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[1]刘挺松,宫剑滨,程训民,等.波动性高糖对人脐静脉内皮细胞炎症因子表达的影响[J].医学研究与战创伤救治(原医学研究生学报),2013,15(03):209-211.[doi:10.3969/j.issn.1672-271X.2013.03.001]
　LIU Ting-song,GONG Jian-bin,CHENG Xun-min,et al.Impact of oscillating high glucose on pro-inflammatory cytokines expression in human umbilical vein endothelial cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(03):209-211.[doi:10.3969/j.issn.1672-271X.2013.03.001]
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波动性高糖对人脐静脉内皮细胞炎症因子表达的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第15卷
期数:
2013年03期
页码:
209-211
栏目:
出版日期:
2013-03-30

文章信息/Info

Title:
Impact of oscillating high glucose on pro-inflammatory cytokines expression in human umbilical vein endothelial cells
作者:
刘挺松; 宫剑滨; 程训民; 王立军; 彭永平; 刘 晶; 王 静; 江时森
210002 江苏南京,南京军区南京总医院心脏内科
Author(s):
LIU Ting-song; GONG Jian-bin; CHENG Xun-min; WANG Li-jun; PENG Yong-ping; LIU Jing; WANG Jing; JIANG Shi-sen
Department of Cardiology,Nanjing General Hospital of Nanjing Military Command,Nanjing,Jiangsu 210002,China
关键词:
波动性高糖; 内皮细胞; 炎症
Keywords:
oscillating high glucose;  endothelial cells;  inflammation
分类号:
R587.23
DOI:
10.3969/j.issn.1672-271X.2013.03.001
文献标志码:
A
摘要:
目的 研究体外波动性和持续性高糖对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)炎症因子表达的影响。方法 分别在正常葡萄糖(5.5 mmol/L,NG)、持续性高糖(25 mmol/L,PHG)、波动性高糖(5.5 mmol/L和 25 mmol/L葡萄糖每24 h交替,OHG)和波动性高渗环境(不加入葡萄糖,加入不同量甘露醇控制渗透压与波动性高糖组一致,OC)中连续培养HUVEC 7d。采用ELISA法和实时定量PCR法分别检测HUVEC在不同血糖环境中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和细胞间黏附因子-1(ICAM-1)中蛋白和mRNA的表达。结果 与NG组和OC组比较,发现持续性高糖可以显著升高HUVEC的IL-6、TNF-α 和ICAM-1蛋白表达(P<0.05),而波动性高糖可以进一步放大这种差异(P<0.05)。在mRNA水平,PHG组较NG组和OC组显著增加IL-6、TNF-α 和ICAM-1表达(P<0.05),而这一趋势在波动性高糖组最为明显(P<0.05)。结论 持续性高糖可以显著增强HUVEC炎症反应,而波动性高糖进一步放大这一效应。
Abstract:
Objective To investigate the inflammatory responses of human umbilical vein endothelial cells(HUVEC)to persistent and oscillating high glucose in vitro.Methods HUVEC was incubated for continuous 7 days in 5.5 mmol/L,25 mmol/L glucose,oscillating high(5.5 and 25 mmol/L glucose alternating every 24 hours)and mannitol respectively.Concentration of interleukin(IL)-6,tumor necrosis factor(TNF)-α and intercellular adhesion molecule-1(ICAM-1)proteins in the supernatants of cell culture were measured by use of ELISA kit.The mRNA of IL-6,TNF-α and ICAM-1 were evaluated by real-time quantitative PCR.Results Persistent high glucose caused a more intense inflammatory response than normal glucose and osmotic control in HUVEC with a marked increase of IL-6,TNF-α and ICAM-1 proteins in supernatants of cell culture(P<0.05),and the differences was amplified when cells cultured under oscillating high glucose.The concentration of the three pro-inflammatory cytokines mRNA were higher in cells exposed to oscillating high glucose than that exposed to persistent high glucose and normal glucose(P<0.05).Conclusion In cultured HUVEC,oscillating high glucose evoked more intense inflammatory response than persistent high glucose.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 中国博士后科学基金特别资助(2012T50897)
更新日期/Last Update: 2013-05-20