[1]黄婷婷,柴琳,王振杰,等.尖吻蝮蛇毒组分I对人口腔鳞状细胞癌HN4细胞凋亡及自噬的影响[J].医学研究与战创伤救治(原医学研究生学报),2020,22(01):9-14.[doi:10.3969/j.issn.1672-271X.2020.01.003]
HUANGTing-ting,CHAILin,WANGZhen-jie,et al.Effect of component I from Agkistrodon Acutus Venom on apoptosis and autophagy of human oral cancer HN4 cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(01):9-14.[doi:10.3969/j.issn.1672-271X.2020.01.003]
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尖吻蝮蛇毒组分I对人口腔鳞状细胞癌HN4细胞凋亡及自噬的影响(
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HUANGTing-ting,CHAILin,WANGZhen-jie,et al.Effect of component I from Agkistrodon Acutus Venom on apoptosis and autophagy of human oral cancer HN4 cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(01):9-14.[doi:10.3969/j.issn.1672-271X.2020.01.003]
)《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]
- 卷:
- 第22卷
- 期数:
- 2020年01期
- 页码:
- 9-14
- 栏目:
- 基础研究
- 出版日期:
- 2020-01-06
文章信息/Info
- Title:
- Effect of component I from Agkistrodon Acutus Venom on apoptosis and autophagy of human oral cancer HN4 cells
- 关键词:
- 尖吻蝮蛇毒组分I; 口腔鳞状细胞癌HN4细胞; 半胱氨酸蛋白酶-3; 凋亡; 自噬
- Keywords:
- component I from Agkistrodon Acutus Venom; oral squamous cell carcinoma HN4 cells; Caspase-3; apoptosis; autophagy
- 分类号:
- R739.85
- 文献标志码:
- A
- 摘要:
- 目的 探索尖吻蝮蛇毒组分I（AAVC-I）在人口腔鳞状细胞癌中促进细胞凋亡的作用及对自噬的影响。 方法 将所培养的人口腔鳞癌HN4细胞随机分为4组：对照组，AAVC-I低浓度组（2.5 μg/mL），AAVC-I中浓度组（5 μg/mL），AAVC-I高浓度组（10 μg/mL）。将尖吻蝮蛇毒组分I冻干粉溶于培养基中，在肿瘤细胞的对数生长期加入不同浓度AAVC-I，对照组给予相应剂量的溶剂，24 h后取标本，MTT检测肿瘤细胞的抑制率，流式细胞仪检测细胞凋亡情况，Western blot及免疫组织化学染色法检测Caspase-3蛋白的表达情况，采用JC-1试剂盒检测线粒体膜电位，免疫荧光法检测自噬微管相关蛋白轻链3(LC3)蛋白的表达。 结果 与对照组相比，给予AAVC-I低、中、高不同浓度干预后，肿瘤细胞的增殖受抑制（P<0.05），细胞凋亡率增加（P<0.01），肿瘤细胞Caspase-3表达量增加(P<0.05)，线粒体膜电位下降(P<0.05)，免疫荧光显示LC3的表达量增加(P<0.05)，并呈浓度依赖性。 结论 AAVC-I可以抑制口腔鳞状细胞癌HN4细胞的增殖，可能机制为线粒体膜电位改变及Caspase-3蛋白的表达增加有关，同时AAVC-I增加自噬活性。
- Abstract:
- Objective To explore the role of component I from Agkistrodon Acutus Venom (AAVC-I) on apoptosis and the influence on autophagy of oral squamous cell carcinoma (OSCC). Methods HN4 cells were randomly divided into four experimental groups: control group, low AAVC-I (2.5 ?g/mL), medium AAVC-I (5 ?g/mL) and high AAVC-I (10 ?g/mL). Component I of Agkistrodon Acutus Venom was dissolved in the medium. Different concentrations of AAVC-I were added to the logarithmic growth phase of tumor cells. Control group was given the corresponding dose of solvent. After 24 hours, the survival index of tumor cells was detected by MTT. Cell apoptosis was detected by flow cytometry. The expression of Caspase-3 was detected by Western blot and immunohistochemical staining. Mitochondrial membrane potential was detected by JC-1 Kit. And the expression of LC3 was detected by immunofluorescence. Results Compared with the control group, the growth and proliferation of the tumor cells and mitochondrial membrane potential were inhibited (P<0.05). Cell apoptosis rate, the expression of Caspase-3, and the expression of LC3 were increased (P<0.01). Conclusion Component I of Agkistrodon Acutus Venom can promote the apoptosis of HN4 cells, and affect autophagic activity, which may be associated with changes of mitochondrial membrane potential and the increased expression of Caspase-3.
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- 备注/Memo:
- 基金项目：皖南医学院重点科研项目培育基金（WK2015Z08）
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