[1]洪勇,周民伟,徐化交.罗哌卡因经原癌基因信号通路抑制胃癌细胞增殖和迁移的机制[J].医学研究与战创伤救治(原医学研究生学报),2020,22(5):456-460.[doi:10.3969/j.issn.1672-271X.2020.05.002]
　HONG Yong,ZHOU Min-wei,XU Hua-jiao.The mechanism of ropivacaine inhibiting the proliferation and migration of gastric cancer cells via c-MYC signaling pathway[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(5):456-460.[doi:10.3969/j.issn.1672-271X.2020.05.002]
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罗哌卡因经原癌基因信号通路抑制胃癌细胞增殖和迁移的机制()

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年5期
页码:
456-460
栏目:
基础研究
出版日期:
2020-09-09

文章信息/Info

Title:
The mechanism of ropivacaine inhibiting the proliferation and migration of gastric cancer cells via c-MYC signaling pathway
作者:
洪勇; 周民伟; 徐化交
作者单位：421002衡阳，解放军联勤保障部队第九二二医院麻醉科（洪勇、周民伟、徐化交）
Author(s):
HONG Yong; ZHOU Min-wei; XU Hua-jiao
(Department of Anesthesiology，the 922th Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army，Hengyang 421002,Hunan,China)
关键词:
罗哌卡因; c-MYC信号通路; 胃癌; 细胞增殖; 细胞迁移
Keywords:
ropivacaine;  c-MYC signaling pathway;  gastric cancer;  cell proliferation;  cell migration
分类号:
R735.2
DOI:
10.3969/j.issn.1672-271X.2020.05.002
文献标志码:
A
摘要:
目的探讨罗哌卡因经原癌基因(c-MYC)信号通路抑制胃癌细胞增殖和迁移的机制。方法体外培养人胃癌MGC-803细胞进行实验，分为对照组、罗哌卡因低剂量组、罗哌卡因中剂量组、罗哌卡因高剂量组和顺铂组。罗哌卡因低、中、高剂量组分别给予终浓度为100、200、400 μg/mL的罗哌卡因；顺铂组给予终浓度为10 mmol/mL的顺铂；对照组无处理。继续培养24 h后，细胞计数试剂盒8(CCK-8)法检测细胞增殖率，细胞划痕法检测MGC-803细胞迁移能力，同时检测MGC-803细胞中蛋白激酶C（PKC）、c-MYC、金属蛋白酶-9（MMP-9）、磷脂酰肌醇3激酶（PI3K）、蛋白激酶B（AKT）和哺乳动物雷帕霉素靶蛋白（mTOR）蛋白表达。结果罗哌卡因低、中、高剂量组和顺铂组的MGC-803细胞增殖率[（92.05±8.24）%、（76.26±6.93）%、（53.18±5.86）%、（42.18±15.17）%]和迁移能力[（36.34±4.03）μm、（31.21±3.56）μm、（24.60±2.57）μm、（18.07±1.26）μm]较对照组[（100.00±5.91）%、（40.12±5.17）μm]明显降低（P＜0.05）；上述各组MGC-803细胞中PKC[（0.62±0.06）、（0.50±0.08）、（0.42±0.08）、（0.36±0.10）]、c-MYC[（0.53±0.09）、（0.49±0.15）、（0.43±0.02）、（0.31±0.04）]、MMP-9[（0.47±0.03）、（0.42±0.05）、（0.30±0.04）、（0.21±0.06）]、PI3K[（0.39±0.04）、（0.33±0.08）、（0.28±0.04）、（0.25±0.01）]、AKT[（0.50±0.10）、（0.43±0.09）、（0.24±0.02）、（0.16±0.03）]和mTOR[（0.42±0.05）、（0.36±0.03）、（0.30±0.02）、（0.25±0.04）] 表达较对照组[（0.74±0.08）、（0.63±0.06）、（0.53±0.05）、（0.46±0.07）、（0.71±0.04）、（0.49±0.06）]明显降低（P＜0.05），且各罗哌卡因剂量组降低强度呈剂量依赖性，但各指标均仍高于顺铂组（P＜0.05）。结论罗哌卡因可抑制人胃癌MGC-803细胞增殖和迁移，其机制可能与抑制c-MYC信号通路的激活有关。
Abstract:
ObjectiveTo investigate the mechanism of ropivacaine inhibiting the proliferation and migration of gastric cancer cells via Proto-oncogene(c-MYC) signaling pathway.MethodsMGC-803 cells of human gastric cancer were cultured in vitro，and the control group was not treated. The final concentration of ropivacaine was 100，200，400 μg/mL in the low-dose ropivacaine group，the middle dose ropivacaine group and the high-dose ropivacaine group respectively. The final concentration of cisplatin was 10 mmol/mL in the cisplatin group. After continuous culture for 24 hours，the cell proliferation rate was detected by Cell count kit 8 (CCK-8)method，the migration ability of MGC-803 cells was detected by cell scratch method，and the expressions of Protein kinase C(PKC)，c-MYC，Matrix metalloproteinase-9(MMP-9)，Phosphatidylinositol 3 kinase(PI3K)，Protein kinase B(Akt) and mammalian Target of rapamycin(mTOR) protein in MGC-803 cells were detected.ResultsThe proliferation rate of MGC-803 cells in each ropivacaine dose group and cisplatin group [（92.05±8.24）%，（76.26±6.93）%，（53.18±5.86）%，（42.18±15.17）%] and migration ability [（36.34±4.03）μm，（31.21±3.56）μm，（24.60±2.57）μm，（18.07±1.26）μm] were significantly lower than those in the control group [（100.00±5.91）%，（40.12±5.17）μm] (P＜0.05).The expressions of PKC [(0.62±0.06)，(0.50±0.08)，(0.42±0.08)，(0.36±0.10)], c-MYC [(0.53±0.09)，(0.49±0.15)，(0.43±0.02)，(0.31±0.04)]，MMP-9 [(0.47±0.03)，(0.42±0.05)，(0.30±0.04)，(0.21±0.06)]，PI3K [ (0.39±0.04)，(0.33±0.08)，(0.28±0.04)，(0.25±0.01]，AKT [(0.50±0.10)，(0.43±0.09)，(0.24±0.02) ，(0.16±0.03)] and mTOR [(0.42±0.05)，(0.36±0.03)，(0.30±0.02)，(0.25±0.04)] in MGC-803 cells were significantly lower than those in the control group [（0.74±0.08）,（0.63±0.06）,（0.53±0.05）,（0.46±0.07）,（0.71±0.04）,（0.49±0.06）] (P<0.05) ，and the reduction intensity of each ropivacaine dose group was dose-dependent，but the levels of each indicators were still higher than those in the cisplatin group(P<0.05).ConclusionRopivacaine can inhibit the proliferation and migration of human gastric cancer MGC-803 cells，and its mechanism may be related to the inhibition of the activation of c-MYC signaling pathway.

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备注/Memo:
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更新日期/Last Update: 2020-09-09