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[1]朱名洁,李国均,贾国泉,等.苗药防感香囊对小鼠肺组织中Toll样受体7和干扰素α/β表达的影响[J].医学研究与战创伤救治(原医学研究生学报),2019,21(5):449-454.[doi:10.3969/j.issn.1672-271X.2019.05.001]
　ZHU Ming-jie,LI Guo-jun,JIA Guo-quan,et al.Effects of Miao Fanggan Sachet on the expression of TLR7, IFN- alpha/beta in lung tissue of mice[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(5):449-454.[doi:10.3969/j.issn.1672-271X.2019.05.001]
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苗药防感香囊对小鼠肺组织中Toll样受体7和干扰素α/β表达的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年5期
页码:
449-454
栏目:
基础研究
出版日期:
2019-09-06

文章信息/Info

Title:
Effects of Miao Fanggan Sachet on the expression of TLR7, IFN- alpha/beta in lung tissue of mice
文章编号:
1672-271X（2019）05-0449-06
作者:
朱名洁;  李国均;  贾国泉;  张权
作者单位：550004 贵阳，贵州医科大学感染病学教研室（朱名洁、李国均、贾国泉、张 权）
Author(s):
ZHU Ming-jie;  LI Guo-jun;  JIA Guo-quan;  ZHANG Quan
(Department of Infectious Diseases, Guizhou Medical University, Guiyang 550004, Guizhou, China)
关键词:
苗药防感香囊;  Toll样受体7;  干扰素α;  干扰素β;  非特异性免疫;  呼吸道感染
Keywords:
Miaoyao Fanggan Sachet;  Toll-like receptor 7;  interferon alpha;  interferon beta;  nonspecific immunity;  respiratory tract infection
分类号:
R511.7
DOI:
10.3969/j.issn.1672-271X.2019.05.001
文献标志码:
A
摘要:
目的 研究苗药防感香囊对小鼠肺组织Toll样受体7（TLR7）、干扰素α（IFNα）、干扰素β（IFNβ）的表达影响，从Ⅰ型干扰素信号通路角度探讨苗药防感香囊调节呼吸道免疫功能的作用机制，同时为苗药防感香囊有效预防呼吸道病毒感染提供科学理论依据。 方法 48只昆明种雄性小鼠随机分为空白对照组、香囊组、冷刺激组、香囊+冷刺激组，每组12只。香囊组、香囊+冷刺激组持续吸入苗药防感香囊30 d，第31天在已有干预基础之上给予冷刺激组、香囊+冷刺激组持续1周的冷刺激制造免疫低下模型，其后处死小鼠，留取标本检测。采用HE染色观察造模后肺组织结构，免疫组织化学染色、实时荧光PCR（Real time-PCR）、蛋白印迹法（Western blot）检测小鼠肺组织中TLR7、IFNα、IFNβ的基因/蛋白表达水平；采用酶联免疫吸附测定小鼠血清中IFNα、IFNβ分泌水平。 结果 与空白对照组比较，香囊组、香囊+冷刺激组小鼠肺组织中TLR7、IFNα/β的蛋白及mRNA表达增加（P<0.05），冷刺激组小鼠肺组织中TLR7、IFNα/β的蛋白及mRNA表达相对减少（P<0.05）；与冷刺激组比较，香囊+冷刺激组小鼠肺组织中TLR7、IFNα/β的蛋白及mRNA表达水平增加（P<0.05）；与空白对照组比较，仅香囊+冷刺激组中小鼠血清IFNα、IFNβ分泌水平升高（P<0.05），其他组间比较差异无统计学意义（P>0.05）。 结论 苗药防感香囊能够良性调节小鼠肺组织TLR7、IFNα、IFNβ基因和（或）蛋白表达水平；寒冷刺激能抑制小鼠肺组织TLR7、IFNα、IFNβ的转录与表达；苗药防感香囊能在一定程度上改善寒冷对小鼠肺组织TLR7、IFNα、IFNβ表达的抑制状态；苗药香囊可通过TLR7介导的通路中IFNα、IFNβ起到有效预防呼吸道感染，增强机体非特异免疫的作用。
Abstract:
Objective Respiratory tract infections are very common. Chinese medicine anti-allergic sachets have a good effect on adult and pediatric influenza, but the mechanism has not been widely reported. The study was aimed to investigate the effects of Miao Fanggan Sachets(MFS) on the expression of Toll-like receptor 7（TLR7）, interferon alpha（IFNα）and interferon beta（IFNβ）in lung tissues of mice. Evaluate the preventive effects of MFS inhalation on the respiratory tract of mice and to explore the underlying molecular mechanism from the perspective of type I interferon pathway. Methods Konmin male mice (n1/4 48) of SPF grade, aged from 6-7 weeks, with a body mass between 25 g and 30 g were randomly divided into 4 groups consisting of normal control group (group A),inhalation sachet group (group B), cold stimulation group (group C), and sachet plus cold stimulation group (group D).Mice in group B, D were continuously given daily 10 grams of sachets for 12 hours and the sachet was changed every 6 days. From the 31st day, cold stimulation (Continual 7 days mice were exposed in 3±1℃ ice box 15 minutes a day) was given to group C to make mice immunocompromised model. After 37 days, mice were sacrificed under anesthesia and lungs harvested lung tissues. The expression levels of TLR7, IFNα, IFNβ was assessed using immunohistochemistry, real-time polymerase chain reaction (RT-PCR) and Western blot analyses. The secretory levels of IFNα, IFNβ in serum of mice were checked by ELISA. Results Compared to the blank control group, expressions of TLR7, IFNα, IFNβ in mice lung tissues were increased in Group B and D, there was statistically difference (P<0.05).Compared to the blank cold stimulation group, expressions of TLR7, IFNα, IFNβ in lung tissues of mice were increased in Miaoyao sachet group D(P<0.05).The expression of serum IFNα and IFNβ in the sachet plus cold stimulation group were higher than those in the control group (P<0.05).There was no significant difference between the other groups. Conclusion MFS up-regulates the expressions of genes and proteins of TLR7, IFNα and IFNβ in lung tissues of mice. Cold stimulation inhibits the transcription and expression of TLR7, IFNα and IFNβ in lung tissue of mice.MFS improves the inhibition of TLR7, IFNα and IFNβ expression in lung tissue of mice in cold condition to a certain extent and enhance local non-specific immune functions as well as prevent respiratory tract infection.

参考文献/References:

1 国家卫生和计划生育委员会, 国家中医药管理局. 流行性感冒诊疗方案(2018年版)[J]. 中国感染控制杂志, 2018, 17（2）:181-184.
2 PaulesCI, SullivanSG, SubbaraoK, et al. Chasing Seasonal Influenza - The Need for a Universal Influenza Vaccine[J]. New England J Med, 2018, 378(1):7.
3 SauttoGA, KirchenbaumGA,RossTM. Towards a universal influenza vaccine:different approaches for one goal[J]. Virol J, 2018, 15(1):17.
4 SubbaraoK. Avian influenza H7N9 viruses:a rare second warning[J]. Cell Res, 2018, 28(1):1-2.
5 余江维, 王永秀, 杜江, 等. 试论贵州苗医药文化的活态传承[J]. 时珍国医国药, 2016, 25(1):1-2.
6 刘龙, 岳小强, 王丽娜, 等. 辟秽防感香囊预防流行性感冒的疗效及其免疫调节机制[J]. 中西医结合学报, 2010, 8(10):949-954.
7 孟庆志, 程明亮, 张权, 等. 苗药香囊对正常小鼠肺泡灌洗液及血清IL-2、IL-18水平的影响[J]. 中华实验和临床感染病杂志(电子版), 2012, 6(6):517-519.
8 张权,孟庆志. 苗药防感香囊对小鼠呼吸道TLR2/4、SIgA、IgG1的影响[J]. 时珍国医国药, 2012, 23(11):2905-2906, 2908.
9 金鸣昌, 晏志, 郭伟伟, 等. 苗药防感香囊预防甲型H1N1流感的应用观察[J]. 中国中医药信息杂志, 2013, 20(6):63-64.
10 王旭东, 谢飞,高益明. 辟秽香囊抗流感病毒的实验研究[J]. 中华中医药杂志, 2010, 25(6):927-929.
11 杨静, 陈涛, 祝菲, 等. 2017～2018年度中国大陆流行性感冒病例报告情况分析[J]. 热带病与寄生虫学, 2018, 16(2):63-66.
12 AkikoI,PillaiPS. Innate immunity to influenza virus infection[J]. Nat Rev Immunol, 2014, 14(5):315-328.
13 FlorianK,PeterP. Advances in the development of influenza virus vaccines[J]. Nat Rev Drug Discov, 2015, 14(3):167-182.
14 XianLI, LeiN, DuanXH, et al. A mouse model of upper respiratory tract mucosal immunity dysfunction induced by cold stimulation[J]. Chinese J Pathophysiol, 2011, 27(8):1662-1664.
15 贾国泉, 张权, 谭家武, 等. 苗药香囊对小鼠肺组织中NF-κB表达的影响[J]. 广东医学, 2017, 38(2):178-181.
16 WangH, ZhangQ, ChengML, et al. Effect of the Miaoyao Fanggan sachet-derived isorhamnetin on TLR2/4 and NKp46 expression in mice[J]. J Ethnopharmacol, 2012, 144(1):138-144.
17 ZhangQ, WangH, ChengML, et al. The miaoyao fanggan sachets regulate humoral immunity and cellular immunity in mice[J]. J Complement Integr Med, 2015, 12(1):43-51.
18 武建杰,宗海斌,路坦,等. 多聚肌苷酸多聚胞苷酸对大鼠脊髓缺血再灌注损伤后炎性反应的脊髓保护作用[J]. 医学研究生学报,2017,30(6):596-600.
19 徐迪晖,王继德,程天明,等. α干扰素对结肠癌细胞株lovo和sw1116中X染色体连锁凋亡抑制相关因子1表达调节作用[J]. 医学研究生学报,2007,20(12):1242-1244.
20 Saitoh SI, AbeF, KannoA, et al. TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells[J]. Nat Commun, 2017, 8(1):1592.
21 ZhenA, RezekV, YounC, et al. Targeting type I interferon-mediated activation restores immune function in chronic HIV infection[J]. J Clin Invest, 2017, 127(1):260-268.
22 朱芮,李敏超. 寒冷刺激支气管上皮细胞产生外泌体促肺成纤维细胞表达气道重塑相关因子[J]. 中国生物化学与分子生物学报, 2017, 33（10）:1070-1075.

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备注/Memo

备注/Memo:
收稿日期：2019-04-01
更新日期/Last Update: 2019-09-06