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[1]徐晓峰,许秀娟,郝建,等.高温气体吸入致豚鼠急性肺损伤动物模型制备的实验研究[J].医学研究与战创伤救治(原医学研究生学报),2017,19(05):449-452.[doi:10.3969/j.issn.1672-271X.2017.05.001]
　XU Xiao-feng,XU Xiu-juan,HAO Jian,et al.An experimental study of acute lung injury in guinea pigs induced by high temperature gas[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(05):449-452.[doi:10.3969/j.issn.1672-271X.2017.05.001]
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高温气体吸入致豚鼠急性肺损伤动物模型制备的实验研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年05期
页码:
449-452
栏目:
出版日期:
2017-09-29

文章信息/Info

Title:
An experimental study of acute lung injury in guinea pigs induced by high temperature gas
作者:
徐晓峰1; 许秀娟1; 郝建1; 等
作者单位：1.310007杭州, 南京军区杭州疗养院心肺康复医学中心；2.310034杭州, 解放军第117医院急诊科
Author(s):
XU Xiao-feng1;  XU Xiu-juan1;  HAO Jian1;  WU Sheng1;  LI Shu-wen1;  QIAN Jun1;  CHANG Yue-jin1;  LIU Juan1;  WANG Li-jun1;  YAO Yuan2
（1. Cardiopulmonary Rehabilitation Medicine Centre, Hangzhou Sanatorium of Nanjing Military Region, Hangzhou 310007, Zhejiang, China；2. Department of Emergency, the 117th Hospital of PLA, Hangzhou 310034, Zhejiang, China）
关键词:
实验动物模型; 急性肺损伤; 豚鼠
Keywords:
Experimental animal model; Acute lung injury; Guinea pig
分类号:
R563.8
DOI:
10.3969/j.issn.1672-271X.2017.05.001
文献标志码:
A
摘要:
目的 制备高温气体吸入致豚鼠急性肺损伤（ALI）动物模型。方法 选择20只雄性豚鼠随机分为2组，每组10只，其中健康豚鼠为对照组、急性肺损伤豚鼠为实验组。实验组吸入150℃高温气体制作豚鼠ALI动物模型，然后处死2组豚鼠并取豚鼠右肺的大体标本，称重计算豚鼠肺湿/干重比（W/D），选取2组豚鼠左上肺组织进行HE染色，利用光学显微镜观察肺组织病理变化情况，及时记录其病理变化的结果 ；对豚鼠动脉血液进行血气分析并比较。结果 实验组豚鼠肺组织病理结构呈现明显的急性炎症性改变;实验组豚鼠肺W/D比值显著高于对照组[（5.72±0.31） vs （4.88±0.11）,P＜0.05];实验组豚鼠pH、PaO2显著低于对照组[（7.02±0.12） vs （7.38±0.04），（98.80±62.60）mmHg vs （177.30±24.70）mmHg，P＜0.05]，而PaCO2显著高于对照组[（74.70±23.23）mmHg vs （31.10±8.77）mmHg，P＜0.05]。结论 成功制作豚鼠ALI实验动物模型。该模型达到ALI要求，仿真性能高、重复性好、稳定性高，为今后研究高温气体吸入致ALI的临床及基础研究提供了可靠的动物模型。
Abstract:
Objective To prepare the experimental animal models of acute lung injury (ALI) induced by high temperature gas inhalation in guinea pigs.Methods Twenty male guinea pigs were randomly divided into 2 groups, each group of 10 guinea pigs. The healthy guinea pigs were set as the control group, and the ALI guinea pigs were set as the experimental group. High temperature gas (150℃) was given to the guinea pigs of the experimental group for inhaling 5 minutes to induce the animal model of ALI. Then the two groups of guinea pigs were executed and lung specimens were taken from guinea pigs. Wet/dry weight ratio (W/D) of the right lungs was calculated. Up-left lungs of the two groups were stained with HE, and the pathological changes of lung tissues were observe by optical microscope in the two groups; The blood gas of the guinea pigs arterial blood was also analyzed and compared.Results The pathological structure of lung tissue of the experimental group showed obvious acute inflammatory changes. The lung W/D ratio of guinea pigs inthe experimental group was significantly higher than that in the control group [（5.72±0.31） vs （4.88±0.11）,P＜0.05]. The pH and PaO2 of guinea pigs in the experimental group were significantly lower than those in the control group[（7.02±0.12） vs （7.38±0.04），（98.80±62.60）mmHg vs （177.30±24.70）mmHg，P＜0.05], while PaCO2 was significantly higher than that in the control group [（74.70±23.23）mmHg vs （31.10±8.77）mmHg，P＜0.05]. Conclusion The high temperature gas supplied to guinea pigs designed in this study can successfully establish ALI experimental animal model which meets the requirement of ALI and has high simulation performance, good repeatability, high stability.It provides a reliable animal model for the clinical and basic research of ALI induced by high temperature gas inhalation.

参考文献/References:

[1]陈娇, 钱晓明, 聂时南. 急性肺损伤动物模型的研究现状[J]. 医学研究生学报, 2013, 26(8): 851-854.
[2]Fanelli V, Ranieri VM. Mechanisms and clinical consequences of acute lung injury[J]. Ann Am Thorac Soc, 2015,12（Suppl 1）: S3-S8.
[3]王婷, 梁华平, 柴鉴深, 等. 三种大鼠急性肺损伤模型的比较[J]. 成都医学院学报, 2016,11(1): 5-9.
[4]霍然, 胡源, 李元洲. 建筑火灾安全工程导论[M]. 合肥: 中国科学技术大学出版社, 1999: 78-79.
[5]Mireles-Cabodevila E, Chatburn RL, Thurman TL, et al. Application of mid-frequency ventilation in an animal model of lung injury: a pilot study[J]. Respir Care, 2014,59(11): 1619-1627.
[6]Fang Y, Xu P, Gu C, et al. Ulinastatin improves pulmonary function in severe burn-induced acute lung injury by attenuating inflammatory response[J]. J Trauma, 2011, 71(5): 1297-1304.
[7]陈魁君, 王建民. 细胞因子生物标志物在爆炸致急性肺损伤检测中的应用[J]. 中华诊断学电子杂志, 2016, 4(1): 19-22.
[8]潘蔚, 刘军生, 刘润生, 等. 急性肺损伤/急性呼吸窘迫综合征致肺水肿的病理生理研究进展[J]. 解放军预防医学杂志, 2016, 34(4): 576-579.
[9]孙红双, 吕菁君, 魏捷. 急性肺损伤发病机制及治疗的未来研究趋势[J]. 医学研究杂志,2016,45(1):181-183.
[10]Zhang Y, Zhang B, Xu DQ, et al. Tanshinone ⅡA attenuates seawater aspiration-induced lung injury by inhibiting macrophage migration inhibitory factor [J]. Biol Pharm Bull, 2011, 34（7）: 1052-1057.
[11]李鲁欢, 张友琴，郝建, 等. 丹参酮IIA磺酸钠治疗爆炸豚鼠急性肺损伤实验研究[J]. 临床肺科杂志, 2015, 20(11): 1991-1994.
[12]常月进, 吴升, 郝建, 等.不同剂量乌司他丁对高温气体致豚鼠急性肺损伤的影响[J]. 临床肺科杂志, 2011, 17(5): 781-783.
[13]Han S, Mallampalli RK. The acute respiratory distress syndrome: from mechanism to translation[J]. J Immunol, 2015, 194(3): 855-860.
[14]刘娟, 吴升, 郝建, 等. 丹参酮ⅡＡ磺酸钠治疗火器伤致豚鼠急性肺损伤与细胞因子调节作用的研究[J]. 中华临床医师杂志（电子版）, 2013, 7(6): 150-153.
[15]黄海嵘, 张雷,李德闽, 等. 大鼠体外循环后肺损伤的实验研究[J]. 东南国防医药, 2014, 16(2): 113-116.
[16]黄文彬, 申捷, 张琳, 等. 乌司他丁对鼠急性肺损伤的保护作用及相关因子的表达[J]. 中华急诊医学杂志, 2010, 19(1): 37-42.
[17]Menezes LG, Uzuelli JA, Tefé-Silva C, et al. Acute lung injury induced by the intravenous administration of cigarette smoke extract[J]. J Bras Pneumol, 2013, 39(1): 39-47.

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备注/Memo

备注/Memo:
基金项目：全军医药卫生科研基金（10Z034）
更新日期/Last Update: 2017-09-20