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[1]彭俊,刘英杰,宗阳,等.miR?125b调控Runx2/Osx表达对骨髓间充质干细胞成骨机制的影响[J].医学研究与战创伤救治(原医学研究生学报),2019,21(02):124-129.[doi:10.3969/j.issn.1672-271X.2019.02.003]
　PENGJun,LIUYing-jie,ZONGYang,et al.Effect of miR-125b regulating the expression of Runx2/Osx on osteogenesis mechanism of bone mesenchymal stem cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(02):124-129.[doi:10.3969/j.issn.1672-271X.2019.02.003]
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miR?125b调控Runx2/Osx表达对骨髓间充质干细胞成骨机制的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年02期
页码:
124-129
栏目:
基础研究
出版日期:
2019-03-20

文章信息/Info

Title:
Effect of miR-125b regulating the expression of Runx2/Osx on osteogenesis mechanism of bone mesenchymal stem cells
作者:
彭俊; 刘英杰; 宗阳; 詹玉林
作者单位：201306 上海，上海健康医学院附属第六人民医院东院骨科（彭 俊、刘英杰、宗 阳、詹玉林）
Author(s):
PENG　Jun;  LIU　Ying-jie;  ZONG　Yang;  ZHAN　Yu-lin
（Department of Orthopedics, Eastern Hospital of Shanghai Sixth People’s Hospital Affiliated to Shanghai University of Medicine & Health Sciences,Shanghai 201306,China）
关键词:
miR?125b; Osx; 骨髓间充质干细胞; 成骨; 机制
Keywords:
miR-125b;  Osx;  bone marrow mesenchymal stem cells;  osteogenesis;  mechanism
分类号:
R392.12
DOI:
10.3969/j.issn.1672-271X.2019.02.003
文献标志码:
A
摘要:
目的　分析miR-125b调控成骨细胞特异性转录因子Runx2/Osx表达对骨髓间充质干细胞（BMSCs）成骨机制的影响。 方法　40只雌性大鼠随机分为对照组、骨折模型组、骨折模拟物组、骨折抑制剂组各10只，建立大鼠股骨干闭合骨折模型（对照组除外），造模后分离培养BMSCs，7 d后骨折模拟物组转染miRNA-125b模拟物（mimic），骨折抑制剂组转染miRNA-125b 抑制剂（inhibitor），观察BMSCs形态学、表达及增殖情况，应用Western blot法检测成骨基因[骨钙素（OCN）骨桥蛋白(OPN)、Osx、Runx2蛋白]、成脂基因[过氧化物酶体增殖物激活受体(PPARγ)]的表达。　结果　qPCR显示miR-125b在骨折模型BMSCs中表达上调[（1.48±0.18） vs （0.95±0.12），P<0.05]；转染7 d时，骨折抑制剂组细胞质出现红染脂滴细胞，骨折模拟物组在第14天时出现一定脂肪细胞形态特征。骨折模拟物组Runx2、OCN、Osx分别为（0.20±0.03）、（0.45±0.05）、（0.34±0.04），均低于其他3组，而PPARγ（0.51±0.05）高于其他3组，骨折模拟物组OPN（0.52±0.06）低于骨折抑制剂组（0.88±0.02），差异有统计学意义（P<0.05），而与对照组、骨折模型组比较差异无统计学意义（P>0.05）。　结论　miR-125b可通过调控Runx2/Osx而负性调节OCN表达，正性调节PPARγ、OPN的表达，从而调控BMSCs成骨能力。
Abstract:
Objective　To analyze the effect of miR-125b regulating the expression of Runx2/Osx on osteogenesis mechanism of bone mesenchymal stem cells (BMSCs).　Methods　Forty female rats were randomly divided into control group, fracture model group, fracture model with mimic group, fracture model with inhibitor group （10 rats per group). The rat models of femoral shaft closed fractures were established in the other three groups except control group. BMSCs were isolated and cultured after successful modeling. After 7 days, fracture model with mimic group was transfected with miR-125b mimic. The fracture model with inhibitor group was transfected with miRNA-125b inhibitor. The morphology, expression and proliferation of BMSCs were observed. The expressions of osteogenesis gene [osteocalcin (OCN) osteopontin (OPN), Osx, Runx2 protein] and lipogenic gene [peroxisome proliferator-activated receptor γ (PPARγ)] were detected by Western blot.　Results　qPCR showed that the expression of miR-125b was up regulated in BMSCs of the fracture model [(1.48±0.18）vs (0.95±0.12)] (P<0.05). Red stained lipid droplet cells appeared in the cytoplasm of fracture model with inhibitor group at 7 days after transfection. In fracture model with mimic group, morphological characteristics of adipocytes appeared on the 14th day. Runx2, OCN and Osx in fracture model with mimic group [(0.20±0.03), (0.45±0.05), (0.34±0.04)] were lower than those in the other three groups, while PPARγ (0.51±0.05) was higher than that in the other three groups. OPN in fracture model with mimic group was lower than that in fracture model with inhibitor group [(0.52±0.06) vs (0.88±0.02)] (P < 0.05). But there was no significant difference between control group and fracture model group (P>0.05).　Conclusion　The miR-125b may negatively regulate the expression of OCN by regulating Runx2/Osx, and positively regulate the expression of PPARγ and OPN, thus regulate the osteogenesis of BMSCs.

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备注/Memo

备注/Memo:
基金项目：基金项目:上海健康医学院种子基金（HMSF-17-22-026）
更新日期/Last Update: 2019-03-20