|本期目录/Table of Contents|

[1]李辉,赵宇蕾,周国华,等.HPLC法测定人血清伏立康唑浓度[J].医学研究与战创伤救治(原医学研究生学报),2017,19(01):30-33.[doi:10.3969/j.issn.1672-271X.2017.01.008]
　LI Hui,ZHAO Yu-lei,ZHOU Guo-hua,et al.The determination of voriconazole concentration in human serum by HPLC[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(01):30-33.[doi:10.3969/j.issn.1672-271X.2017.01.008]
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HPLC法测定人血清伏立康唑浓度()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年01期
页码:
30-33
栏目:
出版日期:
2017-01-20

文章信息/Info

Title:
The determination of voriconazole concentration in human serum by HPLC
作者:
李辉; 赵宇蕾; 周国华; 芮建中
作者单位：210002南京，南京军区南京总医院药理科
Author(s):
LI Hui;  ZHAO Yu-lei;  ZHOU Guo-hua;  RUI Jian-zhong
(Department of Pharmacology, Nanjing General Hospital of Nanjing Military Region, PLA, Nanjing 210002, Jiangsu, China)
关键词:
伏立康唑; 高效液相色谱; 血清浓度
Keywords:
Voriconazole;  High Performance Liquid Chromatography;  Serum concentration
分类号:
R969.3
DOI:
10.3969/j.issn.1672-271X.2017.01.008
文献标志码:
A
摘要:
目的 建立高效液相色谱（high performance liquid chromatography，HPLC）测定人血清中伏立康唑的方法 ，为临床伏立康唑的个体化用药提供方法 学基础。方法 采用7%高氯酸直接沉淀血清蛋白，色谱柱采用汉邦Hedera ODS-2（4.6 μm×250 mm，5 μm），流动相为乙腈-20 mmol/L磷酸二氢钠缓冲液（pH=6.0）（60∶40，v/v），流速为1.0 mL/min，检测波长为254 nm。结果 伏立康唑在0.367～23.5 μg/mL间线性良好（r=0.9964），批内、批间准确度偏差＜±5.0%，批内、批间精密度相对标准偏差（relative standard deviation，RSD）＜11.1%，在室温放置、冰冻、反复冻融条件下考察样品稳定性，伏立康唑均保持稳定，偏差＜±12.5%。结论 建立的HPLC测定人血清伏立康唑浓度的方法 具有操作简便、特异性高、灵敏度高、成本低的特点，更适合临床中常规应用。
Abstract:
Objective To establish an high performance liquid chromatography (HPLC) method to determine the voriconazole concentration in human serum, that will provide methodological foundation for individual medicine.Methods After the precipitation of serum proteins with 7% perchloric acid, the voriconazole concentration was determined by HPLC on a reversed phase Hedera ODS-2 column (4.6 μm×250 mm, 5 μm). The mobile phase was a mixture of acetonitrile and 20 mmol/L sodium dihydrogen phosphate buffer adjusted to pH 6.0, with the mix ratio 60∶40, and was delivered at a flow rate of 1.0 mL/min. The UV detection was set at 254 nm.Results The peak area for voriconazole was linearly related to its concentrations, which ranged from 0.367 to 23.5 μg/mL (r=0.9964). The intra- and inter-assay variations were within ±5.0% and their relative standard deviation values were within 11.1%. The serum samples were stable when stored at room temperature, after frozen for 15 days and after 3 cycles of freeze and thaw processes; deviations were within ±12.5%.Conclusion The established HPLC method is simple and fast with high specificity, high sensitivity and low cost. The improved method is more suitable for clinical routine use

参考文献/References:

[1]Keller P, Muller C, Engelhardt I, et al. An antifungal benzimidazole derivative inhibits ergosterol biosynthesis and reveals novel sterols[J]. Antimicrob Agents Chemother, 2015, 59(10): 6296-6307.
[2]Sharifzadeh A, Soltani HM. Shokri. Evaluation of virulence factors and antifungal susceptibility patterns of different Candida species isolated from the female camel (Camelus dromedarius) genital tract[J]. Mycoses, 2015, 58(8): 478-84.
[3]Liu PD，Mould R. Population pharmacokinetic-pharmacodynamic analysis of voriconazole and anidulafungin in adult patients with invasive aspergillosis[J]. Antimicrob Agents Chemother, 2014, 58(8): 4727-4736.
[4]Jin H, Wang T, Falcione BA, et al. Trough concentration of voriconazole and its relationship with efficacy and safety: a systematic review and meta-analysis[J]. J Antimicrob Chemother, 2016, 71(7): 1772-1785.
[5]Wang T, Xie J, Wang Y, et al. pharmacokinetic and pharmacodynamic properties of oral voriconazole in patients with invasive fungal infections[J]. Pharmacotherapy, 2015, 35(9): 797-804.
[6]Miyakis S, van Hal SJ, Solvag C J, et al. Clinician ordering practices for voriconazole therapeutic drug monitoring: experiences of a referral laboratory[J]. Ther Drug Monit, 2010, 32(5): 661-664.
[7]于淼,原源. HPLC法测定益心酮片中四种黄酮类成分的含量[J]. 东南国防医药, 2015,17(2): 157-159,165.
[8]费燕, 林秀丽, 胡永狮, 等. 柱前衍生-HPLC法测定人血清中丙戊酸钠的方法改进[J]. 东南国防医药, 2010, 12(5): 418-419,426.
[9]崔恩忠,唐安福,刘文雅,等. 高效液相色谱法测定益肾丸中特女贞苷的含量[J]. 医学研究生学报,2015,28(1):78-81.
[10]汤淏,崔恩忠,唐安福,等. 高效液相色谱-蒸发光散射法测定气血双补口服液中黄芪甲苷的含量[J]. 医学研究生学报,2013,26(3):287-289.
[11]Chawla, PK, Dherai AJ, Ashavaid TF. Plasma voriconazole estimation by HPLC[J]. Indian J Clin Biochem, 2016, 31(2): 209-14.
[12]Guinea J, Escribano P, Marcos-Zambrano LJ, et al. Therapeutic drug monitoring of voriconazole helps to decrease the percentage of patients with off-target trough serum levels[J]. Med Mycol, 2016, 54(4): 353-360.
[13]黄娟, 冯仕银, 杜晓琳, 等. HPLC法测定人血浆中伏立康唑浓度[J]. 解放军药学学报, 2013,29(1): 32-34.
[14]王超,张弋. RP-HPLC法测定肾移植术后患者血浆中伏立康唑的浓度[J]. 中国药房, 2014,25(18): 1668-1670.
[15]雷玲. 高效液相色谱检测血浆伏立康唑浓度研究[J]. 医学综述, 2014,20(19): 3646-3648

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-01-20