|本期目录/Table of Contents|

[1]康治理,武振方,刘晓伟,等.基于生物信息学分析的hsamiR206靶基因预测[J].医学研究与战创伤救治(原医学研究生学报),2020,22(2):118-123.[doi:10.3969/j.issn.1672-271X.2020.02.002]
　KANG Zhi li,WU Zhen fang,LIU Xiao wei,et al.Prediction of hsamiR206 target gene based on bioinformatics analysis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(2):118-123.[doi:10.3969/j.issn.1672-271X.2020.02.002]
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基于生物信息学分析的hsamiR206靶基因预测()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年2期
页码:
118-123
栏目:
基础研究
出版日期:
2020-03-11

文章信息/Info

Title:
Prediction of hsamiR206 target gene based on bioinformatics analysis
作者:
康治理; 武振方; 刘晓伟; 许斌
作者单位：233030 蚌埠，蚌埠医学院研究生院（康治理）；210002 南京，东部战区总医院（原南京军区南京总医院）骨科（武振方、刘晓伟、许斌）
Author(s):
KANG Zhili1; WU Zhenfang2; LIU Xiaowei2; XU Bin2
(1. School of Graduate，Bengbu Medical College,Bengbu 233030，Anhui, China;2.Department of Orthopaedics，General Hospital of Eastern Theater Command，PLA，Nanjing 210002，Jiangsu，China）
关键词:
hsamiR206; 生物信息学; 靶基因; 信号通路
Keywords:
hsamiR206; bioinformatics; targetgene; signailing pathway
分类号:
R363；Q522
DOI:
10.3969/j.issn.1672-271X.2020.02.002
文献标志码:
A
摘要:
目的应用生物信息学方法分析hsamiR206序列，并进行靶基因功能富集分析、信号通路富集分析，靶基因编码蛋白相互作用分析，为后续研究其功能提供理论基础。方法通过 miRbase、TargetScan、DIANAmicroT、MiRDB据库、VENN在线工具、DAVID在线数据库、STRING数据库及Cytoscape软件等在线工具分析hsamiR206序列及保守性，预测其靶基因，对预测的靶基因进行功能富集分析（GO分析）、KEGG通路富集分析，并筛选出关键基因。结果hsamiR206序列在各物种间高度保守，GO分析发现hsamiR206靶基因功能主要富集在生物合成调节、有机物代谢过程调节、转录调节等生物学过程，KEGG分析表明主要参与Wnt信号通路、T细胞受体信号通路、癌症通路等。蛋白互作显示编码蛋白间存在复杂的相互作用，与hsamiR206 关系最密切的蛋白有27个，如天冬氨酸谷氨酸丙氨酸天冬氨酸盒解螺旋酶5（DDX5）、人远端上游原件结合蛋白（FUBP1）、天冬氨酸谷氨酸丙氨酸组氨酸盒解螺旋酶15(DHX15)等。结论hsamiR206可能参与肿瘤发生发展中重要的生物学过程及调控机制，为进一步实验验证提供了线索。
Abstract:
Objective The bioinformatics method was used to analyze the sequence of hsamiR206 ,the functional enrichment analysis and signal pathway enrichment analysis of the target genes, and the proteinprotein interation network was used to provide atheoretical basis for the subsequent study of its function.MethodsAnalysis of hsamiR206 sequence and conservation，predict target genes by online tools such as miRbase, TargetScan, DIANAmicroT, MiRDB database, VENN online tool, DAVID online database, STRING database and Cytoscape software , GO enrichment analysis, KEGG pathway enrichment analysis and target gene encoding protein analysis were performed, and key genes were screened.ResultsThe hsamiR206 sequence was highly conserved among different species. GO analysis revealed that hsamiR206 target gene function was mainly enriched in biological processes such as biosynthesis regulation, regulation of metabolism, and transcriptional regulation. KEGG analysis indicated that it mainly participated in Wnt Signal pathway, T cell receptor signaling pathway, cancer pathway, etc. Protein interactions show complex interactions between the encoded proteins，There are 27 proteins most closely related to hsamiR206, such as DDX5, FUBP1, DHX15, etc.ConclusionhsamiR206 may be involved in important biological processes and regulatory mechanisms in tumorigenesis and development, providing clues for further experimental validation.

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备注/Memo

备注/Memo:
基金项目：南京军区医药卫生科研基金课题（15DX019）
更新日期/Last Update: 2020-03-11