|本期目录/Table of Contents|

[1]朱饮源,姚 军,李 曼.糖原合成酶激酶3β对卵巢癌紫杉醇耐药性的影响及机制研究[J].医学研究与战创伤救治(原医学研究生学报),2015,17(01):29-31，72.[doi:10.3969/j.issn.1672-271X.2015.01.009]
　ZHU Yin-yuan,YAO Jun,LI Man..The effects of GSK-3β on paclitaxel chemoresistance of ovarian cancer cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(01):29-31，72.[doi:10.3969/j.issn.1672-271X.2015.01.009]
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糖原合成酶激酶3β对卵巢癌紫杉醇耐药性的影响及机制研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年01期
页码:
29-31，72
栏目:
出版日期:
2015-01-31

文章信息/Info

Title:
The effects of GSK-3β on paclitaxel chemoresistance of ovarian cancer cells
作者:
朱饮源; 姚 军; 李 曼
541001 广西桂林，桂林医学院
Author(s):
ZHU Yin-yuan;  YAO Jun;  LI Man.
Guilin Medical University， Guilin， Guangxi 541001， China
关键词:
糖原合成酶激酶3β; 卵巢癌; 紫杉醇; 耐药性
Keywords:
GSK-3β;  ovarian cancer;  paclitaxel;  chemoresistance
分类号:
R737.31
DOI:
10.3969/j.issn.1672-271X.2015.01.009
文献标志码:
A
摘要:
目的 探讨糖原合成酶激酶3β(GSK-3β)对卵巢癌紫杉醇耐药性的影响及机制。方法 运用氯化锂(LiCl)增加卵巢癌细胞内失活性 GSK-3β的表达，MTT法测定紫杉醇的半数抑制浓度(IC50)，流式细胞仪检测p-GSK-3β(ser9)的表达量，免疫荧光观察其细胞内定位情况。结果 LiCl 20mmol/L处理1 h后，24、48、72 h SKOV3细胞紫杉醇的IC50从(10.11±0.87)、(1.07±0.17)、(0.03±0.01)μg/mL增加至(11.56±0.96)、(1.74±0.24)、(0.06±0.01)μg/mL，除24 h外差异均有统计学意义(P＜0.05)。LiCl处理后SKOV3细胞内p-GSK-3β(ser9)的平均荧光强度由(661.67±37.63)增加到(841.67±41.53)，差异有统计学意义(P＜0.05)。LiCl处理后SKOV3细胞核内p-GSK-3β(ser9)表达水平上调。结论 GSK-3β失活性磷酸化水平的增加，尤其是细胞核内表达水平的上调可导致卵巢癌细胞紫杉醇耐药性的增强。
Abstract:
Objective To study the effects of GSK-3β on paclitaxel chemoresistance of ovarian cancer cells. Methods SKOV3 cells was treated with LiCl 20 mmol/L for 1 hour to enhance the expression of p-GSK-3β (ser9) which is inactivated. MTT was used to test the IC50 of paclitaxel. The expression of p-GSK-3β (ser9) was tested by FACS. The location of p-GSK-3β (ser9) in SKOV3 cells was analyzed by immunofluorescence. Results After treated with LiCl， the IC50 of paclitaxel was increased from (10.11±0.87) μg/mL to (11.56±0.96) μg/mL for 24 h， (1.07±0.17) μg/mL to (1.74±0.24) μg/mL for 48 h， (0.03±0.01) μg/mL to (0.06±0.01) μg/mL for 72 h respectively. Except the increase of 24 h others had statistical difference(P＜0.05). FACS results show that the mean fluorescence intensity of p-GSK-3β increased from (661.67±37.63) to (841.67±41.53) after treated with LiCl(P＜0.05). The results of immunofluorescence show that the expression of p-GSK-3β in nuclear increased obviously than in the cytoplasm. Conclusion The augment of inactivated GSK-3β expression， especially in the nuclear， can improve the chemoresistance of ovarian cancer cells.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-01-20