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[1]黄海嵘,张 雷,李德闽,等.大鼠体外循环后肺损伤的实验研究[J].医学研究与战创伤救治(原医学研究生学报),2014,16(02):113-116，162.[doi:10.3969/j.issn.1672-271X.2014.02.001]
　HUANG Hai-rong,ZHANG Lei,LI De-min,et al.Lung injury after cardiopulmonary bypass in rats[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(02):113-116，162.[doi:10.3969/j.issn.1672-271X.2014.02.001]
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大鼠体外循环后肺损伤的实验研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年02期
页码:
113-116，162
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Lung injury after cardiopulmonary bypass in rats
作者:
黄海嵘; 张 雷; 李德闽; 吴海卫; 易 俊; 申 翼; 董国华; 李忠东; 王常田
210002 江苏南京，南京军区南京总医院心胸外科
Author(s):
HUANG Hai-rong; ZHANG Lei; LI De-min; WU Hai-wei; YI Jun; SHEN Yi; DONG Guo-hua; LI Zhong-dong; WANG Chang-tian.
Department of Cardiothoracic Surgery,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,Jiangsu 210002,China
关键词:
体外循环; 炎症因子; 肺损伤
Keywords:
cardiopulmonary bypass; inflammatory cytokines; lung injury
分类号:
R654.1
DOI:
10.3969/j.issn.1672-271X.2014.02.001
文献标志码:
A
摘要:
目的 研究大鼠体外循环(cardiopulmonary bypass，CPB)后肺脏损伤及其相关机制。方法 24只雄性SD大鼠随机均分成两组:伪手术组(Sham组)和体外循环组(CPB组)，Sham组建立CPB模型管道，不进行体外循环；CPB组建立体外循环后流量逐步调至最大[≥100 ml/(kg·min)]维持转流60 min。于转流开始前(T0)和转流结束后(T1)、术后1 h(T2)、术后2 h(T3)、术后12 h(T4)行血清肿瘤坏死因子(tumor necrosis factor-α，TNF-α)、基质金属蛋白酶-9(matrix metalloproteinase-9，MMP-9)因子检测及动脉血气分析。术后12 h(T4)获取支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)和肺组织检测TNF-α、MMP-9浓度及肺组织和和核转录因子NF-κB(nuclear transcription factor-Kappa B,NF-κB)的表达水平，并行组织学检查。结果 CPB组CPB后大鼠血清、肺组织及BALF中炎症因子TNF-α、MMP-9均较Sham组明显上升，CPB组肺组织NF-κB蛋白表达水平显著高于Sham组(P＜0.05)；组织学检查发现CPB组表现为中性粒细胞浸润、肺间质水肿、出血、透明膜形成和坏死等病理变化。结论 CPB可导致肺组织NF-κB激活，上调TNF-α、MMP-9等炎症因子表达，引起肺损伤。
Abstract:
Objective To investigate the influence of cardiopulmonary bypass (CPB) on lung injury in rats and its related mechanism.Methods Twenty four male Sprague Dawley rats were randomly divided into two groups:sham operation group (Sham) and CPB group.Sham group establish CPB model,but without CPB bypass.After establish the CPB,the flow rate was gradually adjusted to the maximum[≥100 ml/(kg·min)] and maintained for 60 min.Blood samples were collected at the beginning and the end of CPB,1 h,2 h,12 h for assays of tumor necrosis factor-α (TNF-α),matrix metalloproteinase-9 (MMP-9) and arterial blood gas analysis.Bronchoalveolar lavage fluid (BALF) and lung tissue were obtained 12 h after the operation for detection of TNF-α,MMP-9 and NF-κB protein expression levels,histological examination was also performed.Results Compared with Sham group,inflammatory cytokines of TNF-α、MMP-9 of the CPB group in serum、lung tissue and BALF significantly increased and NF-κB protein expression levels in the lung samples were obviously higher after CPB (P<0.05).Histological examination revealed that the CPB group showed neutrophil infiltration,interstitial pulmonary edema,hemorrhage,hyaline membrane formation and necrosis.Conclusion Lung injury induced by CPB is related to NF-κB activation and thus increase of TNF-α、MMP-9.

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备注/Memo

备注/Memo:
国家自然科学基金(81101723)
更新日期/Last Update: 2014-03-20