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[1]谢晖,周万青,沈瀚.鹑鸡肠球菌对万古霉素耐药性监测及耐药基因研究[J].医学研究与战创伤救治(原医学研究生学报),2021,23(03):249-252.[doi:10.3969/j.issn.1672-271X.2021.03.006]
　XIE Hui,ZHOU Wan-qing,SHEN Han.Surveillance of vancomycin resistance and studies on resistance genes of Enterococcus gallinarum[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2021,23(03):249-252.[doi:10.3969/j.issn.1672-271X.2021.03.006]
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鹑鸡肠球菌对万古霉素耐药性监测及耐药基因研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第23卷
期数:
2021年03期
页码:
249-252
栏目:
基础研究
出版日期:
2021-06-20

文章信息/Info

Title:
Surveillance of vancomycin resistance and studies on resistance genes of Enterococcus gallinarum
作者:
谢晖; 周万青; 沈瀚
作者单位：210008南京，南京大学医学院附属鼓楼医院检验科（谢晖、周万青、沈瀚）
Author(s):
XIE Hui;  ZHOU Wan-qing;  SHEN Han
（Department of Laboratory Medicine，Nanjing Drum Tower Hospital，the Affiliated Hospital of Nanjing University Medical School，Nanjing 210008，Jiangsu，China）
关键词:
鹑鸡肠球菌; 万古霉素耐药; vanA基因; 全基因组测序
Keywords:
Enterococcus gallinarum;  vancomycin resistance;  vanA gene;  whole genome sequencing
分类号:
R446.5
DOI:
10.3969/j.issn.1672-271X.2021.03.006
文献标志码:
A
摘要:
目的调查临床分离鹑鸡肠球菌对万古霉素的耐药情况及耐药机制。方法采用VITEK 2 系统GP67卡对2019年1-12月南京大学医学院附属鼓楼医院临床分离15株鹑鸡肠球菌进行药敏试验，采用E-test法复核菌株对万古霉素最低抑菌浓度（MIC）；采用PCR及测序技术分析万古霉素耐药决定基因vanA、vanB、vanC1及vanC2/3；采用Illumina HiSeq 2000 测序技术对菌株基因组DNA进行双端测序（PE），利用生物信息学对菌株全基因组序列分析及耐药基因、毒力基因分析。结果15株鹑鸡肠球菌对万古霉素的MIC值集中在4 mg/L和8 mg/L，所占比例分别为40%和33.3%，检出1株（6%）万古霉素高水平耐药株EG17906（MIC为256 mg/L），该菌株除对万古霉素和替考拉宁耐药外，对其余常用抗生素均敏感；15株菌株均检出vanC1基因，EG17906菌株同时检出vanA基因；EG17906菌株经全基因组测序分析，基因组大小为3765 197 bp，鸟嘌呤（G）和胞嘧啶（C）所占的比率GC含量为40.4%，总基因数为3754，基因组含有3592个编码序列，60个tRNA编码基因以及10个完整的rRNA基因编码操纵子。耐药基因预测分析该菌株携带VanC1XY、VanHAX、erm(A)、erm(A)、tet(O)、cat(pC221)。全基因序列提交至美国国家生物技术信息中心（NCBI）数据库，GeneBank号为JABMDB000000000。结论鹑鸡肠球菌携带vanC1对万古霉素呈低水平耐药，而同时携带vanA基因可造成菌株高水平耐药，需引起重视。
Abstract:
ObjectiveTo investigate clinical isolation of Enterococcus gallinarum against vancomycin and its resistance mechanism.Methods15 strains of Enterococcus gallinarum isolated from Nanjing Gulou Hospital from January to December 2019 were tested for drug sensitivity using VITEK 2 system GP67, and the minimum inhibitory concentration (MIC) against vancomycin was verified by e-test. PCR and sequencing techniques were utilized to analyze vancomycin resistance gene vanA, vanC1 and vanC2/3. Illumina HiSeq 2000 sequencing technologies were used for genomic DNA sequencing of the bacterial strain and Paired end (PE) sequencing was performed. Bioinformatics was used only for genomic sequencing of the bacterial strain and analysis of drug-resistant genes and virulence genes.ResultsThe MIC values of 15 strains of Enterococcus gallinarum against vancomycin were concentrated in 4 mg/L and 6 mg/L, accounting for 40% and 33.3%, respectively. Among them, 1 strain (6%) with high vancomycin resistance EG17906 (MIC was 256 mg/L) was detected, which was sensitive to other common antibiotics except vancomycin and teicoplanin . The gene of vanC1 was detected in all the 15 strains, while vanA gene was detected in the EG17906 strain. The whole genome sequencing analysis of EG17906 strain showed that the genome size was 3765197 bp, GC content was 40.4%, and the total number of genes was 3754. The genome contained 3592 coding sequences, 60 tRNA coding genes and 10 complete rRNA coding operons.Drug resistance gene prediction analysis the strain carries VanC1XY, VanHAX, erm(A)，erm(A) ，tet(O)，cat(pC221).The complete gene sequence is submitted to the NCBI database, GeneBank is No. JABMDB000000000.ConclusionEnterococcus gallinarum with low level of vancomycin resistance, while vanA gene can cause a high level of drug resistance, which should be paid attention.

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备注/Memo

备注/Memo:
基金项目：南京市医学科技发展项目（YKK17056）
更新日期/Last Update: 2021-06-17