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[1]闵逸飞,应小燕.miR-205-5p负靶向调控血管内皮生长因子A对子宫内膜异位症的影响[J].医学研究与战创伤救治(原医学研究生学报),2022,24(6):561-568.[doi:10.3969/j.issn.1672-271X.2022.06.001]
　MIN Yi-fei,YING Xiao-yan.MiR-205-5p affects the development of human endometriosis by negatively targeting vascular endothelial growth factor A[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2022,24(6):561-568.[doi:10.3969/j.issn.1672-271X.2022.06.001]
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miR-205-5p负靶向调控血管内皮生长因子A对子宫内膜异位症的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第24卷
期数:
2022年6期
页码:
561-568
栏目:
基础研究
出版日期:
2023-01-18

文章信息/Info

Title:
MiR-205-5p affects the development of human endometriosis by negatively targeting vascular endothelial growth factor A
作者:
闵逸飞; 应小燕
作者单位：213000常州，南京医科大学附属常州第二人民医院妇科（闵逸飞）；210000南京，南京医科大学第二附属医院妇产科（应小燕）
Author(s):
MIN Yi-fei1; YING Xiao-yan2
（1.Department of Gynecology, the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou 213000，Jiangsu, China；2.Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210000，Jiangsu, China）
关键词:
子宫内膜异位症; miR-205-5p; 血管内皮生长因子A; 子宫内膜基质细胞
Keywords:
endometriosis;  miR-205-5p;  vascular endothelial growth factor A;  endometrial stromal cells
分类号:
R711.71
DOI:
10.3969/j.issn.1672-271X.2022.06.001
文献标志码:
A
摘要:
目的研究miR-205-5p和血管内皮生长因子A（VEGFA）在子宫内膜异位中的作用机制。方法采用RT-PCR技术检测子宫异位内膜组织中miR-205-5p的表达。利用CCK8、EdU和流式细胞仪分别检测子宫内膜基质细胞的活力、增殖和凋亡。利用Western blot实验检测增殖相关蛋白和凋亡相关蛋白的表达。利用划痕实验和Transwell检测miR-205-5p过表达对子宫内膜基质细胞迁移和侵袭的影响。利用生物信息学预测miR-205-5p与VEGFA的结合位点，并利用双荧光素酶报告、RT-PCR和Western blot实验验证VEGFA是miR-205-5p的靶标。利用拯救实验验证miR-205-5p通过负调控VEGFA在子宫内膜异位症中发挥作用。结果子宫内膜异位症患者的组织和血液中miR-205-5p的表达显著降低（P<0.01）。过表达miR-205-5p后，可显著抑制子宫内膜基质细胞的增殖（P<0.01），迁移与侵袭（P<0.01），并促进凋亡（P<0.01）。miR-205-5p负靶向调控VEGFA。与miR-205-5p模拟+质粒组相比，miR-205-5p模拟+VEGFA组的细胞活力和细胞增殖显著升高（P<0.01），细胞的凋亡显著降低（P<0.01），细胞的迁移和侵袭能力显著升高（P<0.01）。结论miR-205-5p通过负靶向调控VEGFA影响子宫内膜异位症的发展。
Abstract:
ObjectiveThis study aims to figure out the mechanism of miR-205-5p and vascular endothelial growth factor A (VEGFA) in endometriosis.MethodsThe expression of miR-205-5p in ectopic endometrial tissues was detected by RT-PCR. Viability, proliferation, and apoptosis of the endometrial stromal cells were measured using CCK8 assay, EdU assay, and flow cytometry, respectively. The expressions of proliferation related and apoptosis related proteins were detected using Western blot assay. Scratch assay and Transwell assay were utilized to detect the effect of miR-205-5p overexpression on endometrial stromal cell migration and invasion. Bioinformatics was used to predict the binding sites of miR-205-5p and VEGFA, and dual luciferase reporter, RT-PCR and Western blot experiments were used to validate that VEGFA was a target of miR-205-5p. Rescue experiments were utilized to verify that miR-205-5p played a role in endometriosis by negatively regulating VEGFA.ResultsMiR-205-5p expression was significantly reduced in the tissues and blood of women with endometriosis (P<0.01). Overexpression of miR-205-5p significantly inhibited endometrial stromal cell proliferation (P<0.01), migration and invasion (P<0.01), and promoted apoptosis (P<0.01). MiR-205-5p negatively targeted regulation of VEGFA. Compared with the miR-205-5p mimic + vector group, the miR-205-5p mimic + VEGFA group exhibited significantly higher cell viability and cell proliferation (P<0.01), significantly lower cell apoptosis (P<0.01), and significantly lower cell migration and invasion abilities (P<0.01).ConclusionmiR-205-5p negatively regulates VEGFA and affects the development of the endometriosis.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2023-01-18