|本期目录/Table of Contents|

[1]费燕,林秀丽,胡永狮,等.柱前衍生-HPLC法测定人血清中丙戊酸钠的方法改进[J].医学研究与战创伤救治(原医学研究生学报),2010,12(05):418.
　FEI Yan,LIN Xiu-li,HU Yong-shi,et al.Improvement of precolumn derivatization HPLC assay method in determination of sodium valproate in human serum[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(05):418.
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柱前衍生-HPLC法测定人血清中丙戊酸钠的方法改进()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年05期
页码:
418
栏目:
出版日期:
2010-09-20

文章信息/Info

Title:
Improvement of precolumn derivatization HPLC assay method in determination of sodium valproate in human serum
文章编号:
1672-271X(2010)05-0418-03
作者:
费燕; 林秀丽; 胡永狮; 张荣
363000 福建漳州，解放军175医院药剂科
Author(s):
FEI Yan;  LIN Xiu-li;  HU Yong-shi;  ZHANG Rong .
Department of Pharmacy, 175 Hospital of PLA / the Affiliated Southeast Hospital of Xiamen University, Zhangzhou, Fujian 363000, China
关键词:
高效液相色谱法; 丙戊酸钠; 柱前衍生
Keywords:
HPLC;  sodium valproate;  precolumn derivatization
分类号:
R971 +.16；R917
DOI:
-
文献标志码:
A
摘要:
目的 建立快速柱前衍生-HPLC法测定人血清中丙戊酸钠(VPA)。方法 选择对溴苯甲酰甲基溴为衍生化试剂，壬酸为内标，Hypersil BDS C18 (4.6 mm × 200 mm，5 μm)为分析柱；流动相为乙腈-水(75∶25，V/V)；检测波长254 nm；流速1.0 ml/min；柱温40 ℃。结果 VPA的血清浓度在25.0～250.0 mg/L范围内线性关系良好(r = 0.9991)，平均回收率为(100.43±0.73)%，日内、日间RSD均< 5%；123例服用 VPA 的癫痫患者应用本法监测，有53.7 %的患者血药浓度在有效浓度范围之内，46.3 %的患者血药浓度低于或高于有效浓度。结论 本法快速、简便、准确，适合临床常规监测 VPA 血清浓度需要。
Abstract:
Objective To establish a rapid analysis method for the determination of sodium valproate in serum by HPLC after precolumn derivatization.Methods p-bromophenacyl bromide was selected as derivative reagent.Nonanoic acid was used as an internal standard.The analytical column was Hypersil BDS C18 (4.6 mm×200 mm,5 μm).The mobile phase consisted of acetonitrile-water(75∶25,V/V).The detection wavelength was 254 nm and the flow rate was 1.0 ml/min, column temperature was 40℃.Results The linear calibration curve was obtained in the concentration range of 25.0~250.0 mg/L (r=0.9991).The mean relative recovery was (100.43±0.73)%.The relative standard deviation (RSD) of within day and between day were all less than 5%.The method was used for monitoring serum sodium valproate concentration of 123 epileptic patients, 53.7 % of the patients who had taken sodium valproate was found the serum concentration in effective level and 46.3 % lower or higher than effective level.Conclusion This method is found to be rapid, accurate, sensitive and more suitable for the determination of the concentration of sodium valproate in serum.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-09-20