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[1]李周兰,洪新如,何春妮.盆底器官脱垂患者阴道前壁miR-29a/b和转化生长因子β1及胶原蛋白含量的变化[J].医学研究与战创伤救治(原医学研究生学报),2019,21(01):49-52.[doi:10.3969/j.issn.1672-271X.2019.01.011]
　LI Zhou-lan,HONG Xin-ru,HE Chun-ni.Expression levels of miR-29a/b collagen and TGF-β1 in vaginal tissues of patients with pelvic organ prolapse[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(01):49-52.[doi:10.3969/j.issn.1672-271X.2019.01.011]
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盆底器官脱垂患者阴道前壁miR-29a/b和转化生长因子β1及胶原蛋白含量的变化()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年01期
页码:
49-52
栏目:
临床研究
出版日期:
2019-01-20

文章信息/Info

Title:
Expression levels of miR-29a/b collagen and TGF-β1 in vaginal tissues of patients with pelvic organ prolapse
作者:
李周兰; 洪新如; 何春妮
作者单位：350025福州,福建医科大学福总临床医学院(第九○○医院)妇产科（李周兰）；350025福州，解放军联勤保障部队第九○○医院（原南京军区福州总医院）妇产科（洪新如、何春妮）
Author(s):
LI Zhou-lan1;  HONG Xin-ru2;  HE Chun-ni2
(1.Department of Obstetrics and Gynecology, Fujian Medical University (the 900th Hospital), Fuzhou 350025,Fujian,China;2.Department of Obstetrics and Gynecology，the 900th Hospital of the Joint Logistics Support Force，PLA, Fuzhou 350025,Fujian,China)
关键词:
盆底器官脱垂; 微小RNA-29a/b; 胶原蛋白; 转化生长因子-β1
Keywords:
pelvic organ prolapse; microRNA-29a/b; collagen; transforming growth factor beta 1
分类号:
R711.23
DOI:
10.3969/j.issn.1672-271X.2019.01.011
文献标志码:
A
摘要:
目的 观察盆底器官脱垂（POP）患者阴道组织中微小RNA-29a/b(miR-29a/b)、转化生长因子-β1(TGF-β1)及胶原蛋白含量的变化，探讨POP发病的可能机制。方法 收集解放军联勤保障部队第九○○医院妇科2016年1-12月期间有良性适应证并行子宫切除术的Ⅲ、Ⅳ期POP患者（脱垂组）和非POP患者（对照组）各20例，术中取阴道前壁组织，RT-PCR检测miR-29a/b的表达水平，免疫印迹方法 检测Ⅰ、Ⅲ型胶原蛋白及TGF-β1的含量。结果 脱垂组miR-29a/b表达水平显著高于对照组（7.36∶1，P=0.008）；脱垂组Ⅲ型胶原蛋白含量显著低于对照组[（0.73±0.17） vs （1.11±0.27），P=0.000]；脱垂组Ⅰ型胶原蛋白含量与对照组比较差异无统计学意义[（0.78±0.79） vs （1.32±1.76），P=0.116]；脱垂组TGF-β1显著高于对照组[（2.72±3.12） vs （1.73±2.33），P=0.020]。结论 POP患者miR-29a/b表达和TGF-β1的含量增加、胶原蛋白减少，可能是盆底器官脱垂发生发展的机制之一。
Abstract:
Objective To investigate the expression profile ofmicroRNA-29, collagen and transforming growth factor-β1 (TGF-β1) in vaginal tissues of patients with pelvic organ prolapse (POP) and the controls.Methods Twenty patients with POP and 20 patients without POPwere recruited in this study. Total mRNAs of miR-29a/b were detected by real-time RT-PCR and expression levels of collagen Ⅰ, Ⅲ and TGF-β1 were quantified by Western blotting.Results The expression of miR-29a/b in POP group was significantly higher than that in non-POP group（7.36∶1, P=0.008). The expression of Collagen III was significantly lower in POP group than that in the control group ( 0.73±0.17 vs 1.11±0.27, P=0.00). And the expression of Collagen I in POP group was lower than that in control group, but there was no significant difference between the two groups (0.78±0.79 vs 1.32±1.76, P=0.116). The expression of TGF- β1 in POP group was significantly higher than that in the control group ( 2.72±3.12 vs 1.73±2.33,P=0.020).Conclusion The expression of miR-29a/b and TGF-β1 in POP patients was increased, which resulted in the decrease of collagen protein in POP′s pelvic floor tissue through a series of regulatory approaches. This may be one of the mechanisms of POP.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：福建省自然科学基金（2014J01428）
更新日期/Last Update: 2019-01-20