|本期目录/Table of Contents|

[1]王华涛,张勇,王志颐,等.相关炎症因子在声带黏膜损伤中的作用[J].医学研究与战创伤救治(原医学研究生学报),2017,19(05):453-456.[doi:10.3969/j.issn.1672-271X.2017.05.002]
　WANG Hua-tao,ZHANG Yong,WANG Zhi-yi,et al.The role of related inflammatory factors in vocal mucosa damage[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(05):453-456.[doi:10.3969/j.issn.1672-271X.2017.05.002]
点击复制

相关炎症因子在声带黏膜损伤中的作用()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年05期
页码:
453-456
栏目:
出版日期:
2017-09-29

文章信息/Info

Title:
The role of related inflammatory factors in vocal mucosa damage
作者:
王华涛1; 张勇2; 王志颐2; 江满杰2; 吴明海2; 潘晗2; 程友 2; 陈伟2
作者单位：1.223200淮安，淮安市淮安医院耳鼻咽喉头颈外科；2.210002南京，南京军区南京总医院耳鼻咽喉头颈外科
Author(s):
WANG Hua-tao1; ZHANG Yong2;  WANG Zhi-yi2;  JIANG Man-jie2;  WU Ming-hai2;  PAN Han2;  CHENG You2;  CHEN Wei2
（1.Department of Otolaryngology-Head and Neck Surgery, Huaian Hospital，Huaian 223200,Jiangsu,China;2.Department of Otolaryngology-Head and Neck Surgery, Nanjing General Hospital of Nanjing Military Region，PLA, Nanjing 210002, Jiangsu, China）
关键词:
声带白斑; 咽喉反流; 炎症; 炎症因子
Keywords:
Vocal leukoplakia;  Laryngopharyngeal reflux;  Inflammation;  Inflammatory factor
分类号:
R764
DOI:
10.3969/j.issn.1672-271X.2017.05.002
文献标志码:
A
摘要:
目的 通过观察相关炎症因子在不同程度炎症声带黏膜表达的差异，探讨其对声带黏膜屏障损伤的作用。方法 将26例确诊伴有咽喉反流(LPR)的声带白斑且拟行手术治疗的患者随机分为质子泵抑制剂(PPI)组（口服艾司奥美拉唑镁肠溶片）和对照组（口服胃黏膜保护剂），每组13例，分别采集手术中及术后8周的声带黏膜标本。用HE染色对声带炎症的病理改变进行评分比较；ELISA法测定炎症因子TNF-α、IL-1β、IL-6在不同程度炎症黏膜的表达水平。所有患者每月定期复查，随访时间至少为12个月。结果 术后12个月随访，对照组和PPI组累计复发率分别为84.6％和38.5％，累计复发率差异有统计学意义（P<0.05）。PPI组术后8周声带黏膜炎症病理评分均值较术中黏膜评分明显降低（P<0.01）；对照组术中声带黏膜炎症病理评分均值与术后8周评分均值相比，差异无统计学意义（P>0.05）。ELISA检测显示，PPI组术后8周声带黏膜IL-1β、TNF-α、IL-6表达与术中比较显著下调（P<0.01）；而对照组术中及术后8周声带黏膜IL-1β、TNF-α、IL-6表达差异无统计学意义（P>0.05）。结论 咽喉反流物刺激声带黏膜的炎症反应可能是声带黏膜损伤的重要机制。
Abstract:
Objective To study the roles of related inflammatory factors in vocal cord mucosal barrier damage via probing the differential expression of these factors in vocal cord mucosa with inflammation of different degrees.Methods Twenty-six patients suffering from vocal leukoplakia accompanied with laryngopharyngeal reflux (LPR) were randomly divided into groups with or without oral administration of proton pump inhibitor (PPI), 13 cases in each group. Mucosal specimens of vocal cord were collected from all the patients during operation and at 8 weeks after operation. HE staining was used to score the pathological changes of the mucosa. ELISA was utilized to detect the levels of inflammatory factors including TNF-α, IL-1 and IL-6.Results The accumulated recurrence rates at 12 months after operation of PPI-treated group and the control group were 38.5% and 84.6% respectively, and the difference was statistically significant (P<0.05). In the the control group, the average inflammation score evaluated by HE staining in specimens obtained 8 weeks after operation was significantly lower than those obtained during operation, and the difference was statistically significant (P<0.01). In the control group, the difference was statistically insignificant (P>0.05). As for IL-1β, TNF-α and IL-6, their expression in the specimens of 8 weeks after operation in the PPI-treated group were down-regulated when com-pared with those in the specimens obtained during operation (all P<0.05), while their differences in the control group were statistically insignificant (all P>0.05).Conclusion The possible mechanism of vocal mucosal barrier damage in vocal leukoplakia accompanied with LPR maybe the vocal mucosal inflammation induced by refluxed materials.

参考文献/References:

[1]陈伟, 季寒, 吴明海，等. 抑酸治疗对声带白斑术后相关症状的影响[J]. 医学研究生学报，2017，30(1): 66-69.
[2]Hayden MS, Ghosh S. NF-kappa B，the first quarter-century: remarkable progress and outstanding questions[J]. Genes Dev, 2012,26(3): 203-234.
[3]李湘平, 黄柞峰, 吴婷, 等. 咽喉反流在声带白斑及早期声带癌发病中的初步观察[J]. 中华耳鼻咽喉头颈外科杂志，2014，49(5): 362-367.
[4]Liu W, Konermann A, Guo T, et al. Canonical Wnt signaling differently modulates osteogenic differentiation of mesenchymal stem cells derived from bone marrow and from periodontal ligament under inflammatory conditions[J]. Biochim Biophys Acta, 2014,1840(3):1125-1134.
[5]Oberg F, Haseeb A, Ahnfelt M, et al. Herbal melanin activates TLR4/NF-kB signaling pathway[J]. Phytomedicine, 2009, 16(5):477-484.
[6]Basak S, Shih VF, Hoffmann A. Generation and activation of multiple dimeric transcription factors within the NF-kappa B signaling system[J]. Mol Cell Biol, 2008,28(10):3139-3150.
[7]Ramakrishna G, Rastogi A, Trehanpati N, et al. From cirrhosis to hepatocellular carcinoma: new molecular insights on inflammation and cellular senescence[J]. Liver Cancer，2013，2(3-4): 367-383.
[8]Nikolaou K, Sarris M, Talianidis I. Molecular pathways: the complex roles of inflammation pathways in the development and treatment of liver cancer[J]. Clin Cancer Res, 2013,19(11): 2810-2816.
[9]Umar A, Steele VE, Menter DG, et al. Mechanisms of nonsteroidal anti-inflammatory drugs in cancer prevention[J]. Semin Oncol，2016，43(1): 65-77.
[10]Fan Y, Mao R, Yang J. NF-κB and STAT3 signaling pathways collaboratively link inflammation to cancer[J]. Protein Cell，2013，4(3): 176-85.
[11]Tai DI, Tsai SL, Chang YH, et al. Constitutive activation of nuclear factor kappa B in hepatocellular carcinoma[J]. Cancer, 2000,89(11): 2274-2281.
[12]Isenberg JS, Crozier DL, Dailey SH. Institutional and comprehensive review of laryngeal leukoplakia[J]. Ann Otol Rhinol Laryngol, 2008, 117(1): 74-79.
[13]Uray IP, Dmitrovsky E, Brown PH. Retinoids and rexinoids in cancer prevention: from laboratory to clinic[J]. Semin Oncol，2016，43(1): 49-64.
[14]庄佩耘, 周莉, 郝玲，等. 反流性喉炎与声带白斑关系的初步探讨[J]. 听力学及言语疾病杂志, 2010，18(5): 448-452.

相似文献/References:

备注/Memo

备注/Memo:
基金项目：江苏省自然科学基金（BK20161388）
更新日期/Last Update: 2017-09-20