|本期目录/Table of Contents|

[1]张杰,高波.UVB诱导人皮肤成纤维细胞的SMP30基因表达变化[J].医学研究与战创伤救治(原医学研究生学报),2018,20(02):126-129.[doi:10.3969/j.issn.1672-271X.2018.02.004]
　ZHANG Jie,GAO Bo.Study on the change of SMP30 in HSF with induced by UVB[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2018,20(02):126-129.[doi:10.3969/j.issn.1672-271X.2018.02.004]
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UVB诱导人皮肤成纤维细胞的SMP30基因表达变化()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第20卷
期数:
2018年02期
页码:
126-129
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
Study on the change of SMP30 in HSF with induced by UVB
作者:
张杰;  高波
作者单位：441000襄阳，襄阳市襄州区人民医院皮肤科（张杰）；442000十堰，十堰市太和医院检验科（高波）
Author(s):
ZHANG Jie1; GAO Bo2
(1.Department of Dermatology，Xiangzhou District People′s Hospital，Xiangyang 441000,Hubei,China;2.Department of Laboratory Medicine, Taihe Hospital, Shiyan 442000,Hubei,China)
关键词:
人皮肤成纤维细胞; 紫外线中波; 衰老标记蛋白30; 四唑盐比色法; 实时荧光定量PCR
Keywords:
human skin fibroblast;  ultraviolet B;  senescence marker protein30;  MTT;  FQ-PCR
分类号:
R334.5
DOI:
10.3969/j.issn.1672-271X.2018.02.004
文献标志码:
A
摘要:
目的 研究经过不同剂量紫外线中波(UVB)照射不同时间后正常人皮肤成纤维细胞(HSF)中衰老标记蛋白30(SMP30)基因的表达改变，探讨UVB诱导HSF衰老可能的机制。方法 以未采用UVB照射的HSF为对照组，经过不同剂量UVB(100、200、300mJ/cm2)处理不同时间(1、2、3d后)的HSF为实验组，四唑盐比色法(MTT)观察HSF的生长活性，流式细胞仪检测HSF的凋亡率，实时荧光定量PCR技术(FQ-PCR)检测实验组和对照组中SMP30基因的表达。结果 不同照射时间组和不同剂量组之间，随着UVB照射时间延长和剂量的增加，HSF细胞增殖抑制率逐渐增高，细胞的凋亡率也逐渐增加，差异有统计学意义(P＜0.05)； UVB处理后实验组中SMP30基因的相对表达水平(0.66±0.19)明显低于对照组(1.15±0.11)，差异有统计学意义(P＜0.01)；SMP30基因的表达水平随着UVB作用的时间和剂量的不同而变化，呈明显的时间依赖性和浓度剂量依赖性，不同照射时间组和不同剂量组之间基因表达的差异有统计学意义(P＜0.05)。结论 经过不同剂量UVB处理不同时间后可以抑制HSF细胞生长，促进细胞凋亡，大剂量长时间UVB照射可诱导HSF发生衰老改变。
Abstract:
Objective To explore the expression of SMP30 in human skin fibroblast (HSF) after different dose and time treatments with ultraviolet B(UVB), and to study the mechanism of UVB to induce HSF senescence. Methods HSF was set as the control group and different dose (100, 200, 300 mJ/cm2) of UVB treatment of different time (1, 2, 3 d) after the HSF was set as the experimental group. FQ-PCR was used to test the expression of SMP30 gene in two groups. Results HSF treated with UVB displayed a slow growth in comparison with the control group. In the different irradiation time group and different dose groups, it showed that with the increasing of UVB irradiation time and dose, the cellular proliferation inhibition rate and the cell apoptosis rate increased gradually. The difference was statistically significant (P＜0.05). The expression level of SMP30 gene in the experimental group (0.66±0.19) was significantly lower than that in the control group (1.15±0.11), the difference was statistically significant (P＜0.01). The expression level of the genes varied with the time and dose of UVB, and it had obvious time-dependent and concentration-dependent. Differences in gene expression between different exposure time groups and different dose groups were statistically significant (P＜0.05). Conclusion Different concentrations of UVB can inhibit HSF cell growth, promote apoptosis, and large doses of UVB irradiation can induce HSF to be senescence.

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备注/Memo

备注/Memo:
基金项目：襄阳市科技计划项目(2013-8-1709)
更新日期/Last Update: 2018-03-20