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[1]王 培,管云枫,徐添颖,等.G-CSF对大鼠烧伤合并海水浸泡后内皮祖细胞的影响[J].医学研究与战创伤救治(原医学研究生学报),2013,15(01):1-3.
　WANG Pei,GUAN Yun-feng,XU Tian-ying,et al.Effects of granulocyte colony-stimulating factor on the number of circulating endothelial progenitor cells in burned rats with seawater immersion[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(01):1-3.
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G-CSF对大鼠烧伤合并海水浸泡后内皮祖细胞的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第15卷
期数:
2013年01期
页码:
1-3
栏目:
出版日期:
2013-01-20

文章信息/Info

Title:
Effects of granulocyte colony-stimulating factor on the number of circulating endothelial progenitor cells in burned rats with seawater immersion
文章编号:
1672-271X(2013)01-0001-03
作者:
王 培; 管云枫; 徐添颖; 缪朝玉
200433 上海，第二军医大学药学院药理学教研室
Author(s):
WANG Pei; GUAN Yun-feng; XU Tian-ying; MIAO Chao-yu.
Department of Pharmacology,Second Military Medical University,Shanghai 200433,China
关键词:
烧伤; 海水浸泡; 粒细胞集落刺激因子; 内皮祖细胞
Keywords:
burn;  seawater immersion;  granulocyte colony-stimulating factor;  endothelial progenitor cell
分类号:
R826.54
DOI:
-
文献标志码:
A
摘要:
目的 观察早期应用粒细胞集落刺激因子(G-CSF)对大鼠烧伤合并海水浸泡后血液中的内皮祖细胞(endothelial progenitor cells，EPC)的影响。方法 将26只SD大鼠随机分成3组:对照组、烧伤合并海水浸泡模型组和烧伤合并海水浸泡+ G-CSF实验组。在模型制备后的30 min、2 h、6 h和24 h，使用双色荧光标记流式细胞术检测不同组别大鼠的血中EPC数量。结果 烧伤合并海水浸泡使血中EPC的数量显著降低，G-CSF可以部分逆转烧伤合并海水浸泡对EPC数量减少的作用。结论 在烧伤合并海水浸泡情况下，使用G-CSF可增加血中EPC数量，并可能增强创面的修复能力。
Abstract:
Objective To study the influence of application of granulocyte colony-stimulating factor (G-CSF)on the number of circulating endothelial progenitor cells (EPC)in burned rats with seawater immersion.Methods Rats were randomly divided into three groups:normal control group,burns + seawater immersion model group and burns + seawater immersion + G-CSF group.The blood was harvested and the peripheral mononuclear blood cells were isolated by density gradient centrifugation using Ficol.The number of EPCs was determined using flow cytometry with FITC-Sca-1 and PE-VEGFR2 at four time points (30 minutes,2 hours,6 hours and 24 hours).Results Burn plus seawater immersion could significantly reduce the number of EPC in the blood.In contrast,G-CSF partly reversed this phenotype.Conclusion The G-CSF may be a promising agent in treating burn and seawater immersion.

参考文献/References:

[1]Urbich C,Dimmeler S.Endothelial progenitor cells[J].Circulation Research,2004,95(4):343-353.
[2]Wang P,Xu TY,Guan YF,et al.Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway[J].Ann Neurol,2011,69(2):360-374.
[3]阮 兢,郑庆亦,陈锦河,等.成批烧伤的卫勤组织与临床救治[J].东南国防医药,2011,13(1):5-7.
[4]李 勇,李国华,李明华.海水浸泡伤早期救治原则[J].临床军医杂志,2008,12(6):12-17.
[5]李卫东,宋 斌.海上联合作战卫勤保障的主要特点与对策[J].海军医学杂志,2007,28(3):230-232.
[6]宗 林,倪洋林,王玉秀,等.光棒及纤维支气管镜在头面部烧伤插管中的应用[J].东南国防医药,2011,13(5):393-395.
[7]An J,Li A,Yang Z,et al.Dynamic changes of fibrinopeptides A and B in sera of burn patients and their effects on vascular endothelial cells[J].Burns,1994,20(2):128-131.
[8]Infanger M,Schmidt O,Kossmehl P,et al.Vascular endothelial growth factor serum level is strongly enhanced after burn injury and correlated with local and general tissue edema[J].Burns,2004,30(4):305-311.
[9]晏 泽.烧伤后一氧化氮,内皮素动态变化与烧伤的关系[J].医学综述,1999,5(4):180-183.
[10]闫 红,赖西南,葛衡江.两种复合战伤合并海水浸泡对血管内皮细胞损伤的比较研究[J].中华航海医学与高气压医学杂志,2003,10(3):133-135.
[11]闫 红,赖西南,葛衡江.战伤合并海水浸泡对内皮细胞凝血功能的影响[J].中华航海医学与高气压医学杂志,2006,13(2):121-123.
[12]马 军,王甲汉,李志清,等.大鼠烫伤合并海水浸泡后创面病理学改变的特点[J].南方医科大学学报,2009,29(6):1297-1299.
[13]Urbich C,Dimmeler S.Endothelial progenitor cells:characterization and role in vascular biology[J].Circ Res,2004,95(4):343-353.
[14]周音频,黄 岚.血管内皮祖细胞动员在损伤血管修复中的作用及调控因素[J].中国动脉硬化杂志,2004,12(2):233-234.
[15]喻 杨,黄 岚.内皮祖细胞功能状态及其调节[J].中华心血管病杂志,2007,35(11):1067-1069.
[16]谢松涛,陈 璧,陶 克.人脐血来源内皮祖细胞的纯化鉴定及定向分化的研究[J].中华烧伤杂志,2006,22(5):355-358.
[17]Fox A,Smythe J,Fisher N,et al.Mobilization of endothelial progenitor cells into the circulation in burned patients[J].Br J Surg,2008,95(2):244-251.
[18]Groger A,Piatkowski A,Grieb G,et al.The mobilisation of mononuclear cells and endothelial progenitor cells after burn injury in a porcine model[J].Burns,2010,36(4):545-551.
[19]Foresta C,Schipilliti M,De Toni L,et al.Blood levels,apoptosis,and homing of the endothelial progenitor cells after skin burns and escharectomy[J].J Trauma,2011,70(2):459-465.
[20]李 静,陶维良,魏世东,等.重组人粒细胞集落刺激因子(rhG-CSF)的安全性与临床评价[J].中国医院用药评价与分析,2008,8(7):484-486.
[21]曹丽萍,邓诗琳.烧伤后血粒细胞集落刺激因子的变化及其与感染的关系[J].中华外科杂志,1995,33(7):403-405.
[22]张道坤,郑国平,杨建秋,等.粒细胞巨噬细胞集落刺激因子在烧伤治疗中的应用进展[J].中华烧伤杂志,2009,25(4):316-318.

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备注/Memo

备注/Memo:
国家重点基础研究发展计划(国家科技部973 计划，2009CB521902)；国家自然科学基金资助(81100866)
更新日期/Last Update: 2013-01-20