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[1]余志阳,潘士勇,刘清珍,等.丙泊酚对帕金森小鼠黑质多巴胺能神经元凋亡的影响[J].医学研究与战创伤救治(原医学研究生学报),2015,17(04):346-348，371.[doi:10.3969/j.issn.1672-271X.2015.04.003]
　YU Zhi-yang,PAN Shi-Yong,LIU Qing-zhen,et al.The effect of propofol on apoptosis of dopaminergic neurons in the substantia nigra of mouse model of Parkinson’s disease[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(04):346-348，371.[doi:10.3969/j.issn.1672-271X.2015.04.003]
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丙泊酚对帕金森小鼠黑质多巴胺能神经元凋亡的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年04期
页码:
346-348，371
栏目:
出版日期:
2015-07-20

文章信息/Info

Title:
The effect of propofol on apoptosis of dopaminergic neurons in the substantia nigra of mouse model of Parkinson’s disease
作者:
余志阳1; 潘士勇2; 刘清珍1; 刘 健1; 陈春龙1; 李伟彦1; 朱四海1
210002 江苏南京，南京军区南京总医院，1.麻醉科，2.干部保健科
Author(s):
YU Zhi-yang1;  PAN Shi-Yong2;  LIU Qing-zhen1;  LIU Jian1;  CHEN Chun-long1;  LI Wei-yan1;  ZHU Si-hai1.
1.Department of Anesthesiology， 2.Department of Ranking Health Protection， Nanjing General Hospital of Nanjing Military Command， Nanjing， Jiangsu 210002， China
关键词:
帕金森病; 凋亡; 丙泊酚; 半胱氨酸蛋白酶-3; Bcl-2
Keywords:
Parkinsons disease;  apoptosis;  propofol;  cysteine protease-3;  Bcl-2
分类号:
R742.5
DOI:
10.3969/j.issn.1672-271X.2015.04.003
文献标志码:
A
摘要:
目的 观察丙泊酚对帕金森病(Parkinsons disease，PD)模型小鼠黑质多巴胺能神经元凋亡及半胱氨酸蛋白酶-3(caspase-3)、Bcl-2蛋白表达的影响。方法 雄性C57BL6小鼠48只，随机分为等渗盐水对照组、PD模型组、PD模型＋2 h脂肪乳组、PD模型＋24 h脂肪乳组、PD模型＋2 h丙泊酚组、PD模型＋24 h丙泊酚组。腹腔注射1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)30 mg/kg，连续5 d，建立小鼠PD模型。最后一次注射MPTP后分别于2 h、24 h腹腔注射脂肪乳(10 mL/kg)或丙泊酚(100 mg/kg)。最后一次腹腔注射药物后12 h后处死小鼠取中脑黑质区。采用免疫组化方法观察黑质区神经元凋亡情况，采用免疫蛋白印迹法检测Caspase-3、Bcl-2蛋白的表达水平。结果 与对照组比较，PD组模型及脂肪乳组，小鼠的黑质神经元凋亡细胞均明显增加，同时凋亡相关蛋白Caspase-3表达明显升高，而抗凋亡蛋白Bcl-2的水平明显下降。与脂肪乳组相比，丙泊酚组小鼠的黑质神经元凋亡细胞均明显减少，同时凋亡相关蛋白Caspase-3表达水平明显减低，而抗凋亡蛋白Bcl-2的水平明显上升，且分子表达改变在模型建立24 h后给予丙泊酚更显著。结论 丙泊酚能减少PD小鼠黑质多巴胺能神经元凋亡，同时上调抗凋亡蛋白Bcl-2表达，下调促凋亡蛋白Caspase-3蛋白的表达，丙泊酚通过调控凋亡相关蛋白表达水平产生脑保护作用。
Abstract:
Objective To observe the effect of propofol on neuronal apoptosis and expression of Caspase-3 and Bcl-2 protein in the substantia nigra of mouse model of Parkinsons disease. Methods A total of forty eight male C57BL6 mice were randomly assigned to six groups:group I control, the remaining five groups were firstly to establish mouse model of Parkinsons disease (PD) with intraperitoneal injection of 1-methyl -4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) 30 mg/kg for five consecutive days.Intralipos(10 mL/kg) or propofol (100 mg/kg) was respectively administered intraperitoneal injection in 2 h or 24 h after establishtion of PD.The mice were sacrificed to take the substantia nigra after the last 12 h intraperitoneal injection of drugs.Apoptosis was observed in substantia nigra with immunohistochemistry, and the expression of Bcl-2 and protein ysteine proteinase-3 were tested with Western blot. Results Compared with the mice in control group, the mice in PD group and intralipos group of apoptosis neurons were significantly increased in substantia nigra of mice, while the expression of protein Caspase-3 was increased significantly and the levels of the antiapoptotic protein Bcl-2 decreased obviously.The mice in popofol groups of apoptosis neurons were significantly decreased, compared with the mice in intralipos groups, however, expression of protein Caspase-3 apoptosis related was decreased significantly and the levels of the antiapoptotic protein Bcl-2 increased obviously, and the change of molecular expression was more significant in 24 h when the propofol was administered after establishment of PD model. Conclusion Propofol can reduce apoptosis in dopaminergic neurons PD of substantia nigra in PD mice, and upgrade expression of antiapoptotic protein Bcl-2, downgrade expression of apoptosis protein Caspase 3.Expression level of proteins apoptosis related was regulated to produce cerebral protection by propofol.

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备注/Memo

备注/Memo:
南京军区面上课题(12MA091)；南京军区南京总医院面上课题(2013026)
更新日期/Last Update: 2015-07-20