 210002, China; 2. Department of Dermatology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210016, China
关键词:
炭疽杆菌; 环介导等温扩增; 检测
Keywords:
bacillus anthracis;  loop-mediated isothermal amplification assay;  detection
分类号:
R378.72
DOI:
10.3969/j.issn.1672-271X.2016.04.002
文献标志码:
A
摘要:
目的 建立同时检测炭疽杆菌capA基因、PA基因的双重环介导等温扩增（Loop-mediated isothermal amplification,LAMP）方法，用于防范生物恐怖威胁。方法 设计和合成分别针对炭疽杆 菌capA基因、PA基因的引物对，通过优化参数，建立可同时检测capA基因、PA基因的双重LAMP方法，测试敏感性和特异性，并应用于模拟样本和实战检测。结果 双重LAMP的检测capA基因、PA基因的敏 感性均可达到50模板拷贝每反应，并具有良好的特异性，在重大保障活动中得到检验。结论 双重LAMP方法具有可以同时筛查、简单快速、灵敏度高等优点，在炭疽杆菌检测方面有良好应用前景。
Abstract:
Objective To develop a dupplex loop-mediated isothermal amplification (LAMP) assay for the detection of specific structural genes and virulence genes in bacillus anthracis. Methods Two sets of specific primers were designed and synthesized according to CapA and PA genes of bacillus anthracis. The reaction parameters were optimized to develop the dupplex LAMP assay for the rapid detection of bacillus anthracis. This study also tested the sensitivity and specificity of the LAMP assay, and applied it to test simulated and actual samples. Results A double LAMP assay for rapid detection of bacillus anthracis PA gene (plasmid pX01) and capA gene (pX02 plasmid) on visualization methods had been established. The detectable sensitivity of the duplex LAMP was 50 template copy per reaction, and it had good specificity, stability and reproducibility. Conclusion Considering LAMP’s simplicity in operation and high sensitivity, there is potential use in clinical diagnosis and surveillance of bacillus anthracis.

参考文献/References:

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相似文献/References:

[1]谭维国,陈文琦,吕 恒,等.炭疽杆菌双重实时荧光PCR检测方法的建立[J].医学研究与战创伤救治(原医学研究生学报),2013,15(06):556.[doi:10.3969/j.issn.1672-271X.2013.06.002]
　TAN Wei-guo,CHEN Wen-qi,LV Hen,et al.Development of a duplex real-time PCR assay for detection of bacillus anthracis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(04):556.[doi:10.3969/j.issn.1672-271X.2013.06.002]

备注/Memo

备注/Memo:
国家科技重大专项（2013ZX10004103004）；全军十二五重点项目（AWS11C001，AWS11C009）；军队后勤科研重点项目（BWS14J025）；国家自然科学基金青年基金（81402619）；江苏省科技 支撑计划社会发展项目（BE2013603）；江苏省自然科学基金青年基金（BK20130083）
更新日期/Last Update: 2016-07-20