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[1]张海鹏,徐琳,宋彦霖,等.有氧跑台训练对小鼠左右心肌特定miRNAs的影响[J].医学研究与战创伤救治(原医学研究生学报),2021,23(03):235-239.[doi:10.3969/j.issn.1672-271X.2021.03.003]
　ZHANG Hai-peng,XU Lin,SONG Yan-lin,et al.Effects of 8-week aerobic treadmill training on specific miRNAs of left and right myocardium in mice[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2021,23(03):235-239.[doi:10.3969/j.issn.1672-271X.2021.03.003]
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有氧跑台训练对小鼠左右心肌特定miRNAs的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第23卷
期数:
2021年03期
页码:
235-239
栏目:
出版日期:
2021-06-20

文章信息/Info

Title:
Effects of 8-week aerobic treadmill training on specific miRNAs of left and right myocardium in mice
作者:
张海鹏; 徐琳; 宋彦霖; 徐盛嘉; 杨靖; 马继政; 钟勇
作者单位：210014南京，南京体育学院运动健康学院（张海鹏、徐琳、宋彦霖）；211101南京，解放军陆军工程大学军事运动科学研究中心（徐盛嘉、杨靖、马继政）；210002南京，东部战区总医院（原南京军区南京总医院）健康医学科（钟勇）
Author(s):
ZHANG Hai-peng; XU Lin ; SONG Yan-lin; XU Sheng-jia; YANG Jing; MA Ji-zheng; ZHONG Yong
（1.Department of Exercise and Heath, Nanjing Sport Institute, Nanjing 210014, Jiangsu，China; 2. The Research Center of Military Exercise Science, the Army Engineering University of PLA, Nanjing 211101, Jiangsu，China；3.Department of Health Medicine, General Hospital of Eastern Theater Command，PLA，Nangjing 210002，Jiangsu，China）
关键词:
miRNAs; 心肌; 有氧训练; 心脏肥大
Keywords:
microRNAs; myocardium; aerobic training;  cardiac hypertrophy
分类号:
G804.5
DOI:
10.3969/j.issn.1672-271X.2021.03.003
文献标志码:
A
摘要:
目的有氧训练可导致独特的心脏保护表型，但左右心肌适应存在不同。文章评定8周有氧训练对小鼠左右心肌特定miRNAs的差异表达，从miRNAs参与心肌有氧运动适应的视角揭示8周有氧跑台训练对特定miRNAs表达水平的影响，为运动训练提供参考依据。方法8周龄C57BL/6J雄性小鼠48只，按体重随机数字分组划分对照组和训练组，每组24只。2组在第0周进行跑台预适应及递增负荷测试后，运动组进行8周有氧跑台训练，对照组正常饲养不进行跑台训练。记录2组小鼠0~8周体重变化及第0、4、8周力竭速度的变化，在第8周递增负荷测试结束48h后取左右心肌，利用实时荧光定量PCR（RT-qPCR）检测肌组织特异miRNAs及其他miRNAs的变化情况。结果8周训练后，训练组体重显著下降（P＜0.05），递增负荷测试成绩明显上升（P＜0.05）；与对照组比较，训练组左心室心肌特异miR-208b和miR-499的水平显著减小（P<0.01；P<0.05），分别为对照组的1.96倍及2.59倍；但在右心室心肌中差异无统计学意义（P>0.05）。miR-208a在小鼠左右心室的心肌中并无显著性改变（P>0.05）；与对照组比较，训练组左心室心肌中的miR-21与miR-17-3p水平显著增加（P<0.05；P<0.05），分别为对照组的2.53倍及1.13倍；但miR-21与miR-17-3p在右心室心肌中的水平差异无统计学意义（P>0.05）。有氧训练不影响这些miRNAs在右心室心肌中的表达。结论8周有氧运动后心肌特定miRNAs在左右心室中表达水平并不一致，结构相关的miRNAs可能参与调控左室心肌肥大。
Abstract:
ObjectiveAerobic training can lead to a unique cardiac protective phenotype, but there are differences in the adaptation of the left and right myocardium. In this study, the effects of 8 weeks of aerobic training on specific miRNAs in the left and right myocardium of mice were evaluated to provide a reference for exercise training and assessed the differential expression of the effects of specific miRNAs in myocardium.MethodsA total of 48 8-week-old C57BL/6J male mice were randomly divided into con-trol group and training group according to body weight. Changes in body weight and exhaustion rate at week 0, 4 and 8 were recorded. Left and right myocardium were collected 48 hours after the incremental load test at week 8, using real-time fluorescent quantitative PCR (RT-qPCR) to detect myocardial certain specific miRNAs and other changes of the miRNAs. After the two groups underwent treadmill pre-adaptation and incremental load tests in week 0, the exercise group received aerobic treadmill training for 8 weeks, while the control group was given normal diet without treadmill training.ResultsAfter 8 weeks of training, the NT group’s body weight was significantly decreased (P<0.05). The score of incremental load test was significantly increased (P<0.05). Compared with the CON, the left ventricular myocardial specific miR-208b and miR-499 levels in NT were 1.96 times and 2.59 times more than that in the control group, respectively (P<0.01; P<0.05). But there was no significant difference in the right ventricular myocardium (P>0.05). miR-208a was not significantly changed in the myocardium of the left and right ventricles of mice (P>0.05). The levels of miR-21 and miR-17-3p in the left ventricular myocardium of the NT were 2.53 times and 1.13 times more than that of the control group (P<0.05; P<0.05). But there was no significant difference in the levels of miR-21 and miR-17-3p in the right ventricular myocardium (P>0.05). Aerobic training did not affect the expression of these miRNAs in the right ventricular myocardium.ConclusionAfter 8 weeks of aerobic exercise, the expression levels of specific miRNAs in myocardium were not consistent in the left and right ventricles, and structure-related miRNAs may be involved in regulating left ventricular myocardial hypertrophy.

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备注/Memo

备注/Memo:
基金项目：全军军事类研究生资助课题（KYJXJQJY2001）；江苏省研究生科研与实践创新计划课题（KYCX20_1729）；军委后勤保障部卫生局保健专项课题（18BJZ13）
更新日期/Last Update: 2021-06-17