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[1]李萍,罗雪,何根林,等.热应激对小鼠脾淋巴细胞活性及炎性基因表达的影响[J].医学研究与战创伤救治(原医学研究生学报),2019,21(02):113-118.[doi:10.3969/j.issn.1672-271X.2019.02.001]
　LIPing,LUOXue,HEGen-lin,et al.Effects of heatstroke on splenic lymphocyte activity and expression of inflammatory genes in mice[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(02):113-118.[doi:10.3969/j.issn.1672-271X.2019.02.001]
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热应激对小鼠脾淋巴细胞活性及炎性基因表达的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年02期
页码:
113-118
栏目:
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of heatstroke on splenic lymphocyte activity and expression of inflammatory genes in mice
作者:
李萍; 罗雪; 何根林; 张晓亮; 罗珍; 申婷婷; 杨举; 李文晶; 刘军; 王宫; 杨学森
作者单位：400038重庆，陆军军医大学军事预防医学系热带医学教研室（李 萍、罗 雪、何根林、张晓亮、罗 珍、申婷婷、杨 举、李文晶、刘 军、王 宫、杨学森）
Author(s):
LI　Ping;  LUO　Xue;  HE　Gen-lin;  ZHANG　Xiao-liang;  LUO　Zhen;  SHEN　Ting-ting;  YANG　Ju;  LI　Wen-jing;  LIU　Jun;  WANG　Gong;  YANG　Xue-sen
（Department of Tropical Medicine,College of Military Preventive Medicine,Army Medical University,Chongqing 400038, China）
关键词:
热应激; 炎症因子; 脾; 淋巴细胞; CD69
Keywords:
heat stress;  inflammatory factors;  spleen;  lymphocytes;  CD69
分类号:
R134.3
DOI:
10.3969/j.issn.1672-271X.2019.02.001
文献标志码:
A
摘要:
目的　热射病是病死率较高的致命性急症。系统性炎症反应综合征是中暑发生发展过程中的一个重要环节。作为机体最大的淋巴器官，文章研究将探索热应激对于脾脏功能的影响。 方法　体内实验中，将66只健康雄性BALB/c小鼠随机分为对照组以及热应激0 h、1 h、2 h、6 h、12 h、20 h、24 h、3 d、5 d、7 d组，每组6只。对照组小鼠不受热应激。热应激组小鼠于人工环境模拟舱进行加热，当直肠温度达到41.5 ℃时，核心温度维持在（41.5℃±0.2）℃，持续30 min。热应激后，各组小鼠分别在不同时间点观察体重、脾重量、脾系数的变化。体外实验中，9只12周龄健康雄性BALB/c小鼠于无菌条件下摘除脾，分离出小鼠原代脾淋巴细胞。原代淋巴细胞分别采用37 ℃（37 ℃组，作为对照）和42 ℃（42 ℃组，热应激）进行培养。实时定量聚合酶链反应（PCR）法检测IL-6、IL-10、MCP-1、IFN-γ、TNF-α、IL-12p70的表达变化。采用流式细胞仪检测细胞表面CD69的表达。 结果　与对照组比较，除12 h和5 d组之外，各热应激组小鼠Δ重量显著下降（P<0.05），热应激可致动物出现明显的脱水现象。体外实验显示，42 ℃组小鼠脾原代淋巴细胞中IFN-γ、IL-10、IL-6、MCP-1、TNF-α和IL-12p70基因的表达水平较37 ℃组显著增加（P<0.05），并且还能促进CD69的表达（P<0.01）。 结论　热应激对脾功能具有促进作用，能够增强脾淋巴细胞的炎症因子的基因以及CD69表达，为阐释热射病所致全身炎症反应的可能机制提供了基础参考。
Abstract:
Objective　Heat stroke is a fatal disease with high mortality.Systemic inflammatory response syndrome is a key process in the development of heatstroke.As the largest lymph organ of the body,this study will explore the effect of heatstroke on the function of the spleen.　Methods　In vivo,randomized 66 healthy male BALB/c mice into control groups and heat stress 0 h，1 h，2 h，6 h，12 h，20 h，24 h，3 d，5 d，7 d group,six in each group.The mice in the control group were not subjected to heat stress，random grouping of male BALB/c mice were exposed to heat in an environment-controlled chamber.When the rectal temperature reaches 41.5 ℃,the core temperature was maintained at （41.5±0.2）℃ for 30 minutes.After heat stress,the changes of body weight,spleen weight and spleen coefficient were observed at different time points.In vitro,spleen were removed from the mice under sterile conditions and the primary splenic lymphocytes were isolated from mice.Primary lymphocytes were cultured at 37 ℃ (37 ℃ group，control) and 42 ℃ (42 ℃ group，heat stress) respectively.Real time quantitative polymerase chain reaction (PCR) was used to detect the expression changes of IL-6,IL-10,MCP-1,IFN-γ,TNF-α and IL-12p70.The expression of CD69 on the cell surface was detected by flow cytometry.　Results　In contrast with the control group,the weight of heat stress mice was significantly reduced except for the 12 h and 5 d groups（P<0.05）.Heat stress can lead to noticeable dehydration in mice.In vitro,heat stress could significantly increase the expression of IFN- γ,IL-10,IL-6,MCP-1,TNF-α,and IL-12p70 genes in the spleen primary lymphocytes of mice（P<0.05）,and also promote the expression of CD69（P<0.01）.　Conclusion　Heat stress can promote the spleen function and enhance the gene of inflammatory factors and the expression of CD69 in spleen lymphocytes.Our study provides a laboratory reference for the explanation of the possible mechanism of systemic inflammation induced by heatstroke.

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备注/Memo

备注/Memo:
基金项目：基金项目:国家自然科学基金（81302413）
更新日期/Last Update: 2019-03-20