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[1]杨玉琼,孟翔峰.自酸蚀树脂粘接剂合成纳米银对唾液生物膜抗菌性的初步研究[J].医学研究与战创伤救治(原医学研究生学报),2020,22(4):346-350.[doi:10.3969/j.issn.1672-271X.2020.04.003]
　YANG Yu-qiong,MENG Xiang-feng.Antibacterial activity of self-etching adhesive resins containing silver nanoparticles synthesized in situ on human plaque microcosm biofilms[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(4):346-350.[doi:10.3969/j.issn.1672-271X.2020.04.003]
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自酸蚀树脂粘接剂合成纳米银对唾液生物膜抗菌性的初步研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年4期
页码:
346-350
栏目:
基础研究
出版日期:
2020-07-20

文章信息/Info

Title:
Antibacterial activity of self-etching adhesive resins containing silver nanoparticles synthesized in situ on human plaque microcosm biofilms
作者:
杨玉琼; 孟翔峰
作者单位：210008南京，南京大学医学院附属口腔医院(南京市口腔医院)修复科（杨玉琼、孟翔峰）
Author(s):
YANG Yu-qiong; MENG Xiang-feng
(Department of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, Jiangsu, China)
关键词:
纳米银; 自酸蚀树脂粘接剂; 生物膜; 抗菌性
Keywords:
silver nanoparticles;  self-etching adhesive resin;  antibacterial activity;  biofilms
分类号:
R78
DOI:
10.3969/j.issn.1672-271X.2020.04.003
文献标志码:
A
摘要:
目的运用原位法合成纳米银对自酸蚀树脂粘接剂进行抗菌改性。方法将2-乙基己酸银添加到甲基丙烯酸叔丁基氨基乙酯中，震荡混匀后制成8%质量分数的银源溶液，再将其分别以0%、0.1%、0.2%质量分数银（Ag）添加到两步法自酸蚀树脂粘接剂Clearfil SE Bond（CSB）的粘接剂部分及一步法自酸蚀树脂粘接剂Clearfil SE One（CSO）中，光照固化后制得圆盘状试件。以透明聚酯薄膜为空白对照组；以 0%Ag CSB、0%Ag CSO为对照组；以 0.1%Ag CSB、0.1%Ag CSO、0.2%Ag CSB、0.2%Ag CSO为含银实验组。采用透射电镜观察粘接剂中生成纳米银的形貌、粒径及分散情况；采用菌落计数及活/死菌染色来评价其对唾液生物膜的抗菌性能。结果透射电镜结果显示实验组试件中有分散均匀的纳米银颗粒生成，0.1%Ag CSB组、0.2%Ag CSB组、0.1%Ag CSO组、0.2%Ag CSO组的纳米银平均粒径分别为(10.82±3.66)nm、(8.84±3.21)nm、(21.38±5.39)nm、(17.77±4.72)nm。菌落计数换算成的抗菌率结果显示，0%Ag CSO组的抗菌率[（32.12±10.55）%]显著高于0%Ag CSB组[（14.66±5.63）%]（P<0.05），但显著低于CSB和CSO的0.1%Ag组[（56.71±7.89）%、（63.52±5.91）%]和0.2%Ag组[（77.30±4.45）%、（83.75±8.59）%]的抗菌率（P<0.05）；CSB和CSO的0.2%Ag组的抗菌率显著高于它们各自的0.1%Ag组（P<0.05）；在0.1%Ag或0.2%Ag浓度下，CSB和CSO组间的抗菌率比较差异无统计学意义（P>0.05）。活/死菌染色结果显示对照组表面有大量活菌覆盖，而实验组表面有大量死菌覆盖。结论在自酸蚀树脂粘接接剂中能够原位合成纳米银，并对唾液生物膜具备一定的抗菌性。
Abstract:
ObjectiveTo incorporate silver nanoparticles into two different types of self-etching adhesive resins with a technique of in situ synthesized silver nanoparticles, and evaluate the antibacterial activity of the new dental adhesive resins.MethodsThe sliver 2-ethylhexanoate was dissolved into 2- (tert-butylamino) ethyl methacrylate to form a silver amine solution with a mass fraction of 8%. The silver amine solution was added to the two-step self-etching adhesive resin Clearfil SE Bond (CSB) and one-step self-etching adhesive resin Clearfil SE One (CSO), respectively. After curing by light, resin disks with silver mass fractions of 0%, 0.1%and 0.2% were obtained. The transparent polyester film was used as the blank control group. 0%Ag CSB and 0%Ag CSO were used as the control group. 0.1%Ag CSB, 0.1%Ag CSO, 0.2%Ag CSB and 0.2%Ag CSO were used as the silver-containing experimental group. The morphology and particle size distribution of nano-silver was observed by transmission electron microscope (TEM). The antibacterial activity of adhesive resins against biofilms were evaluated by Colony-forming unit (CFU) counts and live/dead bacteria staining.ResultsTEM results showed that the silver-containing specimens after solidification had uniform dispersion of nano-silver particles. The average particle diameters of silver nanoparticles in the 0.1%Ag CSB group, 0.2%Ag CSB group, 0.1%Ag CSO group, and 0.2%Ag CSO group were (10.82±3.66)nm, (8.84±3.21)nm, (21.38±5.39) nm, (17.77±4.72)nm, respectively. The antibacterial rate converted from CFU counts was displayed as follows: The antibacterial rate of 0% Ag CSO Group [(32.12±10.55)%] was significantly higher than that of 0% Ag CSB Group [(14.66±5.63)%] (P<0.05), but significantly lower than that of 0.1% Ag Group [(56.71±7.89)%, (63.52±5.91)%] and 0.2% Ag Group [(77.30±4.45)%, (83.75±8.59)%] (P<0.05). The antibacterial rate of 0.2% Ag group of CSB and CSO was significantly higher than that of their respective 0.1% Ag group (P<0.05). For the concentration of 0.1% Ag or 0.2% Ag, there was no significant difference between the CSB and CSO groups (P>0.05). Live/dead bacteria staining results showed that the surface of control group was covered with a large number of live bacteria, while the experimental groups were covered with a large number of dead bacteria.ConclusionThe application of in situ synthesized silver nanoparticles to self-etching adhesive resins could obtain antibacterial activity against biofilms in saliva.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金(81470781)；江苏省卫生健康委科研项目（LGY2018003）
更新日期/Last Update: 2020-07-13