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[1]郑敬民,尹 广,恽时锋,等.糖尿病肾病患者尿液糜蛋白酶检测及其病理意义分析[J].医学研究与战创伤救治(原医学研究生学报),2014,16(03):233-236.[doi:10.3969/j.issn.1672-271X.2014.03.003]
　ZHENG Jing-min,YIN Guang,YUN Shi-feng,et al.A study of urinary chymase expression in patients with diabetic nephropathy[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(03):233-236.[doi:10.3969/j.issn.1672-271X.2014.03.003]
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糖尿病肾病患者尿液糜蛋白酶检测及其病理意义分析()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年03期
页码:
233-236
栏目:
出版日期:
2014-05-20

文章信息/Info

Title:
A study of urinary chymase expression in patients with diabetic nephropathy
作者:
郑敬民; 尹 广; 恽时锋; 刘志红
210002 江苏南京，南京军区南京总医院国家肾脏疾病临床医学研究中心、全军肾脏病研究所
Author(s):
ZHENG Jing-min; YIN Guang; YUN Shi-feng; LIU Zhi-hong.
National Clinical Research Center of Kidney Diseases,Jinling Hospital,Nanjing University School of Medicine,Nanjing 210002,China
关键词:
糜蛋白酶; 肥大细胞; 糖尿病肾病;  病理意义
Keywords:
chymase;  mast cells;  diabetic nephropathy;  pathological significance
分类号:
R587.2;R692.9
DOI:
10.3969/j.issn.1672-271X.2014.03.003
文献标志码:
A
摘要:
目的 了解肥大细胞糜蛋白酶在糖尿病肾病患者尿液中的表达及其与糖尿病肾病临床指标的相关性，探讨糜蛋白酶和肥大细胞在糖尿病肾病肾组织损伤中的作用。方法 收 集糖尿病肾病103例(包括糖尿病肾病早期10例、中期31例和晚期62例)和20例正常对照者的尿液；利用ELISA法检测尿中糜蛋白酶水平；比较分析尿液糜蛋白酶在正常对照组、糖尿 病肾病早期组、中期组和晚期组中的变化情况及其与患者各临床指标的相关性。结果 与正常对照组相比，糖尿病肾病患者尿液中糜蛋白酶的水平显著升高，表现为随糖尿病肾病的 发展而逐渐增加的趋势。糖尿病肾病患者尿液糜蛋白酶水平与血肌酐[相关系数(r)=0.446,P<0.01]、血胱蛋白酶抑制剂C(r=0.398,P<0.01)、血尿素氮(r=0.365,P<0.01)、24 h尿蛋 白(r=0.512,P<0.01)、尿C3(r=0.321,P<0.01)、尿视黄醇结合蛋白(r=0.432,P<0.01)、尿溶菌酶(r=0.482,P<0.01)、尿N-乙酰-β-葡萄糖苷酶(r=0.582,P<0.01)水平具有显著相关 性。结论 上述结果支持关于肥大细胞参与糖尿病肾病肾组织损伤的观点，提示肾组织肥大细胞及其分泌的糜蛋白酶很可能在糖尿病肾病肾组织损伤中具有重要作用并可能是糖尿病 肾病防治的新靶标。
Abstract:
Objective To observe the expression of chymase in the urine of patients with diabetic nephropathy and its correlation with clinical indices.Methods Urine samples from 20 normal controls and 103 patients with diabetic nephropathy were collected.Among these patients,10 were identified to be at the early stage (microalbuminuria stage),31 were identified to be at the middle stage (proteinuria stage) and 62 were identified to be at the late stage (renal insufficiency stage).The level of chymase in each urine sample was measured by an ELISA kit.Its correlation with clinical indices was carried out.Results The urinary chymase level was found increased with the development of diabetic nephropathy.The urinary chymase level was found correlated significantly with serum creatinine,serum cystatin C,serum urea nitrogen,24 hour urine protein,urine C3,urine retinol-binding protein,urine lysozyme,urine N -acetyl-β-glucosaminidase level.Conclusion This study has provided another evidence for the involvement of mast cells in renal injury under the condition of diabetic nephropathy.Mast cells and chymase may have important roles in the development of diabetic nephropathy and might be a novel target for treatment of the disease.

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[18] 郑敬民,尹 广,姚根宏，等.肥大细胞在糖尿病肾病患者肾组织中的分布及相关性研究[J].医学研究生学报,2012,25(6):622-627.

相似文献/References:

[1]郑敬民,尹广,姚根宏,等.肾组织肥大细胞表型特点分析研究[J].医学研究与战创伤救治(原医学研究生学报),2013,15(02):97.
　Research Institute of Nephropathy,Jingling Hospital,Nanjing,et al.ZHENG Jing-min,YIN Guang,YAO Gen-hong,WANG Jian-ping,LIU Zhi-hong[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(03):97.

备注/Memo

备注/Memo:
江苏省自然科学基金资助项目(BK2012779)
更新日期/Last Update: 2014-05-20